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  4. Phospho-GATA3 (S308) Recombinant Monoclonal Antibody

Phospho-GATA3 (S308) Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA009276A308phHU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in Hela whole cell lysate,A549 whole cell lysate
      All lanes Phospho-GATA3 antibody at 2.5μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 48 KDa
      Observed band size: 48 KDa
    • IHC image of CSB-RA009276A308phHU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品描述：

    CUSABIO put the phospho-GATA3 (S308) monoclonal antibody DNA sequence into the plasmid, which was subsequently transfected into the cell line for expression. Immunizing mice with the synthetic phospho-peptide corresponding to residues surrounding Ser 308 of human GATA3 produced the phospho-GATA3 (S308) monoclonal antibody. The recombinant phospho-GATA3 (S308) monoclonal antibody was obtained after the product was purified using affinity chromatography. It's a rabbit IgG antibody. This phospho-GATA3 (S308) antibody has undergone ELISA, WB, and IHC quality testings. It is reactive with human samples. This anti-pSer308-GATA3 antibody may be used to address the functional role of GATA3 phosphorylation.

    GATA3 is mostly expressed in T lymphocytes and is essential for both early thymic T-cell development and functional differentiation of naive CD4 T cells into Th2 cells. GATA3, a T-cell transcription factor, has been found to influence Th2 T-cell development in a number of investigations. It also plays a role in mammary gland development and the preservation of luminal epithelial cells' differentiated state. Posttranslational modifications, the proteasome pathway, and phosphorylation all can regulate GATA3 activity. In ER-positive breast cancer cells, phosphorylation of GATA3 at Ser308 has previously been utilized as a marker of proteasomal turnover.

