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  4. SPP1 Recombinant Monoclonal Antibody

SPP1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA261140A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 293T whole cell lysate, U-251 whole cell lysate, U-87 whole cell lysate
      All lanes: Osteopontin Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 36, 34, 33, 34, 34 kDa
      Observed band size: 36 kDa
    • IHC image of CSB-RA261140A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    SPP1, also called Osteopontin (OPN), is a multifunctional secreted integrin-binding glycol-phosphoprotein, that has been suggested to have a prognostic value and to be involved in the tamoxifen response. Overexpression of SPP1 has been found in various tumors, such as liver cancer, LC, prostate cancer (PCa), BC, and CRC. SPP1 exerts its effects by interacting with receptors that ultimately lead to tumor progression, invasion, and metastasis. High expression levels of SPP1 are associated with poor prognosis. Moreover, SPP1 was involved in tumor immunosuppression and influenced the tumor microenvironment.

    The main steps in the production of this SPP1 recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The SPP1 antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, WB, IHC.

  • Uniprot No.：
    P10451
  • 基因名：
  • 别名：
    Osteopontin (Bone sialoprotein 1) (Nephropontin) (Secreted phosphoprotein 1) (SPP-1) (Urinary stone protein) (Uropontin), SPP1, BNSP OPN
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Osteopontin
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    10A1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 基因功能参考文献：
    1. Plasma OPN levels were associated with the presence and severity of diabetic retinopathy in Asians with type 2 diabetes. PMID: 29463954
    2. Study demonstrated that osteopontin exerts an important role in the monocytes/macrophage phenotypic differentiation from hypertensive patients with vascular calcification. PMID: 28091516
    3. The expression of OPN was high in endometrium in secretory phase and in vitro decidualized endometrial stromal cells. Further analysis confirmed that OPN expression was upregulated by cAMP and C/EBPbeta signal pathway, while downregulated by miR181b. Increased OPN expression could promote the expression of decidualization-related and angiogenesis-related genes PMID: 29420252
    4. Results of the study showed that low urinary OPN levels were correlated with increased kidney stone risk, and dietary habits can affect urinary OPN level PMID: 30114399
    5. The serum levels of cystatin C and urine NGAL, urine OPN can be used as a good marker for assessing the renal effect of obesity which can lead end stage renal disease in pediatric population. PMID: 30035656
    6. novel 9175th- (exon 7) position polymorphism of OPN and rs17524488 were related to susceptibility to ankylosing spondylitis in a Chinese population PMID: 29581970
    7. Over-expressed and hypo-methylated SPP1 gene is associated with hepatocellular carcinoma. PMID: 27760737
    8. Serum osteopontin concentrations were increased in acute pancreatitis (AP) compared with follow-up 3 months after discharge. PMID: 29424811
    9. Our data provide evidence that leptin-mediated OPN upregulation promote TH2 inflammation in AR and this process is achieved through the alpha4 integrin and PI3K/AKT signaling pathways. PMID: 29885866
    10. miR-129-5p level was decreased in fibrotic liver of human, and reduced by rOPN treatment. In contrast, miR-129-5p was induced in HSCs transfected by OPN siRNA. These data suggested that OPN induces Col 1 expression via suppression of miR-129-5p in hepatic stellate cells. PMID: 29196165
    11. High circulating osteopontin levels predict major adverse cardiovascular events in patients with severe carotid artery stenosis. PMID: 29317141
    12. High SPP1 Expression is associated with Hepatocellular Carcinomas. PMID: 29693976
    13. Serum OPN and DKK1 levels of hepatocellular carcinoma patients could be considered as novel biomarkers showing prognostic significance after hepatectomy based on long-term survival data PMID: 29753515
    14. Upregulation of ADIPOR1 and SPP1, among the adipokine gene family, in cancer tissue is associated with poor survival in CRC, suggesting a potential mechanism linking obesity and colorectal cancer. PMID: 29761507
    15. Review/Meta-analysis: report a significantly higher circulating OPN level in systemic lupus erythematosus patients, a trend of positive correlation between OPN levels and SLE activity, and a significant association between OPN 1239 C/A and 9250 C/T polymorphisms, and SLE development. PMID: 27307447
    16. OPN is not only the pivotal protein controlling fibrosis, but may also serve as a biomarker associated with prognosis. PMID: 29120858
    17. Our data show that the ability of AML cells to constitutively release high levels of osteopontin is associated with a favorable prognosis, and this is different from previous studies of osteopontin mRNA levels. PMID: 27739925
    18. First report of OPN cleavage in THP-1 macrophages after PMA (phorbol 12-myristate 13-acetate) stimulation, and of enhanced cleavage induced by BCG infection (bovine tuberculosis). PMID: 29385060
    19. The results suggest that OPN, MMP9 and S100A8 all play a significant role in bladder cancer progression and are potential prognostic markers and therapeutic targets in bladder cancer PMID: 29209142
    20. We demonstrated an important effect of the SPP1 rs4754 on subclinical markers of carotid atherosclerosis in subjects with T2DM; however, as demonstrated by the multiple linear regression analysis, neither rs4754 nor rs28357094 had an important impact on the progression of subclinical markers of carotid atherosclerosis in subjects with T2DM. PMID: 28990744
    21. these results showed that expression of tear OPN protein by perennial allergic conjunctivitis patients increased significantly compared with controls or seasonal allergic conjunctivitis patients out of the pollen season PMID: 29279263
