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  4. Recombinant Escherichia coli Uracil-DNA glycosylase (ung)

Recombinant Escherichia coli Uracil-DNA glycosylase (ung)

  • 货号：
    CSB-YP025641ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025641ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025641ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025641ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025641ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ung; b2580; JW2564; Uracil-DNA glycosylase; UDG; EC 3.2.2.27
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-229
  • 氨基酸序列
    ANELTWHDV LAEEKQQPYF LNTLQTVASE RQSGVTIYPP QKDVFNAFRF TELGDVKVVI LGQDPYHGPG QAHGLAFSVR PGIAIPPSLL NMYKELENTI PGFTRPNHGY LESWARQGVL LLNTVLTVRA GQAHSHASLG WETFTDKVIS LINQHREGVV FLLWGSHAQK KGAIIDKQRH HVLKAPHPSP LSAHRGFFGC NHFVLANQWL EQRGETPIDW MPVLPAESE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.
  • 基因功能参考文献：
    1. A mutant uracil DNA glycosylase distinguishes between cytosine and 5-methylcytosine. PMID: 24740413
    2. Contrasting effects of mutating active site residues, aspartic acid 64 and histidine 187 of Escherichia coli uracil-DNA glycosylase on uracil excision and interaction with an inhibitor protein PMID: 22905376
    3. oligonucleotide substrates containing two uracil residues in defined positions were used to characterize one-dimensional search of DNA by Escherichia coli Ung. PMID: 21971549
    4. The significance of the Ung residency on the AP-site in the uracil excision repair pathway in E. coli. PMID: 20056657
    5. negatively regulated by the two-component system of CpxR/CpxA signal transduction. PMID: 15576781
    6. A synthetic DNA duplex of uracil DNA glycosylase contains an excellent isostere of uracil but lacks hydrogen bonds and acceptor groups; it attains a metastable unstacked state that mimics a previously detected intermediate on the uracil-flipping pathway. PMID: 15581354
    7. The structure of native and modified uracil DNA glycosylase from E. coli in solution was studied by synchrotron small-angle X-ray scattering. PMID: 16521548
    8. influence of the ung, dug, and dut genes in preventing uracil residues from accumulating in E. coli DNA was quantitatively determined PMID: 16908222
    9. for the human and Escherichia coli UNG enzymes, discrimination of thymine and uracil is initiated by thermally induced opening of T*A and U*A base pairs and not by active participation of the enzyme PMID: 17704764

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Uracil-DNA glycosylase (UDG) superfamily, UNG family
  • 数据库链接：

    KEGG: ecj:JW2564

    STRING: 316385.ECDH10B_2748