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  4. Recombinant Human A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1)

Recombinant Human A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1)

  • 货号：
    CSB-YP890696HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP890696HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP890696HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP890696HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP890696HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    A disintegrin and metalloproteinase with thrombospondin motifs 1; ADAM-TS 1; ADAM-TS1; ADAMTS 1; ADAMTS; ADAMTS-1; Adamts1; ATS1_HUMAN; C3 C5; KIAA1346,; METH 1; METH-1
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    253-967
  • 氨基酸序列
    FVSSHRYV ETMLVADQSM AEFHGSGLKH YLLTLFSVAA RLYKHPSIRN SVSLVVVKIL VIHDEQKGPE VTSNAALTLR NFCNWQKQHN PPSDRDAEHY DTAILFTRQD LCGSQTCDTL GMADVGTVCD PSRSCSVIED DGLQAAFTTA HELGHVFNMP HDDAKQCASL NGVNQDSHMM ASMLSNLDHS QPWSPCSAYM ITSFLDNGHG ECLMDKPQNP IQLPGDLPGT SYDANRQCQF TFGEDSKHCP DAASTCSTLW CTGTSGGVLV CQTKHFPWAD GTSCGEGKWC INGKCVNKTD RKHFDTPFHG SWGMWGPWGD CSRTCGGGVQ YTMRECDNPV PKNGGKYCEG KRVRYRSCNL EDCPDNNGKT FREEQCEAHN EFSKASFGSG PAVEWIPKYA GVSPKDRCKL ICQAKGIGYF FVLQPKVVDG TPCSPDSTSV CVQGQCVKAG CDRIIDSKKK FDKCGVCGGN GSTCKKISGS VTSAKPGYHD IITIPTGATN IEVKQRNQRG SRNNGSFLAI KAADGTYILN GDYTLSTLEQ DIMYKGVVLR YSGSSAALER IRSFSPLKEP LTIQVLTVGN ALRPKIKYTY FVKKKKESFN AIPTFSAWVI EEWGECSKSC ELGWQRRLVE CRDINGQPAS ECAKEVKPAS TRPCADHPCP QWQLGEWSSC SKTCGKGYKK RSLKCLSHDG GVLSHESCDP LKKPKHFIDF CTMAECS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cleaves aggrecan, a cartilage proteoglycan, at the '1938-Glu-|-Leu-1939' site (within the chondroitin sulfate attachment domain), and may be involved in its turnover. Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
  • 基因功能参考文献：
    1. we identified Adamts1 as the modulator of a potent pathway that converts changes in diet into a cellular signal in adipose tissue that controls APC activity in vivo PMID: 28700319
    2. ADAMTS 1 may be necessary during the decidualization and implantation stages of early normal pregnancy. PMID: 28949770
    3. Significant differences in gene expression profiles were observed between patients with post-kidney transplant bladder tumors and those with conventional bladder tumors. ADAMTS1 expression was significantly lower in first group than in the second. PMID: 29038419
    4. Our study demonstrated that altered levels of ADAMTS-1 and aggrecan may have a partial role in the etiopathogenesis of Polycystic ovary syndrome (PCOS), and ADAMTS-1 could be a predictive marker for implantation success in PCOS patients. PMID: 28417352
    5. Adamts1 acts as an extracellular matrix 'modifier', with miR-181d-induced downregulation, that regulates adipocyte lineage commitment and obesity. PMID: 27447109
    6. This study showed that ADAMTS1, 8, and 18 are highly expressed in GC and its nodal metastases, suggesting important roles of these proteases in carcinogenesis and lymphatic metastasis. The findings from the present study indicate that these proteases may be promising candidates for novel and alternative treatments in GC (gastric cancer) PMID: 28814085
    7. Taken together, our findings suggested that miR-362-3p inhibits the proliferation and migration of VSMCs by directly targeting ADAMTS1, which might provide novel insight into the molecular mechanisms underlying the action of miR-362-3p in atherosclerosis. PMID: 28890348
    8. ADAMTS1 has diverse roles in angiogenesis, tumor microenvironment and lymphangiogenesis. PMID: 28674292
    9. Patients with Marfan syndrome showed elevated NOS2 and decreased ADAMTS1 protein levels in the aorta. PMID: 28067899
