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  4. SAE1 Recombinant Monoclonal Antibody

SAE1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA987582A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate
      All lanes: SAE1 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 39, 30, 34 kDa
      Observed band size: 39 kDa
    • Immunofluorescence staining of Hela Cells with CSB-RA987582A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    SAE1 is an E-activating enzyme that plays a vital role in SUMOylation, a post-translational modification involved in cellular events such as regulation of transcription, cell cycle, and apoptosis. SAE1 forms a thioester bond between SUMO protein and UBA2/SAE2 by regulating the activation of ATP-dependent SUMO protein and then participates in the process of SUMO protein modification. SAE1 mainly mediates acetylation and phosphorylation and plays an important role in chromosome division and apoptosis. Upregulated expression of SAE1 has been implicated in the tumorigenesis and progression of several human malignancies, including glioma and gastric cancer.

    The main steps in the production of this SAE1 recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The SAE1 antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, WB, IF.

  • Uniprot No.：
    Q9UBE0
  • 基因名：
  • 别名：
    SUMO-activating enzyme subunit 1 (Ubiquitin-like 1-activating enzyme E1A) [Cleaved into: SUMO-activating enzyme subunit 1, N-terminally processed], SAE1, AOS1 SUA1 UBLE1A
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human SAE1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9D8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
  • 基因功能参考文献：
    1. the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is deregulated in the majority of PTC tissues, likely contributing to the PTC phenotype. PMID: 26403403
    2. as it has been shown by flow cytometry analysis, specific knockdown of SAE1 slowed down the cell population at G0/G1 phase and induced apoptosis of RKO cells. PMID: 25842831
    3. SUMO-1 controls the protein stability and the biological function of phosducin. PMID: 16421094
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Ubiquitin-activating E1 family
  • 组织特异性：
    Expression level increases during S phase and drops in G2 phase (at protein level).
  • 数据库链接：

    HGNC: 30660

    OMIM: 613294

    KEGG: hsa:10055

    STRING: 9606.ENSP00000270225

    UniGene: Hs.515500