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  4. RNF20 Recombinant Monoclonal Antibody

RNF20 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA227095A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate
      All lanes: RNF20 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 114 kDa
      Observed band size: 114 kDa
    • IHC image of CSB-RA227095A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA227095A0HU diluted at 1:100 and staining in paraffin-embedded human melanoma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela Cells with CSB-RA227095A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    The synthesized DNA sequence corresponding to the human RNF20 monoclonal antibody was cloned into the plasmid and then transfected into the cell line for expression. The product was purified through the affinity-chromatography method and obtained the RNF20 recombinant monoclonal antibody. This RNF20 recombinant antibody is a rabbit IgG and has been evaluated in ELISA, WB, IHC, and IF. It responds only with human RNF20.

    RNF20 belongs to the family of RING domain E3 ubiquitin ligases, which detect target substrates and transfer ubiquitin to them. RNF20 participates in a variety of biological processes, including transcription, cell division, and heat shock responses. RNF20 is somatically altered in breast, lung, prostate cancer, clear cell renal cell carcinoma (ccRCC), and mixed lineage leukemia, and its declined expression is a key factor in initiating genome instability. It also plays a role in oncogenesis as one of the major driving forces.

  • Uniprot No.：
    Q5VTR2
  • 基因名：
    RNF20
  • 别名：
    E3 ubiquitin-protein ligase BRE1A (BRE1-A) (hBRE1) (EC 2.3.2.27) (RING finger protein 20) (RING-type E3 ubiquitin transferase BRE1A), RNF20, BRE1A
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human RNF20
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    2H12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.
  • 基因功能参考文献：
    1. the RNF20/40 complex, a major ubiquitin ligase catalysing histone H2B monoubiquitination, interacts with the motor protein Eg5 during mitosis and participates in spindle assembly. PMID: 27557628
    2. data indicate that RNF20 and PARP1 are synthetic lethal interactors PMID: 28462496
    3. RNF20 depletion stabilizes the ZSCAN4 protein half-life, suggesting that RNF20 negatively regulates ZSCAN4 stability. PMID: 29477841
    4. The effects of RNF20 on mammary tumorigenesis are subtype dependent: In basal-like cancers cells, RNF20 suppresses the NF-kappaB-dependent expression of cytokines, known to contribute to the growth of this tumor subtype. PMID: 28157208
    5. The s also show that the RING domains of RNF20 and RNF40 can form a stable heterodimer that is active. PMID: 27569044
    6. Manipulation of key H2Bub1 E3 ubiquitin ligases, RNF20, RNF40 and BRCA1, in ovarian cancer cell line models modulated H2Bub1 levels, indicative of the role of these RING finger ligases in monoubiquitination of H2Bub1 in vitro PMID: 27798111
    7. RNF20 and H2Bub1 promotes chronic colonic inflammation and inflammation-associated colorectal cancer in mice and humans, partly by augmenting NF-kappaB activity and attenuating the antitumoral T cell response. PMID: 26854224
    8. It was observed that RE-IIBP induces MEIS1-mediated apoptosis, which was dependent on H2BK120 ubiquitination by RNF20. PMID: 26206755
    9. A primary role for FACT in RNF20 recruitment chromatin remodeling for initiation of homologous recombination repair. PMID: 24357716
    10. The ability of E1A to target hBre1 to simultaneously repress cellular IFN dependent transcription while activating viral transcription PMID: 23785282
    11. model whereby cotranscriptional recruitment of Rnf20 at MLL-fusion target genes leads to amplification of Dot1l-mediated H3K79 methylation, thereby rendering leukemia cells dependent on Rnf20 to maintain their oncogenic transcriptional program PMID: 23412334
    12. We show that Bre1 (human BRE1A/B (RNF20/40) and mouse Bre1a/b (Rnf20/40)) acts as an important suppressor of chromosomal instability PMID: 22354749
    13. our results suggest that RNF20 and RNF40, either via ubiquitylation of H2B or other targets, are coupled to the proliferation of prostate cancer cells. PMID: 22155569
    14. Studies indicate that H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40. PMID: 21827756
    15. RNF20, presumably via H2Bub, selectively represses oncogenic genes by interfering with chromatin recruitment of TFIIS, a factor capable of relieving stalled RNA polymerase II. RNF20 inhibits the interaction between TFIIS and the PAF1 complex. PMID: 21596312
    16. RNF20-mediated H2B ubiquitination at DSBs plays a critical role in HRR through chromatin remodeling PMID: 21362548
    17. the observed defects in the radiation response of Bre1a/b-deficient cells PMID: 20738173
    18. RNF20 overexpression leads to elevated histone 2B monoubiquitination, subsequently higher levels of methylation at H3 lysines 4 and 79, and stimulation of homeobox gene expression. PMID: 16307923
    19. The Bre1 protein specifically increases the global level of H2B ubiquitylation at lysine 120 and enhances activator-dependent transcription. PMID: 16337599
    20. identified as one of five genes containing 11 somatic mutations in a panel that included 132 colorectal cancers, then demonstrated that down-regulation of such homologs resulted in chromosomal instability and chromatid cohesion defects in human cells PMID: 18299561
    21. RNF20 suppresses the expression of several proto-oncogenes, which reside preferentially in closed chromatin and are modestly transcribed despite bearing marks usually associated with high transcription rates. PMID: 18832071
    22. hBre1 inhibits Ebp1's tumor suppressive activity through mediating its polyubiquitination and degradation. PMID: 19037095
    23. In humans, the 600 kDa RNF20/40 complex is the E3 ligase. PMID: 16307923
    24. Functional analysis of the yeast counterpart PMID: 12535538

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    BRE1 family
  • 组织特异性：
    Expressed in the normal brain and also in malignant gliomas (at protein level).
  • 数据库链接：

    HGNC: 10062

    OMIM: 607699

    KEGG: hsa:56254

    STRING: 9606.ENSP00000373772

    UniGene: Hs.729085