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  4. Recombinant Drosophila melanogaster Vacuolar protein-sorting-associated protein 25 (Vps25)

Recombinant Drosophila melanogaster Vacuolar protein-sorting-associated protein 25 (Vps25)

  • 货号：
    CSB-YP741957DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP741957DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP741957DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP741957DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP741957DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Vps25; l(2)44Db; CG14750Vacuolar protein-sorting-associated protein 25; ESCRT-II complex subunit VPS25; Vacuolar protein sorting 25
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-174
  • 氨基酸序列
    MAEFQWPWEY TFPPFFTLQP HEETRQQQLK VWTDLFLKYL RHTNRFTLSI GDQNSPLFHN EALKRRLSPE LVLAILGELE RSGHANPLDK RRQEWQVYWF TLEEYGNMVY DWVQETGQTN TICTLYEIAS GENTSHLDFY GVDEAVLLSA LRLLEEKGRC ELIEMDGSHG VKFF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Seems to function as a tumor suppressor by regulating Notch trafficking, hence preventing non-autonomous overproliferation. May be involved in the regulation of autophagy. ESCRT-II interacts with bicoid mRNA, which is required for the anterior localization of bicoid mRNA in the developing egg.
  • 基因功能参考文献：
    1. ESCRT-II mutant tissues have deregulated signal transduction pathways, leading to tumorigenesis. PMID: 23418496
    2. The non-cell autonomous induction of Yorkie is due to Notch signaling from vps25 mutant cells. PMID: 22679484
    3. The vps25 protein, a component of the ESCRT machinery that regulates endocytic sorting of signaling receptors, as an unconventional type of Drosophila tumor suppressor. PMID: 16256743
    4. The vps25 protein encodes a component of the ESCRT-II complex, which sorts membrane proteins into multivesicular bodies during endocytic trafficking to the lysosome. PMID: 16256745
  • 亚细胞定位：
    Cytoplasm. Endosome membrane.
  • 蛋白家族：
    VPS25 family
  • 数据库链接：

    KEGG: dme:Dmel_CG14750

    STRING: 7227.FBpp0087774

    UniGene: Dm.4897