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  4. Recombinant Apis mellifera Phospholipase A2

Recombinant Apis mellifera Phospholipase A2

In Stock
  • 货号：
    CSB-EP018091DNK
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Phospholipase A2; bvPLA2; EC 3.1.1.4; Allergen Api m I; Phosphatidylcholine 2-acylhydrolase; allergen Api m 1
  • 种属：
    Apis mellifera (Honeybee)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    19.3 kDa
  • 表达区域：
    34-167aa
  • 氨基酸序列
    IIYPGTLWCGHGNKSSGPNELGRFKHTDACCRTHDMCPDVMSAGESKHGLTNTASHTRLSCDCDDKFYDCLKNSADTISSYFVGKMYFNLIDTKCYKLEHPVTGCGERTEGRCLHYTVDKSKPKVYQWFDLRKY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer，50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • 基因功能参考文献：
    1. PLA(2)s were located on the membrane surface rather than penetrating the membrane bilayer and that the deeply inserted mode is not a favorable condition for the hydrolysis of phospholipids in zwitterionic phospholipid membranes. PMID: 21557547
    2. contributions of the individual disulfide bonds to conformational stability PMID: 20884319
    3. successive introduction of sequence modifications outside the human and murine T cell epitope regions led to a progressive loss of specific IgE and IgG reactivity PMID: 15459335
    4. Studies with bee venom (bv) mutant PLA2 show that electrostatic interactions between lysine and arginine residues and anionic phosphatidylserine contribute minimally to overall interfacial binding energetics of bvPLA2. PMID: 15491136
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Phospholipase A2 family, Group III subfamily
  • 组织特异性：
    Expressed by the venom gland.
  • 数据库链接：

    KEGG: ame:406141

    STRING: 7460.GB13351-PA

    UniGene: Ame.2