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TOMM22 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA277640A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, L02 whole cell lysate, HepF2 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
      All lanes: TOMM22 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 16 kDa
      Observed band size: 18 kDa
    • IHC image of CSB-RA277640A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    TOMM22 encodes TOM22, an essential component of the protein translocation complex (Tom complex) of the mitochondrial outer membrane. TOM22 serves as the central receptor for both presequence precursors and carrier precursors. The N-terminal domain of TOM22 functions as a preprotein receptor in cooperation with TOM20. In addition to functioning as an import receptor, TOM22 forms a conducting channel with TOM40, a major component of the general insertion pore. TOM22 deficiency might induce cardiomyocyte dysfunction by interfering with cardiac mitochondrial Ca2+ import.

    The vectors expressing anti-TOMM22 antibody were constructed as follows: immunizing an animal with A synthesized peptide derived from human TOMM22, isolating the positive splenocyte and extracting RNA, obtaining DNA by reverse transcription, sequencing and screening TOMM22 antibody gene, and amplifying heavy and light chain sequence by PCR and cloning them into plasma vectors. After that, the vector clones were transfected into the mammalian cells for production. The product is the recombinant TOMM22 antibody. Recombinant TOMM22 antibody in the culture medium was purified using Affinity-chromatography. It can react with TOMM22 protein from Human and is used in the ELISA, WB, IHC.

  • Uniprot No.：
    Q9NS69
  • 基因名：
    TOMM22
  • 别名：
    Mitochondrial import receptor subunit TOM22 homolog (hTom22) (1C9-2) (Translocase of outer membrane 22 kDa subunit homolog), TOMM22, TOM22
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human TOMM22
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    7C11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Central receptor component of the translocase of the outer membrane of mitochondria (TOM complex) responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with the peripheral receptor TOM20 functions as the transit peptide receptor and facilitates the movement of preproteins into the translocation pore. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
  • 基因功能参考文献：
    1. A directed proteomic approach discovered the novel interaction of BKCa with Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT). PMID: 27592226
    2. Tom22 is essential for metabolic conversion, as its knockdown by small interfering RNA (siRNA) completely ablated progesterone conversion in both steroidogenic mouse Leydig MA-10 and human adrenal NCI cells. PMID: 26787839
    3. Blockade of mitochondrial protein import triggers the recruitment of PARK2, by PINK1, to the TOMM machinery. PMID: 24149440
    4. Studies indicate that PUMA and TOMM22 as potential targets for miRNA-BART viral transcripts. PMID: 22178394
    5. The interaction with the cytosolic domain of Tom22 helps Bax to acquire a conformation able to interact with the outer mitochondrial membrane. PMID: 22198199
    6. the cytosolic domains of Tom20 and Tom22 function to maintain their substrate preproteins unfolded and prevent them from aggregating on the mitochondrial surface PMID: 14699115
    7. We have identified TOM22, a component of the translocase of the outer mitochondrial membrane (TOM), as a mitochondrial receptor of Bax. PMID: 17096026

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  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass membrane protein.
  • 蛋白家族：
    Tom22 family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 18002

    OMIM: 607046

    KEGG: hsa:56993

    STRING: 9606.ENSP00000216034

    UniGene: Hs.595072