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  4. Phospho-FOXO3 (S253) Recombinant Monoclonal Antibody

Phospho-FOXO3 (S253) Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA008836A253phHU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate
      All lanes Phospho-FOXO3 antibody at 0.96μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 97 KDa
      Observed band size: 97 KDa
    • IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA008836A253phHU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品描述：

    The synthesized DNA sequence corresponding to the phospho-FOXO3 (S253) monoclonal antibody was cloned into the plasmid and then transfected into the cell line for expression. The product was purified through the affinity-chromatography method and obtained the phospho-FOXO3 (S253) recombinant monoclonal antibody. This FOXO3-pS253 recombinant antibody is a rabbit IgG and has been tested in scientific applications, including ELISA, WB, and IHC. It can be used to detect the human FOXO3 phosphorylated at Ser253 residue.

    FOXO3 is a transcription factor that controls a variety of physiological processes such as cell cycle arrest, apoptosis, oxidative stress response, and energy consumption. Various post-translational modifications, such as acetylation, ubiquitination, and phosphorylation, regulate FOXO3. AKT phosphorylates FOXO3 at T32 and S253, forming 14-3-3 binding sites, which modulates FOXO3 transcriptional activity and localization.

  • Uniprot No.：
    O43524
  • 基因名：
  • 别名：
    AF6q21 antibody; AF6q21 protein antibody; DKFZp781A0677 antibody; FKHR2 antibody; FKHRL 1 antibody; FKHRL1 antibody; FKHRL1P2 antibody; Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 antibody; Forkhead box O3 antibody; Forkhead box O3A antibody; Forkhead box protein O3 antibody; Forkhead box protein O3A antibody; Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 antibody; Forkhead homolog (rhabdomyosarcoma) like 1 antibody; Forkhead in rhabdomyosarcoma like 1 antibody; Forkhead in rhabdomyosarcoma-like 1 antibody; FOX O3A antibody; FOXO2 antibody; foxo3 antibody; FOXO3_HUMAN antibody; FOXO3A antibody; MGC12739 antibody; MGC31925 antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-FOXO3 (S253)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1E1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy. Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins. Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation. Also acts as a key regulator of regulatory T-cells (Treg) differentiation by activating expression of FOXP3.
  • 基因功能参考文献：
    1. role in autophagy activation and the maintenance of intracellular homeostasis in inflamed odontoblasts PMID: 29551204
    2. Low FOXO3A expression is associated with colorectal cancer. PMID: 30066886
    3. FoxO3a was overexpressed in 64.71% cases of hepatocellular carcinoma (HCC). FoxO3a overexpression was associated with aggressive phenotypes of HCC, such as histologic grade, stage, and small vessel invasion. FoxO3a overexpression was also correlated with poor disease-free survival. Downregulation of FoxO3a in a HepG2 cell line inhibited cell proliferation and migration. PMID: 29365018
    4. stable knockdown of FOXO3, NCOA3, and TCF7L2 restored growth in low glucose but reduced MEK/MAPK phosphorylation, reduced anchorage-independent growth, and modulated expressions of GLUT1 and Ras pathway related proteins. PMID: 29301589
    5. the inhibition of miR-9 could induce apoptosis in cervical cancer by targeting FOXO3. PMID: 29602130
    6. Study in three European populations present experimental evidence for a functional link between common intronic variants in FOXO3 and human longevity. PMID: 29234056
    7. circRNA-FOXO3 expression was decreased in NSCLC cells and tissue samples. It can inhibit the development of NSCLC cells as a ceRNA through sponging miR-155 and releasing FOXO3 level. PMID: 29620202
    8. The protein expression levels of several autophagy makers, such as LC3I, LC3II and Beclin-1, were higher in FOXO3 plasmid-transfected AGS cells cultured in an acidic microenvironment than in control cells, while P62 protein expression levels were clearly decreased in FOXO3 plasmid-transfected cells compared with control cells. PMID: 30138933
