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  4. Phospho-ATF2 (T71) Recombinant Monoclonal Antibody

Phospho-ATF2 (T71) Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA002270A71phHU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in 293 whole cell lysate,A549 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or EGF)
      All lanes Phospho-ATF2 antibody at 1.015μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 70 KDa
      Observed band size: 70 KDa
    • Immunofluorescence staining of A549 cells(treated with 100mM EGF for 20min) with CSB-RA002270A71phHU at 1:63,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    Phospho-ATF2 (T71) recombinant monoclonal antibody was prepared by cloning the coding sequence for the phospho-ERN1 (S724) monoclonal antibody (produced by immunizing animals with the synthetic phosphopeptide of ERN1) into the plasmids and transfecting the clones into cell lines. It is a rabbit IgG purified through the affinity-chromatography method. This phospho-ATF2 (T71) antibody detects endogenous levels of human ATF2only when phosphorylated at T71. It can be applied in ELISA, WB, and IF analyses.

    ATF2 is found in almost all cell types and regulates the transcription of genes involved in a wide range of biological processes, including cell growth, development, and stress. ATF2 participates in the transmission of extracellular signals to the nucleus, promoting transcriptional responses to stimuli. Many stimuli, such as growth hormones, UV radiation, and cytokines, can activate ATF2. Stress-activated protein kinases (SAPKs) (e.g., p38) phosphorylate ATF2 at Thr69 and Thr71, which activates transcriptional activation of ATF2. ATF2 can interact with other AP1 proteins and translocate to the nucleus after being phosphorylated at T69/T71, allowing it to influence gene expression.

