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PLAU Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA963043A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA963043A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    PLAU, also called Urokinase-type plasminogen activator (uPA), binds to uPAR inducing migration, adhesion, and proliferation through multiple interactions with G protein-coupled receptor FPRL1, integrins, or the epidermal growth factor (EGF) receptor (EGFR). The serine protease uPA and its high-affinity cell surface receptor uPAR play an important role in various physiological as well as pathological extracellular degradation processes, where cell migration is required, such as inflammatory responses and tumor invasion. Noelia Lino et al. showed that uPA has a key role during the central nervous system (CNS) development. In association with its receptor, it orchestrates both proteolytic and nonproteolytic events that govern the proper formation of neural networks.

    The main steps in the production of this PLAU recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The PLAU antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, IHC.

  • Uniprot No.：
    P00749
  • 基因名：
  • 别名：
    Urokinase-type plasminogen activator (U-plasminogen activator) (uPA) (EC 3.4.21.73) [Cleaved into: Urokinase-type plasminogen activator long chain A, Urokinase-type plasminogen activator short chain A, Urokinase-type plasminogen activator chain B], PLAU
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Urokinase
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    10E5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin.
  • 基因功能参考文献：
    1. The combination of decoy receptor 3, soluble urokinase type plasminogen activator receptor, and procalcitonin improved the sensitivity and specificity of diagnosis of sepsis, suggesting that use of the combination of three indexes enhanced the efficiency of sepsis diagnosis. PMID: 29760745
    2. High Expressions of PLAU is associated with lung adenocarcinoma. PMID: 28454121
    3. Increased expression of uPA in epidermal cells in psoriasis and in tumor cells in basal cell carcinomas suggests an important role of the uPA system for aggressively proliferating and invading cells of epidermal origin. PMID: 28429105
    4. study provides strong support in the role of L. reuteri in suppression of GC cell invasion by downregulation of pathways which is involved in extracellular matrix degradation such as uPA and uPAR PMID: 28643742
    5. Gelsolin enhances the invasive capacity of colon cancer cells via elevating intracellular superoxide (O2.-) levels by interacting with Cu/ZnSOD, and gelsolin gene expression positively correlates with urokinase plasminogen activator (uPA), an important matrix-degrading protease invovled in cancer invasion. PMID: 27391159
    6. Study provides evidence that the stimulation of u-PA/u-PAR system contributes to the activated phenotype and function of cancer-associated fibroblasts during multiple myeloma. PMID: 28340565
    7. OB-Rb, RhoA/ROCK, PI3K/AKT, JAK/STAT pathways and NF-kB activation are involved in leptin-induced upA expression. PMID: 28104444
    8. Results provide evidence that uPA and IGF1R directly interact with uPAR enhancing malignant potential of triple-negative breast cancer. PMID: 27502396
    9. suggest that the low endogenous levels of uPA in blood are actively regulated, and that the regulatory mechanisms are disrupted in QPD in a megakaryocyte-specific manner PMID: 28301587
    10. an intricate link between caveolin-1 and Src kinase-mediated cell signaling and alveolar epithelial cell apoptosis due to loss of SP-C expression through p53 and uPA system-mediated cross-talk, is reported. PMID: 28385810
    11. results show that the uPA/uPAR/LRP1 system is a potential target for the development of therapeutic strategies to promote axonal recovery following a CNS injury PMID: 27986809
    12. The present research concluded that aspirin suppressed prostate cancer cell invasion by reducing MMP-9 activity and uPA expression through decreasing of IKK-beta-mediated NF-kappaB activation, indicating that the ability of aspirin to inhibit cell invasion might be useful in the chemoprevention of metastatic prostate cancer. PMID: 28278500
    13. These studies identify uPA-dependent de-repression of vegfr1 and vegfr2 gene transcription through binding to HHEX/PRH as a novel mechanism by which uPA mediates the pro-angiogenic effects of VEGF and identifies a potential new target for control of pathologic angiogenesis. PMID: 27151212
    14. The up-regulation of uPA mRNAs was correlated with high-risk clinicopathological features, including extrathyroid invasion, loss of cellular polarity/cohesiveness, and the BRAF(V600E) mutation. PMID: 25085839
    15. Transplantation of uPA gene modified mesenchymal stem cells suppressed liver fibrosis and ameliorated liver function and may be a new approach to treating liver fibrosis PMID: 26877613
    16. ApaL1 and Taq1 single nucleotide polymorphisms of the urokinase and VDR genes are associated with recurrent urolithiasis in a Caucasian population. PMID: 26275878
    17. Resveratrol inhibited hypoxia-induced HIF-1alpha protein expression. Resveratrol also suppressed hypoxiainduced expression of metastatic-related factors, uPA and MMP2. PMID: 26707376
    18. Significance of the urokinase-type plasminogen activator and its receptor in the progression of focal segmental glomerulosclerosis in clinical and mouse models. PMID: 26846181
    19. Data suggest that enhanced levels of uPA in breast cancer modulate the mitogenic effects of EGF which helps to better understand breast cancer pathogenesis. PMID: 25641046
