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  4. Recombinant Danio rerio T-cell acute lymphocytic leukemia protein 1 homolog (tal1)

Recombinant Danio rerio T-cell acute lymphocytic leukemia protein 1 homolog (tal1)

  • 货号：
    CSB-YP527150DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP527150DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP527150DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP527150DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP527150DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tal1
  • Uniprot No.：
  • 别名：
    tal1; scl; tal-1; si:ch211-135j1.3; T-cell acute lymphocytic leukemia protein 1 homolog; TAL-1; Stem cell protein; zSCL
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-324
  • 氨基酸序列
    MMEKLKSEQF PLSPSAEGCA SPPRGDGDAR GKQEGTTAET GEHRLPEELN GVAKETAHHA TELKKEVAVI ELSRRGGSAD IKGRELKAEL SHKVQTTELC RPPIPLPLPP RDPLSDTRMV QLSPPAFPLP ARAMLYSNMT TPLATINSGF AGDAEQYGMY PSNRVKRRPA PYEVEINDGS QPKIVRRIFT NSRERWRQQN VNGAFAELRK LIPTHPPDKK LSKNEILRLA MKYINFLAKL LNDQDDMVGG EAPARANRDS RDATLVRDDL LQEMLSPNSS CGSLLDGDAS PESFTEDQDS SVESRPSARG LHHSSLPLDG NAQR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that plays a pivotal role in hemopoietic and endothelial development, acting synergistically with lmo2 and downstream of clo. Specifies mesodermal precursors to a hemangioblast cell fate. Hemangioblasts are bipotential precursors of blood and endothelium, and in the absence of hemopoietic induction cues such as gata1, tal1/scl-lmo2-induced hemangioblasts differentiate into endothelial cells. Isoform alpha and isoform beta are redundant for the initiation of primitive hemopoiesis but have distinct roles in the regulation of primitive erythroid differentiation and definitive hemopoietic stem cell specification, most likely due to differences in expression levels. Specification of definitive hemopoietic stem cells requires isoform beta. DNA binding is required for erythroid maturation, but not for its other hemopoietic functions. Endothelial roles include development of the dorsal aorta, the site of definitive hemopoiesis in the embryo. Required for angiogenesis but not angioblast specification. Has an additional role in endocardium formation during heart development. May play a role in central nervous system development.
  • 基因功能参考文献：
    1. The latter data suggest that Stil play a role in retina cell proliferation through the Shh signal transduction pathway. PMID: 24469449
    2. Tal1 has distinct roles in regulating the formation of endocardial intercellular junctions. PMID: 24075907
    3. Data suggest that a defined hemogenic endothelial population preset by scl-beta supports the deterministic emergence of HSCs, and unravel the cellular mechanisms by which scl isoforms regulate HSC development. PMID: 24046317
    4. Our studies establish a new connection between RA and scl during development that may participate in stem cell self-renewal and hematopoietic differentiation PMID: 20410509
    5. Ets1-related protein plays a critical role in the initiation of definitive hematopoiesis by controlling the expression of 2 stem cell leukemia (scl) isoforms (scl-alpha and scl-beta) in angioblasts. PMID: 20185582
    6. in the absence of inducers of erythroid or myeloid haematopoiesis, Scl/Tal1-Lmo2-induced haemangioblasts differentiate into endothelial cells PMID: 14602685
    7. Zebrafish scl has a role in hematopoietic and endothelial development, downstream of hemangioblast development. PMID: 15617691
    8. scl is especially critical for the development of arteries where adult hematopoietic stem cells emerge, implicating scl in the formation of hemogenic endothelium PMID: 15644413
    9. Scl participates in multiple processes requiring different levels and functions PMID: 16210319
    10. While scl-alpha and -beta are redundant for the initiation of primitive hematopoiesis, these two isoforms exert distinct functions in the regulation of primitive erythroid differentiation and definitive hematopoietic stem cell specification. PMID: 17472439
    11. In germline-transgenic zebrafish generated by inserting GFP into the first coding exon of scl, GFP expression completely recapitulates the endogenous expression of scl in blood, endothelium and CNS. PMID: 17559829
    12. Scl/Tal1 is an indispensable component of the molecular hierarchy that controls endocardium morphogenesis. PMID: 17722983
    13. These results demonstrate that scl is an important mediator of the ability of AML1-ETO to reprogram hematopoietic cell fate decisions, suggesting that scl may be an important contributor to AML1-ETO-associated leukemia. PMID: 18156164

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in hemopoietic and endothelial lineages. Isoform beta emerges first, expressing in the entire anterior and posterior lateral mesoderm (ALM and PLM respectively), and in the ventral wall of the dorsal aorta, where definitive hemopoiesis begins. I
  • 数据库链接：

    KEGG: dre:30766

    STRING: 7955.ENSDARP00000077498

    UniGene: Dr.75812