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RET Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA288083A0HU
  • 规格：
    ￥1320
  • 图片：
    • Overlay histogram showing Hela cells stained with CSB-RA288083A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1µg/1*106 cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    CUSABIO got the DNA sequence of the RET monoclonal antibody that was produced from the splenocytes generated by the human RET synthesized peptide immunization. The DNA sequence was cloned into the plasmid and then transfected into cell lines for in vitro expression. The product is the RET recombinant monoclonal antibody. It is a rabbit IgG antibody and is purified using the affinity-chromatography method. This RET antibody is recommended for ELISA and FC applications and detects the RET protein from human sources.

    RET is a proto-protein involved in many types of cancers. The high expression of RET in breast cancer makes it a potential therapeutic target for breast cancer. Minh Nguyen et al. demonstrated that two anti-RET antibody-drug conjugates (ADC) Y078-DM1 and Y078-DM4 displayed anti-tumor activity in pre-clinical models with breast cancer.

  • Uniprot No.：
    P07949
  • 基因名：
  • 别名：
    Proto-oncogene tyrosine-protein kinase receptor Ret (EC 2.7.10.1) (Cadherin family member 12) (Proto-oncogene c-Ret) [Cleaved into: Soluble RET kinase fragment, Extracellular cell-membrane anchored RET cadherin 120 kDa fragment], RET, CDHF12 CDHR16 PTC RET51
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Ret
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    8D12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, FC
  • 推荐稀释比：
    Application Recommended Dilution
    FC 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. Mediates, through interaction with GDF15-receptor GFRAL, GDF15-induced cell-signaling in the brainstem which induces inhibition of food-intake. Activates MAPK- and AKT-signaling pathways. Isoform 1 in complex with GFRAL induces higher activation of MAPK-signaling pathway than isoform 2 in complex with GFRAL.
  • 基因功能参考文献：
    1. mutation was found in 23.8% of hereditary medullary thyroid carcinoma patients tested; most commonly mutated codon was codon 634 (37.1%), followed by codon 918 (14.3%) PMID: 29779869
    2. Novel low frequency SNP in ERT locus is associated with Hirschsprung disease. PMID: 29379196
    3. RET alterations, such as RET-oncogene fusions, are present in a subset of breast cancers, and are promising therapeutic targets. PMID: 30446652
    4. RET gene alterations (copy number gain and rearrangement) exist in all RET-positive samples. RET-positive expression is a relatively independent factor in non-small cell lung cancer cases(NSCLC) patients, which indicates that the RET gene may be a novel target site for personalized treatment of NSCLC. PMID: 29473341
    5. Somatic mutations of the RET gene are underrecognized in HSCR. Molecular investigation of the parents of patients with seemingly sporadic mutations is essential to determine recurrence risk in these families. PMID: 29261189
    6. in vitro transactivation of the RET promoter by different Hirschsprung disease-associated PHOX2B polyA variants has resulted significantly lower compared to the effect of PHOX2B wild type protein. PMID: 28433712
    7. These results support the association between genetic variation of RET and NRG1 and susceptibility to Hirschsprung disease in the Chinese population. PMID: 28256518
    8. the results from three transcriptome-based platforms (Nanostring Elements, Agena LungFusion panel and ThermoFisher NGS fusion panel) were compared to those obtained from ALK, ROS1 and RET Fluorescence In Situ Hybridization on 51 clinical specimens. PMID: 28181564
    9. The s found a significant association between the localization of RET mutations and the expression of three genes: NNAT (suggested to be a tumour suppressor gene), CDC14B (involved in cell cycle control) and NTRK3 (tyrosine receptor kinase that undergoes rearrangement in papillary thyroid cancer) in patients with medullary thyroid cancer. PMID: 28181547
    10. the inverse relationship between GFRalpha1 and C-Ret, as knocking down C-Ret led to increases in GFRalpha1 expression. PMID: 29018141
