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  4. Recombinant Bacillus subtilis Alanine-anticapsin ligase BacD (bacD)

Recombinant Bacillus subtilis Alanine-anticapsin ligase BacD (bacD)

  • 中文名称：
    Recombinant Bacillus subtilis Alanine-anticapsin ligase BacD(bacD),Yeast
  • 货号：
    CSB-YP336605BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Bacillus subtilis Alanine-anticapsin ligase BacD(bacD),Yeast
  • 货号：
    CSB-EP336605BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Bacillus subtilis Alanine-anticapsin ligase BacD(bacD),Yeast
  • 货号：
    CSB-EP336605BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Bacillus subtilis Alanine-anticapsin ligase BacD(bacD),Yeast
  • 货号：
    CSB-BP336605BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Bacillus subtilis Alanine-anticapsin ligase BacD(bacD),Yeast
  • 货号：
    CSB-MP336605BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    bacD
  • Uniprot No.：
  • 别名：
    bacD; ywfE; BSU37710; ipa-83dAlanine--anticapsin ligase; EC 6.3.2.49; ATP-dependent dipeptide ligase; Bacilysin synthetase; L-Ala-L-amino acid dipeptide ligase; L-alanine--L-anticapsin ligase; L-amino acid ligase; Lal
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-472
  • 氨基酸序列
    MERKTVLVIA DLGGCPPHMF YKSAAEKYNL VSFIPRPFAI TASHAALIEK YSVAVIKDKD YFKSLADFEH PDSIYWAHED HNKPEEEVVE QIVKVAEMFG ADAITTNNEL FIAPMAKACE RLGLRGAGVQ AAENARDKNK MRDAFNKAGV KSIKNKRVTT LEDFRAALEE IGTPLILKPT YLASSIGVTL ITDTETAEDE FNRVNDYLKS INVPKAVTFE APFIAEEFLQ GEYGDWYQTE GYSDYISIEG IMADGEYFPI AIHDKTPQIG FTETSHITPS ILDEEAKKKI VEAAKKANEG LGLQNCATHT EIKLMKNREP GLIESAARFA GWNMIPNIKK VFGLDMAQLL LDVLCFGKDA DLPDGLLDQE PYYVADCHLY PQHFKQNGQI PETAEDLVIE AIDIPDGLLK GDTEIVSFSA AAPGTSVDLT LFEAFNSIAA FELKGSNSQD VAESIRQIQQ HAKLTAKYVL PV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the bacABCDEFG operon responsible for the biosynthesis of bacilysin, an irreversible inactivator of the glutaminase domain of glucosamine synthetase. Catalyzes the formation of alpha-dipeptides from various L-amino acids in the presence of ATP. In vivo catalyzes the ligation of L-alanine and L-anticapsin (epoxycyclohexanonyl-Ala) to produce the final bacilysin antibiotic (L-Ala-L-4S-cyclohexenonyl-Ala dipeptide). The substrate specificity is restricted to small amino acids such as L-Ala, for the N-terminal end of the dipeptide, whereas a wide range of hydrophobic amino acids such as L-Phe, L-Tyr and L-Met are recognized for the C-terminal end.
  • 基因功能参考文献：
    1. Crystal structure of BacD with ADP suggested that Arg328 is a crucial residue for L-Ala recognition and catalysis. PMID: 24702628
  • 数据库链接：

    KEGG: bsu:BSU37710

    STRING: 224308.Bsubs1_010100020371