  • Uniprot No.：
    P15336
  • 基因名：
  • 别名：
    Activating transcription factor 2 antibody; Activating transcription factor 2 splice variant ATF2 var2 antibody; ATF 2 antibody; Atf-2 antibody; Atf2 antibody; ATF2 protein antibody; ATF2_HUMAN antibody; cAMP Response Element Binding Protein 2 antibody; cAMP response element binding protein CRE BP1 antibody; cAMP response element-binding protein CRE-BP1 antibody; cAMP responsive element binding protein 2; formerly antibody; cAMP-dependent transcription factor ATF-2 antibody; cAMP-responsive element-binding protein 2 antibody; CRE BP1 antibody; CRE-BP antibody; CREB 2 antibody; CREB-2 antibody; CREB2 antibody; CREBP1 antibody; Cyclic AMP dependent transcription factor ATF 2 antibody; Cyclic AMP-dependent transcription factor ATF-2 antibody; Cyclic AMP-responsive element-binding protein 2 antibody; D130078H02Rik antibody; D18875 antibody; HB 16 antibody; HB16 antibody; Histone acetyltransferase ATF2 antibody; MGC105211 antibody; MGC105222 antibody; MGC111558 antibody; MGC142504 antibody; mXBP antibody; MXBP protein antibody; Tg(Gzma-Klra1)7Wum antibody; TREB 7 antibody; TREB7 antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human Phospho-ATF2 (T71)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4F2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro. In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
  • 基因功能参考文献：
    1. Our study found that miR-451 regulates the drug resistance of renal cell carcinoma by targeting ATF-2 PMID: 28429654
    2. deregulation of the miR-144-5p/ATF2 axis plays an important role in non-small-cell lung cancer cell radiosensitivity. PMID: 29850528
    3. p38alpha and ATF2 expression play a crucial role in the malignant phenotypes of ovarian tumor cells and are a markers of poor prognosis in patients with ovarian serous adenocarcinomas. PMID: 28916425
    4. activation of JNK was found to be dependent on muscarinic acid receptor induced Ca(2+)/CAMKII as well as ROS. JNK dependent phosphorylation of ATF2/c-Jun transcription factors resulted in TGF-beta transcription and its signaling. PMID: 27708346
    5. ATF2 regulated by miR-204 might also play an important role in the regulation of malignant behavior of glioblastoma. PMID: 27588402
    6. We further demonstrated the suppressive function of lncRNA#32 in hepatitis B virus and hepatitis C virus infection. lncRNA#32 bound to activating transcription factor 2 (ATF2) and regulated ISG expression. Our results reveal a role for lncRNA#32 in host antiviral responses. PMID: 27582466
    7. Results show that ATF2 is highly expressed in renal cell carcinoma (RCC) tissues and promotes RCC cell proliferation, migration and invasion. The study suggests that ATF2 exerts an oncogenic role in RCC. PMID: 27377902
    8. These findings point to an oncogenic function for ATF2 in melanoma development that appears to be independent of its transcriptional activity. PMID: 27210757
    9. this study demonstrates that CPEB2 alternative splicing is a major regulator of key cellular pathways linked to anoikis resistance and metastasis. PMID: 28904175
    10. Noxin facilitated the expression of Cyclin D1 and Cyclin E1 through activating P38-activating transcription factor 2 signaling pathway, thus enhanced cell growth of breast cancer PMID: 28618963
    11. these observations suggest that CD99 is involved in the regulation of CD1a transcription and expression by increasing ATF-2. PMID: 27094031
    12. This review provides an overview of the currently known upstream regulators and downstream targets of ATF2. [review] PMID: 28212892
    13. TNF induces the binding of ATF2 to the TNF-responsive element. PMID: 27821620
    14. miR-204 may act as a tumor suppressor by directly targeting ATF2 in non-small cell lung cancer PMID: 26935060
    15. the variant alleles of TSG101 rs2292179 and ATF2 rs3845744 were associated with a reduced risk of breast cancer, particularly for subjects with BMI <24 (kg/m(2)) and postmenopausal women, respectively PMID: 26729199
    16. Results reveal that mitochondrial ATF2 is associated with the induction of apoptosis and BRAF inhibitor resistance through Bim activation. PMID: 26462148
    17. Neisseria meningitidis caused a high level of E-selectin expression elicited by the activity of phosphorylated ATF2 transcription factor on the E-selectin promoter. PMID: 26153406
    18. increased expression of the gene encoding PKCepsilon and abundance of phosphorylated, transcriptionally active ATF2 were observed in advanced-stage melanomas and correlated with decreased FUK expression PMID: 26645581
    19. CARMA1- and MyD88-dependent activation of Jun/ATF-type AP-1 complexes is a hallmark of ABC diffuse large B-cell lymphomas. PMID: 26747248
    20. More terminally differentiated human odontoblasts was ATF-2 positive, as compared to pulpal fibroblasts at various stages of differentiation: ATF-2 is more associated with cell survival rather than cell proliferation. PMID: 25417007
    21. Study identified a potential target of miR-451, ATF2, and revealed a novel role of miR-451 in the inhibition of the migratory ability of hepatoma cell lines. PMID: 24968707
    22. ATF-2 knockdown blocked VEGF-A-stimulated VCAM-1 expression and endothelial-leukocyte interactions. ATF-2 was also required for other endothelial cell outputs, such as cell migration and tubulogenesis. PMID: 24966171
