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  4. RBP4 Recombinant Monoclonal Antibody

RBP4 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA784971A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA784971A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA784971A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    RBP4 belongs to the lipocalin family and is the primary transport protein in the circulation for the hydrophobic chemical retinol (vitamin A). It is mainly synthesized and secreted in the liver. RBP4 transports retinol from the liver to target tissues and makes up an important regulator of retinol levels in the bloodstream. RBP4 has been linked to a number of human disorders, including vision loss and ocular ailments, as well as type 2 diabetes. In mice and humans, RBP4 has been demonstrated to operate as an adipokine implicated in insulin sensitivity modulation. RBP4 also acts as a hepatokine in mice, mediating the effects of the molecular clock on glucose metabolism.

    The recombinant RBP4 antibody expression is induced in mammalian cells transfected with a recombinant plasma vector. The recombinant plasma vector was constructed by inserting the gene coding for the antibody against RBP4 into the plasma. The recombinant RBP4 antibody was purified from the cell culture medium using Affinity-chromatography. It can react with samples containing RBP4 protein from Human and has been validated for use in the ELISA, IHC.

  • Uniprot No.：
    P02753
  • 基因名：
  • 别名：
    Retinol-binding protein 4 (Plasma retinol-binding protein) (PRBP) (RBP) [Cleaved into: Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176)], RBP4
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human RBP4
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9G7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Retinol-binding protein that mediates retinol transport in blood plasma. Delivers retinol from the liver stores to the peripheral tissues. Transfers the bound all-trans retinol to STRA6, that then facilitates retinol transport across the cell membrane.
  • 基因功能参考文献：
    1. Baseline RBP4 levels and MELD scores predicted 21-day (/=25) mortality, respectively. In critically ill patients with underlying liver disease, with a link to eGFRs, INRs and TC levels, the baseline RBP4 may serve as a marker for short-term mortality. PMID: 28588245
    2. our study provides clinical evidence revealing that the serum concentrations of RBP4 were elevated in NAFLD patients in a Chinese population. These findings indicated that RBP4 might be a noninvasive molecular biomarker that detects the presence of NAFLD in middle-aged and elderly population. PMID: 28332619
    3. Vitreous levels of APOA1 and RBP4 in human rhegmatogenous retinal detachment associated with choroidal detachment reflects the severity of disease. PMID: 29618920
    4. The overexpression of RBP4 increased cell proliferation, whereas siRNA-mediated RBP4 knockdown significantly decreased HTR8/SVneo cell proliferation via activation of PI3K/AKT signaling. PMID: 30015949
    5. High RBP4 expression is associated with hypertriglyceridemia. PMID: 29747616
    6. We observed that knockdown of RBP4 can greatly suppress ovarian cancer cell migration and proliferation. PMID: 29642915
    7. This study showed that the Levels of circulating RBP4 was significantly higher in Chronic Kidney Disease than in control. PMID: 29678848
    8. Our results demonstrate that IR is associated with high circulating RBP4 and that suppressed RBP4 adipose tissue expression is accompanied by reduced GLUT4 expression in HD. Renal transplantation or HDF are effective in lowering serum RBP4 levels. PMID: 29333450
    9. Data suggest that serum levels of RBP4 and LGAL3BP are up-regulated after menopause when complicated by NAFLD (non-alcoholic fatty liver disease); RBP4 and LGAL3BP may serve as biomarkers of NAFLD in postmenopausal women. (RBP4 = retinol binding protein 4; LGAL3BP = galectin-3 binding protein) PMID: 29679552
    10. Elevated levels of RBP4 were not associated with an increased risk of ischemic stroke among women. PMID: 28888344
    11. Study showed that dietary weight loss-induced changes in angiotensin-converting enzyme activity, free fatty acids and RBP4 independently contribute to weight regain prediction. PMID: 29122953
    12. RBP4 is involved in all-trans retinoic acid-induced cleft palate. PMID: 28849085
    13. the 1.5A resolution structures of human holo-RBP4 and of the protein saturated with palmitic and lauric acid and discuss the interaction of the fatty acids and retinol with the protein, are reported. PMID: 29414511
    14. Circulating RBP4 levels may not be associated with nonalcoholic fatty liver disease. PMID: 28931435
    15. Plasma PEDF and RBP4 identified insulin resistance in subjects with no prior diagnosis of diabetes. PMID: 28648555
    16. Serum RBP4 level was significantly higher and closely associated with blood pressure in prehypertensive Chinese. PMID: 28639612
    17. Conclusion RBP4 may be used as a predictive factor of diabetic nephropathy patients complicated with silent cerebral infarction (SCI) and is positively correlated with cognitive dysfunction. RBP4/Lp-PLA2/Netrin-1 pathway activation may be one of the occurrence mechanisms in diabetic nephropathy complicated with SCI. PMID: 28704853
    18. these data demonstrate a key role of STRA6 and RBP4 in the maintenance of colon cancer self-renewal and that this pathway is an important link through which consumption of HFD contributes to colon carcinogenesis. PMID: 28689994
    19. Data suggest that, in subjects with chronic kidney disease, renal function is inversely related to serum RBP4 levels; as GFR decreases, the relationship between RBP4 and insulin resistance is attenuated. (GFR = glomerular filtration rate, a measure of kidney function) PMID: 28473187