    20. Results found high levels of uPA and uPAR exclusively in metastatic osteosarcoma (OS)cells and suggest that malignant conversion of OS cells to uPA/uPAR axis in an autocrine and paracrine fashion. PMID: 26317203
    21. The morphologically normal tissue adjacent to the tumor shows the substantial expression of MMP-2 and MMP-9 and in some cases the enhanced activity of uPA and ACE, which makes an additional contribution to the increased invasive potential of tumor PMID: 26978018
    22. it was found that the recombinant fusion protein uPA17-34-KPI(kunitz-type protease inhibitor ) inhibited the invasion and metastasis of ovarian tumors PMID: 26166362
    23. Crystal structure of uPA bound with cyclic peptidic inhibitors. PMID: 25744057
    24. u-PA is a dysregulated gene involved in the tumorigenesis, bio-pathological features and outcomes of EEC. PMID: 26033187
    25. High cytoplasmic expression of uPA is associated with cells of rectal cancer and metastases of perienteric lymph nodes. PMID: 26504024
    26. DIM can influence the cell migratory and invasive properties of human colorectal cancer cells and may decrease the invasive capacity of colorectal cancer through downregulation of uPA and MMP9 mediated by suppression of the transcription factor FOXM1 PMID: 25962429
    27. Porphyromonas gingivalis-derived RgpA-Kgp complex activates the macrophage uPA. PMID: 25979345
    28. SNP P141L associated with collateral circulation in coronary artery disease PMID: 24952395
    29. Taking uPA(1-43) amino acids specifically binding to uPAR as targeted part of fusion protein, and making use of antitumor activity of melittin, the recombinant fusion protein it was able to inhibit growth of ovarian tumors . PMID: 25394558
    30. uPA/uPAR stimulates triglyceride synthesis in Huh7 hepatoma cells via p38-dependent upregulation of DGAT2 PMID: 25244504
    31. Studied urokinase-type plasminogen activator regulation in cigarette smoke extract (CSE)-induced EMT in human small airway epithelial cell lines (HSAEpiCs)and in the small airway epithelium of COPD patients. PMID: 25706093
    32. Silencing of ETV4 suppresses invasion of prostate cancer cells by inhibiting the expression of uPA/uPAR as well as (MMP)-2 and MMP-9 PMID: 25544710
    33. miR-193b inhibits the expression of stathmin 1 (STMN1) and urokinase-type plasminogen activator (uPA) in Panc-1 cells. PMID: 25215905
    34. Activation of uPA and MMP-3 in perifocal zone of the tumor can serve as an indexof its invasive activity. PMID: 25993872
    35. These data support the hypothesis that uPA upregulation stimulated by macrophages may play an active role in the onset of osteoarthritis and in the shear-stress protection against this induction. PMID: 23597113
    36. High preprocedural plasma uPA and serum CRP levels are indicative of angina recurrence after successful PTCA, and are valuable for the prognosis of restenosis. PMID: 24057344
    37. Data indicate that urokinase-type plasminogen activator (uPA) activates epithelial sodium channel, gamma subunit (ENaC gamma) through catalytic activity-dependent proteolytic modification. PMID: 25555911
    38. IL-17 may act as chemotropic factor for peripheral blood-Mesenchymal stem cellss by incrementing cell motility and uPA expression during inflammation development. PMID: 25433194
    39. Suggest a possible protective effect of podocyte uPA/uPAR expression against interstitial fibrosis in IgA nephritis. PMID: 24648800
    40. High u-PA expression is associated with metastasis in breast cancer. PMID: 24610082
    41. In human coronary vascular smooth muscle, uPA induces uPAR-independent, domain-dependent smooth muscle cell proliferation through transactivation of EGFR by a plasmin-mediated, ADAM-induced, and HB-EGF-dependent process. PMID: 25082749
    42. IL-33 is involved in u-PA-driven angiogenesis, a process that has been shown before to be linked to inflammation in various pathologies. PMID: 24702774
    43. IL-1beta-induced urokinase plasminogen activator expression via activation of the ERK1/2 and NF-kappaB pathways, which results in invasion of gastric cancer cells. PMID: 24626561
    44. Studied the relationship between uPA gene polymorphisms and clinical manifestations of idiopathic membranous nephropathy. PMID: 24822208
    45. These effects were mediated by the efficient conversion of pro-uPA to active uPA and high phosphorylation levels of ERK detected in the PANC-1 cells expressing exogenous polyserase-1. PMID: 24756697
    46. We found no association between allele frequency and risk of multiples sclerosis for any single nucleotide polymorphism investigated for PLAU PMID: 23897640
    47. results of this study suggest that uPA decreases the removal of HDL-cholesteryl ester in the liver via suppression of the hepatic scavenger receptor class B type I (SR-BI)expression PMID: 24529115
    48. Oncogenic K-Ras and loss of Smad4 mediate invasion by activating an EGFR/NF-kappaB Axis that induces expression of MMP9 and uPA in human pancreas progenitor cells. PMID: 24340014
    49. The findings provide evidence that the rs2227564 C/T SNP in the u-PA gene is associated with the development of oral tongue squamous cell carcinoma. PMID: 23621237
    50. Decreasing uPA levels sensitized cancer cells to TRAIL, leading to markedly increased apoptosis. PMID: 24481457

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  • 相关疾病：
    Quebec platelet disorder (QPD)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Expressed in the prostate gland and prostate cancers.
  • 数据库链接：

    HGNC: 9052

    OMIM: 191840

    KEGG: hsa:5328

    STRING: 9606.ENSP00000361850

    UniGene: Hs.77274