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  4. TACC3 Recombinant Monoclonal Antibody

TACC3 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA553945A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, THP-1 whole cell lysate
      All lanes: TACC3 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 91 kDa
      Observed band size: 140 kDa
    • IHC image of CSB-RA553945A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    TACC3 regulates spindle organization during mitosis and also regulates centrosome-mediated microtubule nucleation by affecting γ-Tubulin ring complexes. In addition, it interacts with different proteins such as ch-TOG, clathrin, and Aurora-A, to function in mitotic spindle assembly and stability. By forming the TACC3/ch-TOG complex, TACC3 acts as a plus end-tracking protein to promote microtubule elongation. Aberrant expression of TACC3 has been observed in various human cancers and has been shown to promote tumor growth in vivo. In human cells, TACC3 has been shown to be associated with the organization of the mitotic spindle poles. Subsequent studies revealed that TACC3 depletion induced chromosomal misalignment reduced spindle stability, and triggered apoptosis in cells.

    The recombinant TACC3 antibody was produced by cloning antibody genes into an expression vectors, which were subsequently introduced into mammalian cells to provide animal-free antibody production. This TACC3 antibody has been validated in ELISA, WB, IHC. It has the features of improved affinity, stability, and consistency between different batches.

  • Uniprot No.：
    Q9Y6A5
  • 基因名：
    TACC3
  • 别名：
    Transforming acidic coiled-coil-containing protein 3 (ERIC-1), TACC3, ERIC1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human TACC3
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    10G7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. May be involved in the control of cell growth and differentiation. May contribute to cancer.
  • 基因功能参考文献：
    1. TACC3 was increased in human RCC cell lines, and knockdown of TACC3 inhibited the ability of cell proliferation, migration, invasion, and tumorigenesis in vivo. PMID: 28109075
    2. this study suggests that TACC3 might be an important molecular marker for diagnosis and prognosis of breast cancer PMID: 27258563
    3. Results show that TACC3 region of FGFR3-TACC3 gene fusion can bind and recruit endogenous TACC3 from the spindle. This reduction in TACC3 levels on the mitotic spindle is the primary cause of mitotic defects in urinary bladder. PMID: 28855393
    4. FGFR3-TACC3 is a recurrent resistance mechanism, which can bypass EGFR blockade by all generations of EGFR TKIs in NSCLC. PMID: 28838400
    5. TACC3 rs798766 is associated with increased risk of bladder cancer, and no ethnic difference was found. PMID: 28655970
    6. High levels of TACC3 in human mammary epithelial cells can cause genomic instability possibly in part through destabilizing BRCA1. PMID: 29355525
    7. TACC3 was highly expressed in CCA tissues and predicted a poor prognosis in CCA patients. TACC3 knockdown induced G2/M cycle arrest and suppressed the invasion, metastasis, and proliferation of CCA cells, both in vitro and in vivo. PMID: 27705912
    8. Data indicate that transforming acidic coiled-coil protein-3 (TACC3) promotes CRC progression and could be an independent prognostic factor and a potential therapeutic target for colorectal cancer (CRC). PMID: 27248823
    9. results suggest that TACC3 is an independent prognostic factor and may be a novel therapeutic target for the treatment of ST PMID: 29135996
    10. The combination treatment of SNIPER(TACC3) and bortezomib exhibited a synergistic anticancer activity in several cancer cell lines. PMID: 28192613
    11. Study provides evidence of the significant oncogenic potential of the FGFR3-TACC3 fusion protein. The presence of the TACC coiled-coil domain leads to increased and altered levels of FGFR3 activation, fusion protein phosphorylation, downstream signaling, cellular transformation, proliferation, and viability. PMID: 26869289
    12. Taken together, these findings uncover a supportive role for TACC3 in PCa metastasis, which is mediated by the activation of the Wnt/beta-catenin signaling pathway, suggesting that TACC3 may serve as a prognostic marker in patients with metastatic PCa. PMID: 28336437
    13. our observations identify TACC3 as an oncogene of tumor malignancy, as well as a prognostic and motoring biomarker for glioma patients. PMID: 28273854
    14. The results showed that the expression of TACC3 was downregulated in preeclamptic placentas. PMID: 27572091
    15. TACC3 overexpression was associated with clinicopathological features of aggressiveness, increased EMT-related protein expression, and poor survival, suggesting a potential role for TACC3 as a prognostic biomarker and therapeutic target in HCC PMID: 26219896
    16. Results showed that high level of TACC3 expression was correlated with advanced clinicopathological classifications, and poor prognosis in non-small cell lung cancer patients indicating that TACC3 is a potential prognostic marker. PMID: 26531241
    17. TACC3 is enriched in hepatocellular carcinoma and down-regulation inhibits the proliferation, clonogenicity, and cancer stem cell-like phenotype of HCC cells PMID: 26219398
