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  4. STX4 Recombinant Monoclonal Antibody

STX4 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA290392A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, HL-60 whole cell lysate, A375 whole cell lysate, MCF-7 whole cell lysate
      All lanes: Syntaxin 4 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 35, 34 kDa
      Observed band size: 35 kDa
  • 其他：

产品详情

  • 产品描述：

    STX4 is a t-SNARE that is extensively expressed in numerous tissues, including skeletal muscle, and plays a vital role in glucose absorption in response to insulin in skeletal muscle and adipose tissue by delivering glucose transporter 4 (GLUT4) to the cell membrane. In beta cells, STX4 has also been demonstrated to modulate glucose-stimulated insulin production. It's also involved in cell invasion and invadopodium development, which is linked to the malignant growth of several human malignancies.

    The preparation of the recombinant STX4 antibody involves the mammalian cell lines expression of plasma vectors containing STX4 antibody genes. B cells isolated from immunized animals' blood were treated to obtain RNA, which underwent reverse transcription to yield DNA genes. Antibody genes were sequenced and screened from the DNA. After transient expression, cell supernatant was collected and then purified using Affinity-chromatography to obtain the recombinant STX4 antibody. This recombinant STX4 antibody is recommended to use in the STX4 for the detection of STX4 protein from Human.

  • Uniprot No.：
    Q12846
  • 基因名：
    STX4
  • 别名：
    Syntaxin-4 (Renal carcinoma antigen NY-REN-31), STX4, STX4A
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Syntaxin 4
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4E1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. In neurons, recruited at neurite tips to membrane domains rich in the phospholipid 1-oleoyl-2-palmitoyl-PC (OPPC) which promotes neurite tip surface expression of the dopamine transporter SLC6A3/DAT by facilitating fusion of SLC6A3-containing transport vesicles with the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes and in docking of synaptic vesicles at presynaptic active zones.
  • 基因功能参考文献：
    1. these results indicate that the activation of beta-ARs induces secretory granules and cell membrane fusion via the interaction of VAMP-2 and syntaxin-4 in a PKA- and F-actin-dependent manner in human submandibular gland. Up-regulated beta-ARs might participate in altering protein secretion in transplanted submandibular gland by promoting the interaction of VAMP-2 with syntaxin-4. PMID: 29358308
    2. Confocal immunofluorescence microscopy revealed a colocalization of syntaxin 4 with a MVB-specific marker, exosomes and hepatitis C virus (HCV) core, which suggests a fraction of syntaxin 4 is associated with exosomes loaded with HCV. Altogether, it is assumed that syntaxin 4 is a novel essential cellular factor for the release of HCV. PMID: 28624237
    3. The s found that activation of dendritic cells by bacterial lipopolysaccharide leads to increased Forster resonance energy transfer-fluorescence of fluorescently labeled syntaxin 4 with VAMP3 specifically at the plasma membrane, indicating increased SNARE complex formation, whereas FRET with other tested SNAREs was unaltered. PMID: 28524818
    4. Data suggest MUNC18C is required for STX4-mediated invadopodium formation and tumor invasion of extracellular matrix; N-terminal STX4 fragment binds to MUNC18C and inhibits interactions of STX4 with synaptosome-associated protein 23 (SNAP23) and vesicle-associated membrane protein 2 (VAMP2). Fibrosarcoma/adenocarcinoma cell lines were used in these studies. (MUNC18C = syntaxin binding protein MUNC18C; STX4 = syntaxin 4) PMID: 28798239
    5. The analysis revealed three candidate genes GSK3B, PTPN1, STX4 that are differentially expressed in study subjects. GSK3B was highly significant in Ps-T2D (P=0.00018, FR=-26.6), followed by Ps (P=0.0028, FR=-14.5) and T2D groups (P=0.032, FR=-5.9). PTPN1 showed significant association only with PS-T2D (P=0.00027, FR=-8.5). STX4 showed significant association with both Ps (P=0.0002, FR=-20) and Ps-T2D (P=0.0016, FR=-11.2). PMID: 27530212
    6. When the expression of STX4 mRNA was inhibited with short or small interfering, or silencing, RNA in macrophages, the survival of Brucella melitensis was significantly reduced. PMID: 25673907
    7. Syntaxin-4 has a role in mediating exocytosis of pre-docked and newcomer insulin granules underlying biphasic glucose-stimulated insulin secretion in human pancreatic beta cells PMID: 25762204
    8. Increased level of SNAP23-Syntaxin4-VAMP7 interaction correlates with decreased Syntaxin4 phosphorylation and trafficking of MT1-MMP to invadopodia during cellular invasion. PMID: 24807903
    9. upregulation of Syntaxin 4 is sufficient to significantly improve insulin secretory function to human type 2 diabetes islets retaining low levels of residual function PMID: 24552216
    10. STX4 is implicated in the antibody secretion. PMID: 24146186
    11. Syntaxin-4 plays a vital role in exocytosis of IgE from plasma cells. Knock-down of syntaxin-4, but not VAMP3 dramatically reduced IgE secretion from U266 plasma cells causing it to accumulate in the cell. PMID: 24055037
    12. These results revealed, for the first time, the extracellular role of syntaxin4 and shed light on the division of the extracellular effects exerted by epimorphin and syntaxin4 on keratinocyte cornification. PMID: 22226963
    13. Syntaxin 4 activation and insulin release in the absence of the glucose stimulus, consistent with nitrosative stress and dysfunctional exocytosis PMID: 21393240
    14. Stx4 is an essential postsynaptic component for synaptic plasticity in hippocampal neurons. PMID: 20959521
    15. Syntaxin 4 is required for acid sphingomyelinase activity and apoptotic function. PMID: 20956541
    16. dysferlin and syntaxin-4 similarly transit a common endosomal pathway in skeletal muscle cells. PMID: 20595382
    17. Stx4 defines an exocytic zone that directs membrane fusion for postsynaptic plasticity, revealing a novel specialization for local membrane traffic in dendritic spines. PMID: 20434989
    18. Syntaxin 4 and Synip (syntaxin 4 interacting protein) regulate insulin secretion in the pancreatic beta HC-9 cells PMID: 12855681
    19. syntaxin 4 is involved in the intracellular transport of MT1-MMP toward the plasma membrane PMID: 15351710

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  • 亚细胞定位：
    Cell membrane; Single-pass type IV membrane protein. Cell projection, neuron projection.
  • 蛋白家族：
    Syntaxin family
  • 组织特异性：
    Expressed in neutrophils and neutrophil-differentiated HL-60 cells. Expression in neutrophils increases with differentiation.
  • 数据库链接：

    HGNC: 11439

    OMIM: 186591

    KEGG: hsa:6810

    STRING: 9606.ENSP00000317714

    UniGene: Hs.83734