    22. The study revealed an enhanced sensitivity of aortic valve interstitial cells to osteogenic inductors in aortic stenosis patients, which indicates probable implication of OPN, OPG, and BMP2 genes in pathogenesis of aortic valve calcification. PMID: 29308559
    23. Carriers of the risky genotype or haplotype also had increased gastric OPN expression (p = 0.038) and inflammation (p = 0.007). SPP1 polymorphisms predispose to intestinal metaplasia development in H. pylori infected males. PMID: 28685609
    24. Respiratory syncytial virus infection leads to increased OPN expression.IL-1beta is involved in the regulation of OPN levels during respiratory syncytial virus infection. PMID: 29677209
    25. plasma level increases in pregnancy independently of asthma PMID: 29200898
    26. Methylglyoxal-bis-guanylhydrazone may have potential therapeutic utility in reducing or normalizing OPN levels and regulating monocyte activation in diseases that involve chronic inflammation. PMID: 29538412
    27. These results might be considered a useful starting point to validate OPN as a prognostic and diagnostic marker of hepatocellular carcinoma. PMID: 28711012
    28. study demonstrates that the OPN gene SNP rs1126616 is statistically associated with cerebral palsy. PMID: 27114095
    29. LAMP3 promotes the invasion of osteosarcoma cells via SPP1 signaling. PMID: 28849219
    30. Extended liver surgery is the only potentially curative treatment for patients with cholangiocarcinoma (CCA/biliary cancer), but it is currently unclear which patients benefit most from surgery. Detecting serum levels of osteopontin - a specific secreted glycoprotein involved in multiple diseases - in CCA patients might help to identify those patients that particularly benefit from tumour resection. PMID: 28668580
    31. MALAT1 could directly bind to miR-127-5p to inhibit its expression, so as to rescue OPN expression and promote chondrocyte proliferation through PI3K/Akt pathway. PMID: 28590075
    32. A linear negative correlation between serum OPN and total bone mineral density (BMD) of Type I diabetes mellitus patients compared to control group was noticed, indicating that the amount of serum OPN could be effective on BMD and a good predicting factor for osteoporosis PMID: 28499311
    33. OPN regulates CYP7A1 levels and the metabolic fate of liver acetyl-CoA as a result of CHOL and PC metabolism interplay PMID: 28754826
    34. these findings demonstrated that OPN and VEGF were overproduced in nasal polyps and that OPN induced VEGF production, which indicated that OPN-VEGF axis might contribute to angiogenesis in nasal polyps PMID: 28716167
    35. Osteopontin and CD44 play important roles in the development and progression of meningioma and can be used as prognostic markers for tumor recurrence and progression as well as therapeutic targets for the development of new drugs. PMID: 29504367
    36. OPN play a role in pathogenesis of neurodegenerative diseases or in neuroprotection by regulating the activation and function of microglia. PMID: 28698867
    37. osteopontin has a role in DNA repair and impact on human glioblastoma radiosensitivity PMID: 27563812
    38. SPP1 promoted the metastasis of CRC. PMID: 28531945
    39. Results show that osteopontin is positively associated with hepatocellular carcinoma (HCC) metastasis, and that OPN can activate CCR1 expression through the PI3K/AKT/HIF-1a signaling pathway to promote HCC progression and metastasis. PMID: 29285854
    40. This review presents the current knowledge on the expression profiles of OPN and its main splice variants in human cancers, as well as the potential implications in patient outcome. [review] PMID: 28440483
    41. Strong correlations between the expression of type I, II, IV collagen and osteopontin and the clinical stage of tympanosclerosis indicate the involvement of these proteins in excessive fibrosis and pathological remodeling of the tympanic membrane. PMID: 29068597
    42. result of this study confirms that increased levels of OPN exist in CSF and peripheral blood of MS patients and strengthens the evidence regarding the clinical utility of OPN as a promising and validated biomarker for MS PMID: 29346446
    43. OPN overexpression was correlated with poor overall survival and clinical features reflecting high aggressiveness in patients with gastric cancer. PMID: 27626167
    44. ITGbeta3 and CD44 expression levels determine whether OPN-a inhibits or enhances growth in lung cancer cells. PMID: 27487131
    45. Study identified that miR-127-5p targets the 3' UTR of osteoarthritis (OA) mRNA to down-regulate the expression of OPN. In OA, the down-expressed miR-127-5p allows the expression of OPN, which mediates the establishment and development of OA. PMID: 27126955
    46. Our study shows a strong effect of the rs28357094 G allele in increasing osteopontin expression in the presence of deflazacort, and adds to the evidence that rs28357094 polymorphism may predict response to glucocorticoids in Duchenne muscular dystrophy. PMID: 28595270
    47. we described the expression pattern of osteopontin splice variants in papillary thyroid carcinoma samples and the key role of osteopontin-a expression on activating tumor progression features PMID: 27409830
    48. In Osteoarthritis tissues, OPN mRNA, and NEAT1 expression was upregulated, whereas miR-181c expression was downregulated, indicating that targeting NEAT1 to rescue miR-181c expression so as to inhibit OPN expression and synoviocyte proliferation PMID: 28379604
    49. IL-6 and sIL-6R induced by IL-1beta may trigger IL-6 trans-signaling, contributing to the upregulation of OPN in THP-1 macrophages. Macrophages may be used as a source of IL-6 and sIL-6R and evoke IL-6 trans-signaling. PMID: 27863335
    50. OPN is a key mediator of intracerebral tumor growth, invasion, and dissemination in central nervous system lymphoma, and these effects depend upon activation of NF-kappaB. PMID: 27050077

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Osteopontin family
  • 组织特异性：
    Bone. Found in plasma.
  • 数据库链接：

    HGNC: 11255

    OMIM: 166490

    KEGG: hsa:6696

    STRING: 9606.ENSP00000378517

    UniGene: Hs.313