  • Uniprot No.：
    P23771
  • 基因名：
  • 别名：
    GATA 3 antibody; GATA binding factor 3 antibody; GATA binding protein 3 antibody; GATA-binding factor 3 antibody; Gata3 antibody; GATA3_HUMAN antibody; HDR antibody; HDRS antibody; MGC2346 antibody; MGC5199 antibody; MGC5445 antibody; Trans acting T cell specific transcription factor GATA 3 antibody; Trans-acting T-cell-specific transcription factor GATA-3 antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-GATA3 (S308)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1E5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression.
  • 基因功能参考文献：
    1. The data indicate that expression of CRTh2 is regulated through the competitive action of GATA3 and NFAT1. PMID: 29969451
    2. the present study postulates that the genetic variation of the transcription factor GATA3, not STAT4, is associated with the risk of type 2 diabetes in the Bangladeshi population. PMID: 30044774
    3. one allele of the GATA3 second zinc finger leads to loss of binding and decreased expression at a subset of genes, including Progesterone Receptor PMID: 29535312
    4. Study established an important role of p38gamma MAPK in epithelial-mesenchymal transition (EMT) and identified a novel signaling pathway for p38gamma MAPK-mediated tumor promotion. p38gamma MAPK regulated miR-200b through inhibiting GATA3 by inducing its ubiquitination, leading proteasome-dependent degradation. PMID: 30251680
    5. Treg cells from asthmatic patients expressed more FOXP3 as well as GATA3; the expression level of GATA3 negatively correlated with FEV1%pred. Increased expressions of USP21 and PIM2 in Treg cells from asthmatic patients were found. PMID: 30013989
    6. GATA 3 is more sensitive marker than mammaglobin and GCDFP-15 for diagnosing metastatic breast carcinoma in cytological cell block materials PMID: 29235613
    7. This study demonstrates consistent GATA-3 staining in walthard nests, rare staining in adenomatoid tumors, and is otherwise rarely positive in normal urologic and gynecologic mesothelia. Furthermore, GATA-3 is uniformly positive in epididymi and negative in efferent ductules which may reflect the embryological evolvement of these tissues. PMID: 28582342
    8. GATA3 staining to differentiate primary cutaneous apocrine cribriform carcinoma from skin breast cancer metastasis has a high negative predictive value PMID: 29431200
    9. GATA3 mutations, recently observed in breast cancer, encode active transcription factors. PMID: 30061207
    10. KMT1A positively regulated the self-renewal and tumorigenicity of human Bladder cancer stem cells via KMT1A-GATA3-STAT3 circuit, in which KMT1A could be a promising target for bladder cancer therapy. PMID: 28765327
    11. Defective sirtuin-1 was found to increase IL-4 expression through acetylation of GATA-3 in patients with severe asthma compared with healthy controls. PMID: 26627546
    12. GATA3 activation was diminished upon cultivation of T cells with RNase 7. PMID: 28378334
    13. overexpression of GATA3 and FOXA1 cooperate with PPAR activation to drive transdifferentiation of a basal bladder cancer cells to a luminial phenotype. PMID: 27924948
    14. The data have demonstrated that GATA3 is by far the most reliable breast-specific immunomarker in both surgical and cytological specimens PMID: 28965624
    15. GATA3 interacts with and is acetylated by the acetyltransferase CBP. The major acetylated site of GATA3 in lung adenocarcinoma cells is lysine 119. PMID: 29453984
    16. GATA3 may be useful as a part of a panel of immunohistochemical markers in narrowing the differential diagnosis between pheochromocytoma and adrenal cortical carcinoma PMID: 28374498
    17. s demonstrated that ER(alpha), GATA3 and FOXA1 form a transcriptional complex with Ell3 to regulate IL-20 expression in ER(+) breast cancer cells. FOXA1 represses IL-20 expression, whereas GATA3 and ER(alpha) activate it. PMID: 28514748
    18. We have identified ZPO2 as a negative regulator of GATA3, thus providing an alternative mechanism that results in a reduction in or perhaps even loss of GATA3 during breast cancer development. PMID: 28258171
    19. Our study has demonstrated that GATA3 expression is common in primary triple-negative breast carcinomas PMID: 28211079
    20. GATA3 is a sensitive marker for primary genital extramammary paget disease PMID: 28693610
    21. Benign prostate glands with radiation atypia show diffuse positivity for GATA3 PMID: 28316088
    22. In summary, we documented a transcriptional regulation of GATA3 in glioblastoma cells upon transfection with GAB sequence. PMID: 28614770
    23. Results showed that GATA3 along with TRPS1 are distinctively overexpressed in breast cancer (BC) among all GATA family members and predict better survival in patients with BC. GATA3 is then a distinctive biomarker and essential prognostic factor in BC. PMID: 28423734
    24. s found a high sensitivity for all the markers analyzed. Moreover, the expression of NY-BR-1 and GATA-3 seemed the most effective for labelling male breast cancer in primary and metastatic setting. PMID: 29116378
    25. These results suggest that high GATA3 expression is a predictor of a poor prognosis in peripheral T cell lymphoma, and that T lymphoma cells promote M2-type macrophage differentiation through a GATA3-dependent mechanism. PMID: 27589565
    26. We demonstrate that engagement of the T-cell receptor (TCR) on malignant T cells culminates in NF-kappaB activation and the upregulation of GATA-3 expression, both of which regulate the growth and survival of conventional T cells, and were shown to promote chemotherapy resistance in malignant T cells PMID: 27780854
    27. These results suggest that the ELK3-GATA3 axis is a major pathway that promotes metastasis of breast cancer MDA-MB-231 cells PMID: 27556500
    28. As a transcriptionally regulated program, urothelial differentiation operates as a heterarchy, wherein GATA3 is able to co-operate with FOXA1 to drive expression of luminal marker genes, but that P63 has potential to transrepress expression of the same genes. PMID: 28282036
    29. GATA-3 in lymphocytes showed the increased expression of GATA-3 in elderly people in comparison with the young. Women demonstrated higher expression of GATA-3 in compare with men. PMID: 28509479
    30. C-MYC is related to GATA3 and Ki-67 expression and is associated with poor prognosis in nodal peripheral T-cell lymphomas PMID: 27151990
    31. The GATA3 rs3824662 A allele and AA genotype may be risk factors for the development of pediatric ALL especially B-ALL in the studied cohort of Egyptian patients. The AA genotype is associated with shorter DSF, increased incidence of relapse and poor prognosis in pediatric ALL. PMID: 27684731
    32. Mutation in the GATA-3 gene is associated with hypoparathyroidism, deafness and renal dysplasia syndrome. PMID: 27387476
    33. GATA3 is a reliable diagnostic marker for Neuroblastomas not only in scant/limited surgical specimens but also in cytologic samples, including air-dried touch imprints, which have previously been undescribed for this marker. PMID: 28976719
    34. Dysregulation of JAM-A via p63/GATA-3 signaling pathway occurs in squamous cell carcinomas of the head and neck. PMID: 27036044
    35. FBXW7alpha inhibits breast cancer cells survival by promoting GATA3 degradation/destabilizing GATA3. PMID: 28722108
    36. GATA3-driven expression of miR-503 inhibits prostate cancer progression by repressing ZNF217 expression. PMID: 27267060
    37. MIR-720 suppresses M2 macrophage polarization by targeting GATA3; MIR-720 is down-regulated in breast cancer macrophages. PMID: 27354564
    38. the model of mutually antagonistic differentiation programs driven by mutually exclusively expressed T-bet or GATA-3 does not completely explain natural CD4 T cell priming outcomes PMID: 29088218
    39. GATA3 expression is associated with breast carcinomas of luminal subtype and low histological grade. PMID: 28428285
    40. The aim of this study was to investigate the clinical significance of three immune cell-related transcription factors, T-bet, GATA-3 and Bcl-6 in bladder cancer in Tunisian patients. PMID: 27237631
    41. Data delineates the regulatory mechanisms of GATA3 in DNA double-strand breaks repair and strongly suggests that it might act as a tumor suppressor by promoting CtIP expression and homologous recombination to stabilize genomes. PMID: 28481869
    42. data provide new insights into the role of SEMA3B in mammary gland and provides a new branch of GATA3 signaling that is pivotal for inhibition of breast cancer progression and metastasis PMID: 28581515
    43. It gene expression is up-regulated by EP300-ZNF384 fusion gene product. PMID: 28378055
    44. Coexpression of GATA3 and CK7 in most clear cell papillary renal cell carcinomas provides evidence of their origin from distal nephron and can be used in differential diagnosis. PMID: 28705707
    45. Our findings suggest that GATA3 stabilizes HIF-1alpha to enhance cancer invasiveness under hypoxia PMID: 28263977
    46. GATA-3 is a sensitive and specific marker for the diagnosis of acute leukemias with T-cell differentiation. PMID: 28551327
    47. In resected lung adenocarcinoma, high GATA3 expression is associated with poorer prognosis for both overall survival and disease free survival. PMID: 28322854
    48. Loss of GATA3 expression was found to be an independent predictor of poor patient outcomes in bladder urothelial carcinoma. PMID: 28428106
    49. Meta-analysis revealed that high expression of GATA3 in breast cancer is associated with improved time to tumor progression. PMID: 28394898
    50. Rs17144046 located near GATA3 was significantly associated with benign prostatic hyperplasia and lower urinary tract symptoms. PMID: 28656603

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  • 相关疾病：
    Hypoparathyroidism, sensorineural deafness, and renal disease (HDR)
  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    T-cells and endothelial cells.
  • 数据库链接：

    HGNC: 4172

    OMIM: 131320

    KEGG: hsa:2625

    STRING: 9606.ENSP00000368632

    UniGene: Hs.524134