    11. Rare synonymous changes in the RET gene, c.1827C>T (p.Cys609Cys), c.2364C>T (p.Ile788Ile), and c.2673G>A (p.Ser891Ser), were identified in medullary thyroid carcinoma patients and c.2418C>T (p.Tyr806Tyr) in a patient suspected of MEN2 syndrome PMID: 28647780
    12. RET rearrangement is associated with lung adenocarcinoma. PMID: 29549897
    13. The data suggest that all families with the C611Y germline mutation in Denmark originate from a recent common ancestor, probably explaining the unusually high prevalence of this mutation in Multiple Endocrine Neoplasia 2A families. PMID: 29020875
    14. Our results demonstrated greater expression of pRET and CXCR4 in cisplatinresistant neuroblastomas (NBs). Vandetanib significantly inhibited SHSY5YR cell proliferation, colony formation, and invasion, while downregulating pRET and CXCR4 expression PMID: 29436676
    15. LRIG1 is a negative regulator of RET2A and RET2B and is also downregulated in papillary and medullary thyroid carcinoma PMID: 29436694
    16. Study in SK-N-MC cells found that C634R mutation could enhance RET protein expression and change the location of the mutated protein and forced it into the nucleus. PMID: 29237911
    17. The frequencies of ALK, ROS1 and RET rearrangements are low in non-adenocarcinoma NSCLC patients. And their clinical characteristics are similar to those in lung adenocarcinoma. Fusions of the above 3 genes are not prognostic factor for non-adnocarcinoma NSCLC patients. PMID: 27635639
    18. BRAFV600E and RET/PTC and the expression of NF-kappaB promote the proliferation and migration of papillary thyroid carcinoma cells in vitro. PMID: 29117154
    19. The RET proto-oncogene located on chromosome 10q11.2 encodes a 1114-amino acid transmembrane receptor with a cadherin-related motif and a cysteine-rich domain in the extracellular domain. PMID: 28799054
    20. We found 6 single nucleotide polymorphisms in RET that were independent contributors to Hischsprung disease PMID: 28930629
    21. data establish differences in the mechanisms of RET9 and RET51 ubiquitylation and internalization that may influence the strength and duration of RET isoform signals and cellular outputs. PMID: 28794017
    22. Study demonstrate that the kinesin and kinase domains of KIF5B-RET act together to establish an emergent microtubule and RAB-vesicle-dependent RET-SRC-EGFR-FGFR signaling hub. Study demonstrate that drugs designed to inhibit RET alone work poorly in KIF5B-RET-transformed cells. PMID: 28877471
    23. RET knockdown significantly decreased xenografts tumor growth in vivo, confirming the oncogenic impact of RET signaling in vivo. PMID: 28490466
    24. Each of these autosomal dominant syndromes results from a specific germline mutation in unique genes: MEN1 is due to pathogenic MEN1 variants (11q13), MEN2A and MEN2B are due to pathogenic RET variants (10q11.21), MEN4 is due to pathogenic CDKN1B variants (12p13.1), and the HPT-JT syndrome is due to pathogenic CDC73 variants (1q25). PMID: 28674121
    25. RET p.C634F mutation is associated with Multiple Endocrine Neoplasia Type 2A with Cutaneous Lichen Amyloidosis. PMID: 29420094
    26. These data support the inclusion of patients bearing RET alterations in ongoing and future molecularly enriched clinical trials to explore RXDX-105 efficacy across a variety of tumor types. PMID: 28011461
    27. These results implicate EGFR as a key regulator of RET activation in A+AD and suggest that EGFR inhibitors may be therapeutic in patients with A+AD tumors even in the absence of an EGFR or RET mutation. PMID: 28460442
    28. In a cohort of MEN 2 families, the distribution of RET mutations in Denmark appears to differ from that of other populations. Mutations in codon 611 were the most prevalent, followed by more frequently reported mutations. This might be due to a possible founder effect for the p.C611Y mutation. PMID: 27809725
    29. RET inhibitors could both impair primary tumor growth and tumor dissemination, thereby providing a potential therapeutic advantage when used in combination with aromatase inhibitors in postmenopausal ER+ breast cancers. PMID: 27602955
    30. exposure of medullary thyroid cancer cells to a tri-substituted naphthalene diimide resulted in a significant antiproliferative activity paralleled by inhibition of RET expression PMID: 27351133