    18. The measurement of TACC3 protein expression may be beneficial for predicting clinical outcomes for gastric cancer patients PMID: 26133271
    19. TACC3 depletion in human cell lines causes shorter mitotic spindles. co-immunoprecipitation experiments showed reduced binding between TACC3-F543A and Aurora-A. PMID: 26134678
    20. TACC3 is expressed in esophageal squamous cell carcinoma and correlates with poor prognosis PMID: 25760075
    21. FGFR3-TACC3 rearrangements occur in a subset of patients with lung adenocarcinoma. Such patients should be considered for clinical trials featuring FGFR inhibitors. PMID: 25294908
    22. our data demonstrated the oncogenic role of FGFR3-TACC3 in vitro, indicating that FGFR3-TACC3 may be useful as a diagnostic marker and therapeutic target in cancers. PMID: 25535896
    23. TACC3 depletion induces G1 arrest and cell death by activating p38-p53-p21 signaling and triggering a centrosome-mediated cellular stress response. PMID: 25613365
    24. findings suggest that TACC3 could be recruited as a bridge to cooperatively mediate between the HIF-2alpha PAS-B.ARNT PAS-B complex, thereby participating more directly in HIF-dependent gene transcription than previously anticipated PMID: 25627682
    25. findings provide critical information regarding the mechanisms by which TACC3 contributes to genomic instability PMID: 24769898
    26. TACC3 is involved in the regulation of microtubule nucleation at the centrosome and functions in the stabilization of the gamma-tubulin ring complex assembly PMID: 25246530
    27. TACC3 contributes to spindle formation in proliferating cancer cells. PMID: 24077290
    28. TACC3 phosphorylation by Aurora A kinase is required for central spindle assembly. PMID: 23887685
    29. Aurora-A kinase does not regulate TACC3-chTOG complex formation, indicating that Aurora-A solely functions as a recruitment factor for the TACC3-chTOG complex to centrosomes and proximal mitotic spindles. PMID: 24273164
    30. a novel function for TACC3 in EGF-mediated epithelial-mesenchymal transition in cervical cancer PMID: 23936413
    31. [review] TACC3 protein complexes are crucial for proper mitotic spindle assembly and dynamics to prevent faulty cell division and aneuploidy. PMID: 23787465
    32. Self-assembly and sorting of acentrosomal microtubules by TACC3 facilitate kinetochore capture during the mitotic spindle assembly. PMID: 24003142
    33. Identification of a novel oncogenic FGFR3-TACC3 fusion protein in bladder cancer. PMID: 23175443
    34. discovered FGFR3-TACC3 fusions in 4 of 48 glioblastoma samples, but not in any of 43 low-grade glioma samples tested. The fusion, caused by tandem duplication on 4p16.3, led to the loss of the 3'-UTR of FGFR3, blocking gene regulation of miR-99a. PMID: 23298836
    35. Identify TACC3 as a driver of tumorigenesis as well as an inducer of oncogenic epithelial-mesenchymal transition. PMID: 23348690
    36. study reports that a small subset of glioblastoma multiforme tumors harbors oncogenic chromosomal translocations that fuse in-frame the tyrosine kinase coding domains of fibroblast growth factor receptor genes(FGFR1 or FGFR3) to the transforming acidic coiled-coil coding domains of TACC1 or TACC3; the FGFR-TACC fusion protein displays oncogenic activity PMID: 22837387
    37. we have identified a novel submicroscopic duplication involving dosage sensitive genes TACC3, FGFR3, and LETM1. PMID: 21815251
    38. These findings link TACC3 to novel structural and cell division functions of TSC2. PMID: 20237422
    39. multifaceted genomic evaluation of glioblastoma establishes ERRFI1 as a potential candidate tumor suppressor gene and TACC3 as a potential oncogene, and provides insight on targets for oncogenic pathway-based therapy PMID: 21113414
    40. recruitment to spindle poles by clathrin ensures proper spindle assembly and chromosome alignment in mitotic cells through a RanGTP-dependent pathway PMID: 20923838
    41. Data show that ILK performs its centrosome clustering activity in a centrosome-dependent, manner through the microtubule regulating proteins TACC3 and ch-TOG. PMID: 20838383
    42. TACC3 recruits ch-TOG and clathrin to the mitotic spindle. Together the complex forms inter-microtubule bridges in kinetochore fibres. PMID: 21297582
    43. TACC3 controls paclitaxel sensitivity by modulating a premature senescence program. PMID: 20729911
    44. the association between aurora A phosphorylation and spindle apparatus; regulation from aurora A is mediated by CHC in recruiting phospho-TACC3 and subsequently ch-TOG to mitotic spindles. PMID: 20566684
    45. these findings suggest that Cdh1 controls TACC3 protein stability during mitotic exit. PMID: 19823035
    46. REVIEW: genetics, expression, gene expression regulation, and function studies PMID: 12389629
    47. TACC3 has an essential role in spindle assembly and cellular survival PMID: 17675670
    48. Aurora-A and TACC3 interaction is important to control the mitotic spindle organization required for proper chromosome segregation. PMID: 17914111
    49. overexpression of TACC3 may be associated with the mechanisms of chemoresistance, tumor progression, cell proliferation and metastasis PMID: 19148534

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole.
  • 蛋白家族：
    TACC family
  • 数据库链接：

    HGNC: 11524

    OMIM: 605303

    KEGG: hsa:10460

    STRING: 9606.ENSP00000326550

    UniGene: Hs.104019