    23. Study demonstrates the role of miR-622 in suppressing glioma invasion and migration mediated by ATF2, and shows that miR-622 expression inversely correlates with ATF2 in glioma patients PMID: 25258251
    24. suppression of tumorigenesis by JNK requires ATF2. PMID: 25456131
    25. study revealed that, autocrine soluble factors regulate dual but differential role of ATF-2 as a transcription factor or DNA repair protein, which collectively culminate in radioresistance of A549 cells. PMID: 25041846
    26. While expression of ATF-2 is not associated with outcome. PMID: 25141981
    27. The expression of ATF2 in chondrocytes is involved in apoptosis in Kashin-Beck disease. PMID: 23866832
    28. in human HCC tissues, SPTBN1 expression correlated negatively with expression levels of STAT3, ATF3, and CREB2; SMAD3 expression correlated negatively with STAT3 expression PMID: 25096061
    29. zymosan-induced il23a mRNA expression is best explained through coordinated kappaB- and ATF2-dependent transcription; and (iii) il23a expression relies on complementary phosphorylation of ATF2 on Thr-69 and Thr-71 dependent on PKC and MAPK activities. PMID: 24982422
    30. Data show that salvianolic acid B protects endothelial progenitor cells against oxidative stress by modulating Akt/mTOR/4EBP1, p38 MAPK/ATF2, and ERK1/2 signaling pathways. PMID: 24780446
    31. There is synergism between developmental stage-specific recruitments of the ATF2 protein complex and expression of gamma-globin during erythropoiesis. PMID: 24223142
    32. An association between ATF2 polymorphisms and heavy alcohol consumption is only weakly supported. PMID: 24338393
    33. ATF2 knockdown revealed ATF2-triggered p21(WAF1) protein expression, suggesting p21(WAF1) transactivation through ATF2. PMID: 23800081
    34. results therefore suggest that c-MYC induces stress-mediated activation of ATF2 and ATF7 and that these transcription factors regulate apoptosis in response to oncogenic transformation of B cells PMID: 23416976
    35. we establish that ATF2 family members physically and functionally interact with TCF1/LEF1 factors to promote target gene expression and hematopoietic tumor cell growth PMID: 23966864
    36. Cytoplasmic ATF2 expression was less frequently seen than nuclear expression in malignant mesenchymal tumors. Benign mesenchymal tumors mostly showed much lower nuclear and cytoplasmic ATF2 expression. PMID: 24289970
    37. Data indicate that small molecules that block the oncogenic addiction to PKCepsilon signaling by promoting ATF2 nuclear export, resulting in mitochondrial membrane leakage and melanoma cell death. PMID: 23589174
    38. Increasing of ATF2 expression is mediated via oxidative stress induced by arsenic in SV-HUC-1 cells, and MAPK pathways are involved. PMID: 23591579
    39. these studies show that the IL-1beta-induced increase in intestinal tight junction permeability was regulated by p38 kinase activation of ATF-2 and by ATF-2 regulation of MLCK gene activity PMID: 23656735
    40. Phosphorylation of ATF2 by PKCepsilon is the master switch that controls its subcellular localization and function. PMID: 22685333
    41. ATF2-Jun heterodimers bind IFNb in both orientations alone and in association with IRF3 and HMGI PMID: 22843696
    42. we report the kinetic mechanism for JNK1beta1 with transcription factors ATF2 and c-Jun along with interaction kinetics for these substrates. PMID: 22351776
    43. ATF2 subcellular localization is probably modulated by multiple mechanisms PMID: 22275354
    44. Data concluded that IR-induced up-regulation of ATF2 was coordinately enhanced by suppression of miR-26b in lung cancer cells, which may enhance the effect of IR in the MAPK signaling pathway. PMID: 21901137
    45. The ability of ATF2 to reach the mitochondria is determined by PKCepsilon, which directs ATF2 nuclear localization. Genotoxic stress attenuates PKCepsilon effect on ATF2; enables ATF2 nuclear export and localization at the mitochondria. PMID: 22304920
    46. Data show that ATF7-4 is an important cytoplasmic negative regulator of ATF7 and ATF2 transcription factors. PMID: 21858082
    47. Our data suggest regulatory roles for ATF2 in TNF-related mechanisms of Head and Neck Squamous Cell Carcinoma. Its perturbation and nuclear activation are associated with significant effects on survival and cytokine production. PMID: 21990224
    48. Data suggest that competition between GSTpi and active JNK for the substrate ATF2 may be responsible for the inhibition of JNK catalysis by GSTpi. PMID: 21384452
    49. ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and beta2, and activates insulin gene transcription. PMID: 21278380
    50. MITF is downregulated by ATF2 in the skin of Atf2-/- mice, in primary human melanocytes, and in melanoma cell lines. PMID: 21203491

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Mitochondrion outer membrane. Note=Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co-localizes with the MRN complex in the IR-induced foci (IRIF).
  • 蛋白家族：
    BZIP family, ATF subfamily
  • 组织特异性：
    Ubiquitously expressed, with more abundant expression in the brain.
  • 数据库链接：

    HGNC: 784

    OMIM: 123811

    KEGG: hsa:1386

    STRING: 9606.ENSP00000264110

    UniGene: Hs.592510