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  4. Recombinant Chicken Histone H3-like centromeric protein A (CENPA)

Recombinant Chicken Histone H3-like centromeric protein A (CENPA)

  • 中文名称：
    Recombinant Chicken Histone H3-like centromeric protein A(CENPA),Yeast
  • 货号：
    CSB-YP754473CH
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Chicken Histone H3-like centromeric protein A(CENPA),Yeast
  • 货号：
    CSB-EP754473CH
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Chicken Histone H3-like centromeric protein A(CENPA),Yeast
  • 货号：
    CSB-EP754473CH-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Chicken Histone H3-like centromeric protein A(CENPA),Yeast
  • 货号：
    CSB-BP754473CH
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Chicken Histone H3-like centromeric protein A(CENPA),Yeast
  • 货号：
    CSB-MP754473CH
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CENPA
  • Uniprot No.：
  • 别名：
    CENPAHistone H3-like centromeric protein A; Centromere protein A; CENP-A; GgCENP-A
  • 种属：
    Gallus gallus (Chicken)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-131
  • 氨基酸序列
    MPRPKPRSPR RRGRPPPAAP PPPPARPRAR RYRPGQRALR EIRRYQSSTA LLLRRQPFAR VVREICLLFT RGVDYRWQAM ALLALQEAAE AFLVHLLEDA YLCSLHARRV TLYPKDLQLA RRLRGLQGEG F
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis.
  • 基因功能参考文献：
    1. M18BP1/KNL2 is essential for centromere formation through direct binding to CENP-A nucleosome in non-mammalian vertebrates. PMID: 28743004
    2. CCAN has two distinct roles: recruiting CENP-A to establish the kinetochore and serving as a structural core to directly recruit kinetochore proteins. PMID: 23277427
    3. Data show that CENP-A induces some positioning of its own and some unwrapping at the entry-exit relative to canonical nucleosomes on both 5 S DNA and the alpha-satellite sequence on which it is normally loaded. PMID: 17524417
  • 亚细胞定位：
    Nucleus. Chromosome, centromere, kinetochore. Chromosome, centromere.
  • 蛋白家族：
    Histone H3 family
  • 数据库链接：

    KEGG: gga:100113359