    10. Data indicate that serum versican levels were significantly decreased in polycystic ovary syndrome (PCOS) patients, and that serum ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin motif-1) and versican levels were significantly and positively correlated with each other. PMID: 27908842
    11. Findings indicate that ADAMTS-1 has proteolytic functions in the nucleus through its interaction with aggrecan substrate in normal and tumoral breast cells. PMID: 27764205
    12. implicated in knee destruction in Kashin-Beck disease PMID: 27053287
    13. All ADAMTS tested in our study were expressed in both stable and unstable carotid plaques, especially in smooth muscle cells and macrophages. Analysis of the expression pattern on mRNA level showed significant higher expression of ADAMTS1 in unstable plaques compared with stable plaques. PMID: 25689086
    14. ADAMTS1 protein levels in semen were significantly lower in males with infertility. Sperm count and motility showed a negative correlation with levels of ADAMTS1 protein expression. PMID: 26888488
    15. increased expression of ADAMTS1 protein in macrophages and neutrophils that infiltrated aortic tissues may promote the progression of acute aortic dissection PMID: 26563155
    16. High ADAMTS1 expression was significantly associated with tumorigenicity of Kaposi's sarcoma-associated herpesvirus infected endothelial cells. PMID: 26675551
    17. Data suggest that the rs416905 and rs402007 single nucleotide polymorphisms of the a disintegrin and metallo-proteinase with thrombospondin type 1 motifs (ADAMTS-1) gene may be associated with ischemic stroke caused by large artery atherosclerosis (LAA). PMID: 26663063
    18. Results show that ADAMTS1 expression is negatively regulated in breast neoplasm cells due to mir-365 targeting its 3'UTR. PMID: 25998153
    19. The aberrant methylation of ADAMTS1 may be involved in the development and progression of gastric cancer. PMID: 25936341
    20. Ectopic expression of SP1 and USF transcription factors resulted in the decrease in ADAMTS1 transcriptional activity of all promoter constructs PMID: 26299656
    21. single-nucleotide missense mutation in the ADAMTS1 gene (c. 742I>T) was found to segregate in the family, given that the affected individuals must be heterozygous for the mutation PMID: 26124221
    22. Dysregulated expression of ADAMTS-1 in polycystic ovary syndrome may influence oocyte quality-via Granulosa cells-oocyte paracrine and endocrine mechanism. PMID: 24646063
    23. ADAMTS1 formed a complex with VEGFC, attenuated VEGFR3 phosphorylation and inhibited lymphangiogenesis in vitro. PMID: 24631293
    24. ADAMTS1 expression is decreased in breast tumor specimens. PMID: 23681936
    25. ADAMTS-1 expression was decreased in human breast tumors, and ADAMTS-1 knockdown stimulated migration, invasion and invadopodia formation in breast cancer cells in vitro. PMID: 23289900
    26. These findings suggest that ADAMTS1 expression is altered in primary gastric cancer and paired lymph node metastasis and has angioinhibitory effects. PMID: 23001403
    27. our results demonstrated that ADAMTS1 gene transfer inhibited angiogenesis in vitro and in vivo PMID: 22776012
    28. Pericyte-derived TIMP3 stabilized and ADAMTS1 destabilized the capillary tubular networks PMID: 22383695
    29. ADAMTS-1 was present in human coronary atherosclerotic plaques; ADAMDTS-1 was differentially expressed between acute myocardial infarction and stable angina; and ADAMTS-1 immunopositive cells were mainly macrophages. PMID: 21345877
    30. AHR, a novel acute hypoxia-response sequence, drives reporter gene expression under hypoxia in vitro and in vivo. PMID: 20795945