    9. Study suggests that miR-487a-3p might repress CTLA4 and FOXO3 by binding to their 3'UTRs and contribute to the development of T1D. PMID: 29859273
    10. Findings determined that the crucial regions corresponding to the SP1 binding sites located between 2,000 and 1,037 bp were essential for FoxO3a transcriptional activity. Furthermore, FoxO3a transcription was upregulated in response to hypoxic and oxidative stress in colorectal tumor cells (CRC), indicating that the interaction between SP1 and FoxO3a may have important implications in CRC progression. PMID: 29565456
    11. FOXO3a expression correlated with adverse clinicopathological features, such as lymph node metastasis, perineural invasion and higher Ki-67 proliferation index in triple-negative breast cancers. PMID: 29588373
    12. human FOXO3B locus encodes a bona fide human gene; unlike FOXO3A, FOXO3B is cytosolically localized in both the presence and absence of active Akt PMID: 29925039
    13. FoxO3a overexpression increased the transcription and protein expression of Bcl2like protein 11 and cyclindependent kinase inhibitor 1B, and inhibited cyclin D1 transcription and expression. PMID: 29257235
    14. miR-132 negatively regulates palmitate induced NLRP3 inflammasome activation through FOXO3 down-regulation in THP-1 cells. PMID: 29258239
    15. these data ascertain the existence of an H2O2-sensitive PRDX1-FOXO3 signaling axis that fine tunes FOXO3 activity toward the transcription of gene targets in response to oxidative stress. PMID: 28398822
    16. results suggested that SIRT1 deficiency in Bladder cancer cells could suppress cell viability by activating antioxidant response and inducing cell cycle arrest possibly via FOXO3a-related pathways. PMID: 29147649
    17. these results suggest that miR-30b plays important roles in kynurenine-induced increase of FOXO3 expression PMID: 28905195
    18. s found that miR-629 negatively regulated FOXO3 protein expression and decreased the activity of a luciferase reporter construct containing the FOXO3 3'-untranslated region. These results show that miR-629 regulates FOXO3 at the posttranscriptional level, resulting in enhanced cell proliferation and invasion of pancreatic carcinoma. PMID: 29072689
    19. auranofin could regulate the Her2/Akt/FOXO3 signaling pathway in SKOV3 cells and be used as a potential antitumor agent considering the expression of MUC4 in ovarian cancer patients. PMID: 28765909
    20. FNDC5 gene interactions with candidate genes FOXOA3 and APOE PMID: 29143599
    21. Results indicate a mechanism for beta-arrestin1 in the regulation of the prostate cancer procession through inhibiting FOXO3a. PMID: 29676828
    22. The findings of this study indicated that FoxO3a knockdown conferred neuroprotective effects after TBI through inhibiting the activation of neuronal autophagy. PMID: 28889023
    23. Low FOXO3A expression is associated with cancer. PMID: 29533771
    24. Data show that forkhead box O3 protein (FOXO3) silencing could inhibit mitophagy and mitochondrial dysfunction induced by manganese chloride (MnCl2). PMID: 28661534
    25. Report an inverse relationship of age with human serum FOXO3A and SIRT3 levels. PMID: 28526626
    26. FOXO3 longevity interactome on chromosome 6 has been described. PMID: 28722347
    27. miR-223-3p regulated cell chemo-sensitivity by targeting FOXO3 in prostatic cancer (PCa) both in vitro and in vivo, providing new potential therapeutic strategy for PCa treatment. PMID: 29518547
    28. H cordata promotes the activation of HIF-1A-FOXO3 and MEF2A pathways. PMID: 27698266
    29. A lower FOXO3 mRNA expression in granulosa cells leads to poor oocyte development in patients with unexplained infertility undergoing controlled ovarian stimulation for in vitro fertilization-embryo transfer. PMID: 28621049
    30. Negative expression of FoxO3/FoxO4 and lymph node metastasis were the risk factors for the poor prognosis of bladder cancer. PMID: 28554751
    31. miR-155-5p promotes fibroblast cell proliferation and inhibits FOXO signaling pathway by negative modulation of both FOXO3 and CDKN1B in vulvar lichen sclerosis PMID: 29339071
    32. Cytoplasmic retention of FOXO3a may represent a potential biomarker for response to combined treatment with inhibitors of PI3K and autophagy in PIK3CA-mutant cervical cancer cells. PMID: 28036259