    31. Our data show that RET expression promotes a more mesenchymal phenotype with reduced cell-cell adhesion and increased invasiveness in papillary thyroid carcinoma cell models, but is more important for tumour cell survival, proliferation and anoikis resistance in medullary thyroid carcinoma models. Our data suggest that the RET51 isoform plays a more prominent role in mediating these processes compared to RET9. PMID: 27872141
    32. From this case series, the largest such experience to date, it is concluded that the RET(K666N) variant is likely pathogenic and associated with low penetrance of medullary thyroid carcinoma. PMID: 27673361
    33. Multilayer OMIC data analysis uncovered methylation hallmarks in genetically defined Medullary thyroid carcinoma (MTC) and revealed JAK/Stat signaling effector STAT3 as a potential therapeutic target for the treatment of RET(M918T) MTCs PMID: 27620278
    34. DNA mutational analysis of RET germline mutations associated with medullary thyroid carcinoma in a Druze family. PMID: 28688347
    35. increased overall survival was observed in breast cancer patients who are carriers of the variant allele of SNP rs2435357 PMID: 27034161
    36. These data suggest that angiogenesis in RET mutation medullary thyroid carcinomas may be more intense and complete than that found in RETwt tumors, a feature that might increase their susceptibility to antiangiogenic therapy. PMID: 27402614
    37. Significant genetic risk for Hirschsprung disease (HSCR) was imparted by rs2435357 and rs2506030 at RET and by rs12707682 at SEMA3 in a Chinese population. No evidence was found of a genetic association between HSCR and either of the NRG1 SNPs rs7835688 and rs16879552, at either allele or genotype level. PMID: 27203398
    38. Durable benefits with pemetrexed-based therapies in RET-rearranged lung cancers are comparable with ALK- and ROS1-rearranged lung cancers. When selecting therapies for patients with RET-rearranged lung cancers, pemetrexed-containing regimens should be considered. PMID: 27056998
    39. Data suggest that the KIAA1217-RET-fusion gene is a promising target for lung cancer treatment. PMID: 27150058
    40. The RET M918V mutation is co-segregating in 8 familial MTC kindreds with validated evidence of a founder effect. PMID: 27807060
    41. Mutational analysis in 17 cases of Medullary thyroid carcinoma, the somatic missense mutation at codon 918 of RET was found in 2 of the 17 Medullary thyroid carcinoma(MTC)s, and one case presented MEN2 phenotype including MTC. PMID: 28166591
    42. study provided useful information on RET variants that should be subjected to further study PMID: 29131865
    43. Review of RET mutations and mechanisms in medullary thyroid cancer. PMID: 26678667
    44. The cardiac GFRA2 signaling pathway is distinct from the canonical pathway dependent on the RET tyrosine kinase. PMID: 27396331
    45. identified four genomic rearrangements involving the genes BRAF, RET, and ROS1 PMID: 27864876
    46. Detection of Gene Rearrangements in Circulating Tumor Cells: Examples of ALK-, ROS1-, RET-Rearrangements in Non-Small-Cell Lung Cancer and ERG-Rearrangements in Prostate Cancer.( PMID: 28560674
    47. These findings support the role of RET in the development of the enteric nervous system but underline the importance of other genetic or environmental factors contributing to the gastrointestinal phenotype of the disease. Somehow, this RET R114H mutation proved to have a role in the etiology of both CIPO and HSCR and could contribute to a more diffuse imbalance of gut dysmotility. PMID: 27273837
    48. High RET expression is associated with perineurial invasion of pancreatic adenocarcinoma. PMID: 28092668
    49. RET expression was significantly greater in patients with Extraskeletal myxoid chondrosarcoma relative to other types of sarcomas except for liposarcoma PMID: 28423517
    50. RET gene rearrangement plays a role in the pathogenesis of papillary thyroid cancer. PMID: 28911147

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  • 相关疾病：
    Colorectal cancer (CRC); Hirschsprung disease 1 (HSCR1); Medullary thyroid carcinoma (MTC); Multiple neoplasia 2B (MEN2B); Pheochromocytoma (PCC); Multiple neoplasia 2A (MEN2A); Congenital central hypoventilation syndrome (CCHS)
  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family
  • 数据库链接：

    HGNC: 9967

    OMIM: 114500

    KEGG: hsa:5979

    STRING: 9606.ENSP00000347942

    UniGene: Hs.350321