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  4. RBBP4 Recombinant Monoclonal Antibody

RBBP4 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA915915A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate
      All lanes: RbAp48 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 48, 48, 47, 44 kDa
      Observed band size: 53, 40 kDa
    • IHC image of CSB-RA915915A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA915915A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela Cells with CSB-RA915915A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    RBBP4 contributes to the repression of gene transcription as a key member of the nucleosome remodeling and deacetylation (NURD) complex, polycomb repressor complex 2 (PRC2), and SIN3- chromatin-modulating complexes. As a member of the chromatin assembly factor 1 (CAF1) complex, RBBP4 regulates chromatin assembly in normal replication and during the repair of DNA damage. RBBP4 interacts with ep300 protein to form a complex that drives the expression of methylguanine-DNA-methyltransferase , RAD51, and other selected DNA repair genes through histone acetylation.

    The main production processes of this recombinant RBBP4 antibody included immunization, B cell harvest, antibody secreting cell enrichment, single cell sequencing, antibody expression and purification. The single B cell screening platform was used for the RBBP4 antibody gene screening. And this RBBP4 antibody was tested in ELISA, WB, IHC, IF.

  • Uniprot No.：
    Q09028
  • 基因名：
  • 别名：
    Histone-binding protein RBBP4 (Chromatin assembly factor 1 subunit C) (CAF-1 subunit C) (Chromatin assembly factor I p48 subunit) (CAF-I 48 kDa subunit) (CAF-I p48) (Nucleosome-remodeling factor subunit RBAP48) (Retinoblastoma-binding protein 4) (RBBP-4) (Retinoblastoma-binding protein p48), RBBP4, RBAP48
  • 反应种属：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide derived from human RbAp48
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3E9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
  • 基因功能参考文献：
    1. ARMC12 promotes neuroblastoma progression through interaction with RBBP4. PMID: 30026490
    2. BCL11A interacts with histone methyltransferase (PRC2) and histone deacetylase (NuRD and SIN3A) complexes through their common subunit, RBBP4/7. PMID: 29263092
    3. RBAP48 overexpression contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3-kinase/protein kinase B pathway suppression. PMID: 29901205
    4. The MTA1 subunit of the nucleosome remodeling and deacetylase complex can recruit two copies of RBBP4/RBBP7. PMID: 27144666
    5. RbAp48 is likely to act as a potent antiretroviral defense. PMID: 27222146
    6. The crystal structure reveals an extensive interface between MTA1 and RBBP4. PMID: 27098840
    7. RBBP4 interacts with ep300 protein to form a complex that drives the expression of methylguanine-DNA-methyltransferase , RAD51, and other selected DNA repair genes through histone acetylation. PMID: 26972001
    8. RbAp48 was identified as critical in the proliferation of hypopharyngeal carcinoma in both in vitro and in vivo experiments. PMID: 26376479
    9. RBBP4 functions as a novel regulatory factor to increase the efficiency of importin alpha/beta-mediated nuclear import PMID: 26491019
    10. It is associated with episodic memory performance. PMID: 25150575
    11. Our RBBP4-PHF6 complex structure provides insights into the molecular basis of PHF6-NuRD complex interaction and implicates a role for PHF6 in chromatin structure modulation and gene regulation. PMID: 25601084
    12. Our results reveal that the protein structure does not affect ligand binding, and the top three TCM candidates Bittersweet alkaloid II, Eicosandioic acid, and Perivine might resolve the instability of the RbAp48-FOG1 complex PMID: 25165715
    13. RbAp48 recognizes MTA1 using the same site that it uses to bind histone H4, showing that assembly into NuRD modulates RbAp46/48 interactions with histones. PMID: 24920672
    14. The most significant change was an age-related decline in RbAp48, a histone-binding protein that modifies histone acetylation. PMID: 23986399
    15. study of the interaction of the histone H3-H4 complex with the RbAp48 and their exchange with a second histone chaperone, anti-silencing function protein 1 (ASF1); exchange of histones H3-H4 between these two histone chaperones has a central role in the assembly of new nucleosomes PMID: 23178455
    16. Data identified RBBP4 and RBBP9 as required for maintenance of multiple PS cell types, and both RBBPs were bound to RB in PS cells. PMID: 21689726
    17. The FOG-1 peptide contacts a negatively charged binding pocket on top of the RbAp48 beta-propeller that is distinct from the binding surface used by RpAp48 to contact histone H4 PMID: 21047798
    18. Upergulation of CAF-1 p48 subunit by overexpression of tuberous sclerosis gene products. PMID: 12893285
    19. expression of three (Beclin 1, RbAp48 and Pir51) were increased and one (aldolase b) was decreased in liver tumor tissues. PMID: 14966907
    20. RbAp48 is a NF-kappaB-regulated gene playing an important role in thyroid cancer cell autonomous proliferation. PMID: 17244783
    21. In conclusion, the data support a model whereby RbAp48 regulates cellular morphology and cytoskeletal organization by increasing K-Ras activity and signaling through MAPK. PMID: 17974974
    22. Data show that RbAp46 and RbAp48 interact with estrogen receptor (ER)alpha at endogenous, estrogen-responsive genes and alter expression of endogenous, ERalpha-activated and -repressed genes in MCF-7 breast cancer cells. PMID: 18577416
    23. Results report that transgenic expression of RbAp48 resulted in the development of autoimmune exocrinopathy resembling Sjogren's syndrome. PMID: 19015307

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WD repeat RBAP46/RBAP48/MSI1 family
  • 数据库链接：

    HGNC: 9887

    OMIM: 602923

    KEGG: hsa:5928

    STRING: 9606.ENSP00000362592

    UniGene: Hs.16003