    33. Results show that FOXO3-phosphorylation at threonine-32 (T32) and nuclear localization in neuroblastoma biopsies significantly correlated with stage IV disease. Data suggest that, depending on the mode and intensity of activation, cellular FOXO3 acts as a homeostasis regulator promoting tumor growth at hypoxic conditions and tumor angiogenesis in high-stage neuroblastoma. PMID: 27769056
    34. FoxO3a might be a key regulator in cetuximab resistance through up-regulating c-Myc in colorectal cancer targeted therapy. PMID: 27825133
    35. Atorvastatin strengthens Skp2 binding to FOXO1 or ICAM1, leading to ubiquitination and degradation. Skp2-dependent ubiquitination of major pathogenic molecules is the key mechanism for statin's protective effect on endothelial function in diabetes. PMID: 28802579
    36. Transcriptional factor PAX3 (PAX3) exerted its tumor suppressor function by inhibiting the activity of major signaling pathways and enhancing expression and activity of transcription factor forkhead box O3 protein (FOXO3a). PMID: 27458157
    37. The result suggests that FOXO3 rs12212067 polymorphism does not play an important role in susceptibility to T. cruzi infection and/or chronic Chagas cardiomyopathy. PMID: 27125259
    38. Overexpression of circ-Foxo3 decreased the interaction between Foxo3 and MDM2, and repressed the function of MDM2 in modulating poly-ubiquitination of Foxo3. PMID: 27886165
    39. silencing FOXO3 diminishes bepridil- and trifluoperazine-induced apoptosis in triple-negative breast cancer cells PMID: 27283899
    40. The s found that transient TUBB3 activation, through ABCB1, in response to the stimulation of FOXO3a expression, significantly contributes to the cross-resistance of the paclitaxel-resistant cell population and consequently limits the efficacy of both agents where cancer cells have developed multiple resistance. PMID: 27284014
    41. findings provide further evidence for the involvement of FoxO3 during terminal erythropoiesis and confirm the modulation of the PI3K/AKT pathway as a potential therapeutic strategy for beta-thalassemia PMID: 29099866
    42. The data reveal a previously unexplored function of FOXO3a in gastric cancer invasion by regulating proteins involved in extracellular matrix degradation and Epithelial-Mesenchymal Transition. FOXO3a may be of prognostic value and a potential therapeutic target in blocking tumor metastasis. PMID: 27127880
    43. pro-apoptotic role of miR-34a in PA-induced cholangiocyte lipoapoptosis in culture and in the liver PMID: 28250026
    44. Ergosterol peroxide stimulated Foxo3 activity by inhibiting pAKT and c-Myc and activating pro-apoptotic protein Puma and Bax to induce HepG2 cancer cell death. PMID: 27058618
    45. These results show that significantly increased levels of FOXO3, IRF4, and xIAP mRNA in Chinese HIV-1-infected patients. PMID: 27841661
    46. Knockdown of MIEF2 reduces DOX-induced mitochondrial fission and apoptosis in cardiomyocytes and in vivo. Also, knockdown of MIEF2 protects heart from DOX-induced cardiotoxicity. Our study identifies a novel pathway composed of Foxo3a and MIEF2 that mediates DOX cardiotoxicity. PMID: 28137654
    47. data show that the GSK3B-FOXO3 pathway is activated after partial hepatectomy, and this may be one of the mechanisms that lead to upregulation of hepatic IGF1R after partial hepatectomy. PMID: 28952285
    48. Chromosome 6q deletion correlates with poor prognosis and low relative expression of FOXO3 in chronic lymphocytic leukemia patients. PMID: 28699185
    49. Diabetic Glc also promoted beta-catenin nuclear localization and the formation of a complex with FOXO3a that localized to the promoters of Sod2, p21(cip1), and potentially p27(kip1) PMID: 27411103
    50. first report of the association between rs13217795 and allergic rhinitis, and the first independent verification of the association between rs13217795 and asthma PMID: 29141605

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  • 相关疾病：
    A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with KMT2A/MLL1.
  • 亚细胞定位：
    Cytoplasm, cytosol. Nucleus. Mitochondrion matrix. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 3821

    OMIM: 602681

    KEGG: hsa:2309

    STRING: 9606.ENSP00000339527

    UniGene: Hs.220950