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  4. Recombinant Arabidopsis thaliana Protein ABSCISIC ACID-INSENSITIVE 5 (ABI5)

Recombinant Arabidopsis thaliana Protein ABSCISIC ACID-INSENSITIVE 5 (ABI5)

  • 货号：
    CSB-YP879765DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP879765DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP879765DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP879765DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ABI5
  • Uniprot No.：
  • 别名：
    ABI5; BZIP39; DPBF1; GIA1; NEM1; At2g36270; F2H17.12Protein ABSCISIC ACID-INSENSITIVE 5; Dc3 promoter-binding factor 1; AtDPBF1; Protein GROWTH-INSENSITIVITY TO ABA 1; bZIP transcription factor 39; AtbZIP39
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-442
  • 氨基酸序列
    MVTRETKLTS EREVESSMAQ ARHNGGGGGE NHPFTSLGRQ SSIYSLTLDE FQHALCENGK NFGSMNMDEF LVSIWNAEEN NNNQQQAAAA AGSHSVPANH NGFNNNNNNG GEGGVGVFSG GSRGNEDANN KRGIANESSL PRQGSLTLPA PLCRKTVDEV WSEIHRGGGS GNGGDSNGRS SSSNGQNNAQ NGGETAARQP TFGEMTLEDF LVKAGVVREH PTNPKPNPNP NQNQNPSSVI PAAAQQQLYG VFQGTGDPSF PGQAMGVGDP SGYAKRTGGG GYQQAPPVQA GVCYGGGVGF GAGGQQMGMV GPLSPVSSDG LGHGQVDNIG GQYGVDMGGL RGRKRVVDGP VEKVVERRQR RMIKNRESAA RSRARKQAYT VELEAELNQL KEENAQLKHA LAELERKRKQ QYFESLKSRA QPKLPKSNGR LRTLMRNPSC PL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.
  • 基因功能参考文献：
    1. SOM is reported to negatively regulate seeds germination by altering GA/ABA metabolism, here we found that AFP2 and ABI5 altered SOM transcription. Data demonstrate that AFP2 is a novel regulator to control high temperature-induced secondary seed dormancy through ABI5 and SOM. PMID: 29723526
    2. These data demonstrate that PHO1 is involved in ABA-mediated seed germination and early seedling development and transcriptionally regulated by ABI5. PMID: 29089393
    3. Arabidopsis MADS-Box Transcription Factor AGL21 Acts as Environmental Surveillance of Seed Germination by Regulating ABI5 Expression PMID: 28438576
    4. ABI5 is a direct target gene of BZR1, and modulating the expression of ABI5 by BZR1 plays important roles in regulating the crosstalk between the brassinosteroid and abscisic acid signalling pathways. PMID: 27149247
    5. NF-YC9 mediates abscisic acid (ABA) signaling via targeting to and aiding the ABA-responsive transcription factors such as ABI5. PMID: 28924726
    6. All of the study findings suggest that ABI5 regulates seed germination at least partly by affecting ROS homeostasis. PMID: 28391398
    7. These results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination. PMID: 26564029
    8. DOG1 affects the expression of hundreds of genes including LATE EMBRYOGENESIS ABUNDANT and HEAT SHOCK PROTEIN genes which are affected by DOG1 partly via control of ABI5 expression. PMID: 26729600
    9. ABI5 bind to the ABR promoter, indicating that ABI5 directly regulates the expression of ABR. The disruption of ABI5 function in abr abi5-1 plants abolished the senescence-accelerating phenotype of the abr mutant, demonstrating that ABI5 is epistatic to ABR. PMID: 27095403
    10. RGL2 promotes the entrance in secondary dormancy through ABI5 action. PMID: 26177669
    11. link between nitric oxide and abscisic acid hormone signalling through distinct posttranslational modifications of ABI5 during early seedling development PMID: 26493030
    12. RAV1 plays an important role in abscisic acid signaling by modulating the expression of ABI3, ABI4, and AbI5 during seed germination and early seedling development. PMID: 25231920
    13. BIN2 phosphorylates and stabilizes ABI5 to mediate Abscisic acid (ABA) response during seed germination, while brassinosteroids (BRs) repress the BIN2-ABI5 cascade to antagonize ABA-mediated inhibition. PMID: 25415975
    14. ubiquitination promotes AB15 degradation; sumoylation prohibits degradation PMID: 24398698
    15. results suggest that ABA negatively regulates SHB1 expression, at least in part, through the action of its downstream signaling component ABI5 PMID: 24619610
    16. Transgenic cotton (Gossypium hirsutum) expressing AtRAV1/2 and/or AtABI5 showed resistance to imposed drought stress. PMID: 24483851
    17. ABI5 functions in the glucose-mediated inhibition of the root meristem zone by repressing PIN1 accumulation, thus leading to reduced auxin levels in roots. PMID: 24237322
    18. our study indicates that BBX21 coordinates with HY5 and ABI5 on the ABI5 promoter and that these transcriptional regulators work in concert to integrate light and ABA signaling in Arabidopsis thaliana. PMID: 24586210
    19. These results define a genetic pathway through which cytokinin specifically induces the degradation of ABI5 protein. PMID: 24443524
    20. Overexpression of TAP46 and inhibition of activities of PP2A or PP2A-like protein phosphatases can increase transcript levels of several ABI5-regulated genes. PMID: 24357600
    21. The function of AtSAG in abscisic acid (ABA)signalling depended on ABI3 and ABI5. AtSAG is an important negative regulator of ABA signalling during seed germination and seedling development. PMID: 24163287
    22. These data suggest that Ala214 in ABI5 contributes to the function of ABI5 via its interaction with ABI3. PMID: 23299338
    23. ABI5 phosphorylation is regulated by PP6 phosphatase. PMID: 23404889
    24. Data indicate that KEEP ON GOING (KEG) E3 interacts with abscisic acid insensitive 5 (ABI5) in the cytoplasm. PMID: 23720747
    25. The inhibitory effect of abscisic acid on floral transition is mediated by ABI5. PMID: 23307919
    26. MED25 physically associates with the basic Leu zipper transcription factor ABA-INSENSITIVE5 (ABI5) in promoter regions of ABI5 target genes and shows a negative effect on ABI5-regulated gene transcription. PMID: 22822206
    27. one-hybrid assays demonstrated synergistic action of ABI4 with ABI5 or related bZIP factors in regulating target promoters, and mutant analyses showed that ABI4 and these bZIPs share some functions PMID: 21243515
    28. Abscisic acid promotes ABI5 accumulation by inducing the ubiquitination and proteasomal degradation of KEG. PMID: 20682837
    29. The redundancy of function among genes in the families of ABF3 and ABI5 in A. thaliana is reported. PMID: 16247556
    30. Results show that over-expression of ABI5 rendered phas expression ABA-independent in the presence of PvALF. PMID: 18038114
    31. PRT6 control of germination and establishment, as exemplified by ABA and sugar sensitivity, as well as storage oil mobilization, occurs at least in part via transcription factors ABI3 and ABI5. PMID: 19255443
    32. SIZ1-dependent sumoylation of ABI5 attenuates abscisic acid signaling PMID: 19276109

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BZIP family, ABI5 subfamily
  • 组织特异性：
    Predominantly expressed in seeds.
  • 数据库链接：

    KEGG: ath:AT2G36270

    STRING: 3702.AT2G36270.1

    UniGene: At.14114