    20. Results identified RBP4 as a promising acute liver failure (ALF) biomarker among energy metabolism-related proteins. RBP4 values signaled liver injury at an early stage, as its decreasing level was observed before ALT elevation in the pig ALF model, and its significant decreasing level in ALF patients. PMID: 28336726
    21. Elevated RBP4 is detectable early in pregnancy and has strong correlation with preeclampsia and premature birth. PMID: 28338737
    22. RBP4-induced inflammation is largely mediated by TLR4. PMID: 28400700
    23. RBP4 may be associated with higher cardiovascular risk in overweight/obese adolescent girls, but this association is mediated by abdominal obesity. PMID: 29120147
    24. Bi-allelic mutations in RBP4 were identified (c.248+1G>A), consistent with a diagnosis of inherited vitamin A deficiency. PMID: 27892788
    25. Childhood obesity may be associated with variations in RBP4 gene. The presence of selective SNPs in the RBP4 gene may account for metabolic complications. PMID: 26611784
    26. Low levels of vitamin A, E and RBP4 at the time of renal cell carcinoma diagnosis are associated with a poorer prognosis after surgery. PMID: 28668878
    27. RBP4 is some 6-fold lower when active TB patients have chronic energy deficiency (CED) than when BMI is >25 kg/m sq; however, free fatty acids were not associated with CED in active TB patients, which may be a type 2 error or represent an energy impasse where infection and the host's metabolic needs are in competition. PMID: 28625041
    28. Data show that the tissue-specific expression pattern of human transgenic retinol binding protein 4 (hRBP4orf) was roughly the same as that of mouse Rbp4. PMID: 28134916
    29. Elevated levels of RBP4 are associated with higher cardiovascular mortality among men with type 2 diabetes. PMID: 27609367
    30. RBP4 and retinol levels were increased approximately twofold in patients with chronic kidney disease. PMID: 27277845
    31. RBP4 level was associated with increased arterial stiffness in young subjects with family history of type 2 diabetes. PMID: 26868132
    32. Retinol-binding protein 4 is able to function as biomarker to distinguish severe pre-eclampsia from normal pregnancy. More importantly, these results may shed light on the role of Retinol-binding protein 4 in the pathogenesis in pre-eclampsia. PMID: 27279411
    33. High level of RBP4 is associated with obesity. PMID: 26794633
    34. The positive association between circulating RBP4 and ApoB-containing lipoproteins in a steady metabolic state, as well as during a hypocaloric diet, appears to be attenuated in patients with very high serum triglycerides. PMID: 27086684
    35. Urinary KNG1 and RBP4 clearly respond to AKI. Additionally, RBP4 could predict recovery by monitoring this protein levels over time after AKI, as RBP4 reflects patient's normalization earlier than sCr values do. PMID: 26792617
    36. SNPsRBP4 was associated with age at onset in familial amyloid polyneuropathy. PMID: 26286643
    37. Serum RBP4 is negatively related to estrogen in Chinese women with obesity. PMID: 26960804
    38. Dietary vitamin A may reduce abdominal adiposity and promote visceral to subcutaneous body fat redistribution during adolescence in an RBP4-dependent manner. PMID: 26667887
    39. The results of this meta-analysis support the hypothesis that RBP4 is a modest independent risk factor for gestational diabetes mellitus (i.e., nonobese patients with gestational diabetes mellitus might express RBP4 at abnormal levels).The association between RBP4 rs3758539 polymorphism and gestational diabetes mellitus risk was not confirmed. PMID: 26975349
    40. RBP-4 levels are higher in patients with clinical hypothyroidism and exhibit a marked decrease after normalization of thyroid function in both hyper and hypothyroid patients. We suggest that RBP-4 may play a role in the metabolic disturbances which accompany thyroid dysfunction. PMID: 26575118
    41. Fasting concentrations of RBP-4 were negatively correlated with BMI and waist-hip ratio, whereas lipocalin-2 levels were positively associated with BMI and waist-hip ratio. PMID: 26069091
    42. male mice are susceptible to high fat diet-induced hyperglycaemia and display higher plasma RBP4 levels, possibly due to its over-expression in visceral adipose depots PMID: 26619134
    43. RBP4 concentrations were significantly lower in hepatitis C subjects compared to controls. PMID: 26927700
    44. Presence of gestational diabetes mellitus risk factors does not have an impact on early second trimester RBP-4 values in pregnant subjects. PMID: 25227411
    45. This study describe the discovery and subsequent replications of RBP4 and its combination with circulating GFAP as plasmatic biomarkers for hyperacute stroke subtype differentiation. PMID: 26526443
    46. Gc1s/Gc1s phenotype variant of DBP and the unbound fraction of plasma RBP4 may be considered as factors related with the incidence, and possibly the risk, of IR in CHC patients. PMID: 26962819
    47. RBP correlated with IgG4 and IgA. RBP also correlated positively with age and with influenza virus-specific antibody neutralization titres. PMID: 26425827
    48. Genetic variants of retinol-binding protein 4 in adolescents are associated with liver function and inflammatory markers but not with obesity and insulin resistance PMID: 26440092
    49. Baseline serum RBP4 levels were independently associated with incident diabetes in Asian Indian men with impaired glucose tolerance. PMID: 25810022
    50. Findings suggested that Asian women with gestational diabetes mellitus had increased circulating RBP4 levels in their second/third trimester. [Meta-Analysis] PMID: 25703255

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  • 相关疾病：
    Retinal dystrophy, iris coloboma, and comedogenic acne syndrome (RDCCAS); Microphthalmia, isolated, with coloboma, 10 (MCOPCB10)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Detected in blood plasma and in urine (at protein level).
  • 数据库链接：

    HGNC: 9922

    OMIM: 180250

    KEGG: hsa:5950

    STRING: 9606.ENSP00000360519

    UniGene: Hs.50223