    31. The correlation between its expression in cumulus granulosa cells and oocyte fertilization capacity suggests a role for ADAMTS-1 in human cumulus function PMID: 20655981
    32. ADAMTS1 expression is elevated in endometrial adenocarcinoma PMID: 20840749
    33. ADAMTS1 is an important regulatory factor of angiogenesis and tumor growth in prostate tumors, where modified ADAMTS1 expression resulted in markedly changed blood vessel morphology, possibly related to altered thrombospondin 1 levels. PMID: 20546609
    34. Study documents the expression of ADAMTS1 in aggressive and highly plastic melanoma and Ewing sarcoma cells. Inhibiting ADAMTS1 compromised the endothelial mimetic attributes observed. PMID: 20484033
    35. results indicate that the antiangiogenic activity of TSP1 is differentially regulated by ADAMTS1 in the liver and lung, emphasizing the concept that regulation of angiogenesis is varied in different tissue environments. PMID: 20103648
    36. cleavage of semaphorin 3C induced by ADAMTS1 promotes the migration of breast cancer cells, indicating that the co-expression of these molecules in tumors may contribute to the metastatic program PMID: 19915008
    37. properties investigated through protein engineering PMID: 12054626
    38. cleaves aggrecan at multiple sites and is differentially inhibited by metalloproteinase inhibitors PMID: 12054629
    39. ADAMTS-1 is the first matrix-degrading enzyme downregulated by the catabolic factor IL-1beta in vitro. PMID: 14760803
    40. ADAMTS-1 expression was significantly reduced in the presence of HDL subfraction 3. PMID: 14996435
    41. results suggest that conserved residues other than the furin cleavage site in the prodomain of a disintegrin and metalloprotease with thrombospondin type I motif(ADAMTS-1) are involved in its biosynthesis PMID: 15184385
    42. negative effect of TGFbeta1 on ADAMTS-1, -5, -9, and -15 coupled with increases in their inhibitor, TIMP-3 may aid the accumulation of versican in the stromal compartment of the prostate in BPH and prostate cancer PMID: 15599946
    43. ADAMTS-1-immunoreactivity was manifold increased in brain with Down syndrome and neurodegeneration. PMID: 15661359
    44. specific distribution pattern of ADAMTS-1 in a variety of organs during embryogenesis suggests a role of the molecule in tissue remodelling, vasculogenesis and angiogenesis PMID: 15878613
    45. Expression of ADAMTS1 was downregulated by cAMP in normal, but not in SV40-transformed cells, suggesting that in normal cells epiregulin and amphiregulin activity is downregulated by a feedback mechanism that may be lost in SV40-transformed cells PMID: 15967414
    46. fibulin-1 is a new regulator of ADAMTS-1-mediated proteoglycan proteolysis and may play an important role in proteoglycan turnover in tissues where there is overlapping expression PMID: 16061471
    47. Overexpression of ADAMTS-1 promotes pulmonary metastasis of mammary carcinoma and Lewis lung carcinoma cells and that a proteinase-dead mutant of ADAMTS-1 inhibits their metastasis PMID: 16314835
    48. Has a previously unreported gelatinolytic activity. PMID: 16328961
    49. ADAMTS-1 expression is associated with decidualization of the endometrial stroma in vivo. IL-1beta increased whereas TGF-beta1 decreased ADAMTS-1 mRNA and protein levels in decidual stromal cell cultures in a concentration- and time-dependent manner. PMID: 16675485
    50. Hypermethylation was subsequently identified for three of four analyzed genes, ADAMTS1 (85%), CRABP1 (90%), and NR3C1 (35%). PMID: 17167179

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 数据库链接：

    HGNC: 217

    OMIM: 605174

    KEGG: hsa:9510

    STRING: 9606.ENSP00000284984

    UniGene: Hs.643357