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Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221) Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA013466A183phHU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in 293 whole cell lysate(treated with EGF or not)
      All lanes Phospho-MAPK8/MAPK9/MAPK10 antibody at 1.65μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 46,54 KDa
      Observed band size: 46,54 KDa
    • Immunocytochemistry analysis of CSB-RA013466A183phHU diluted at 1:165 and staining in Hela cells(treated with 100ng/ml EGF for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • Uniprot No.：
    P45983 P45984 P53779
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-MAPK8/MAPK9/MAPK10 (T183/T183/T221)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1A9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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