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  4. MYBBP1A Recombinant Monoclonal Antibody

MYBBP1A Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA978157A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA978157A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HepG2 Cells with CSB-RA978157A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    MYBBP1A, also known as p160, acts as a co-repressor of multiple transcription factors involved in many physiological processes. In all these processes, MYBBP1A acts as a tumor suppressor, regulating the evolution and malignity of cells. MYBBP1A is also a repressor of PGC1α and can be a key regulator of metabolic processes. MYBBP1A interacts with the negative regulatory domain (NRD) of PGC1α and reduces its ability to stimulate mitochondrial respiration and electron transport system-related gene expression. MYBBP1A also binds to factors involved in epigenetic regulation. MYBBP1A has been linked to cell cycle control, especially the G2/M phase, and mitosis.

    The recombinant MYBBP1A antibody was generated in vitro through inserting cloned MYBBP1A genes into expression vectors. The expression vector was then inserted into a mammalian cell to express this MYBBP1A antibody. It has been validated in ELISA, IHC, IF. Every step in the production was controlled strictly.  You have no worries about the quality.

  • Uniprot No.：
    Q9BQG0
  • 基因名：
    MYBBP1A
  • 别名：
    Myb-binding protein 1A, MYBBP1A, P160
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human MYBBP1A
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3A1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2. Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter. Has a role in rRNA biogenesis together with PWP1.
  • 基因功能参考文献：
    1. The results indicated that both the heterozygous genotype GC and homozygous genotype CC in rs3809849 in MYBBP1A had significant effects on the risk of pulmonary tuberculosis, and heterozygous genotype CT in rs9061 in SP110 also had similar effects. PMID: 25612917
    2. We propose that the nucleolus functions as a stress sensor to modulate p53 protein levels and its acetylation status, determining cell fate between cell cycle arrest and apoptosis by regulating MYBBP1A translocation. PMID: 26044764
    3. MYBBP1A(low)AKT(Ser473)(high) staining pattern serves not only as a marker for the pre-senescent stage but also as an indicator of OPSCC patients at high risk for treatment failure. PMID: 25543088
    4. TPPII, MYBBP1A and CDK2 form a protein-protein interaction network. PMID: 25303791
    5. MYBBP1A functions to enhance p53 tetramerization that is necessary for p53 activation. PMID: 24375404
    6. Mybbp1a is a novel negative regulator of Sirt7. PMID: 24134843
    7. Regulators, including BCL11A, MYB, and KLF1, hold great promise to develop targeted and more effective approaches for HbF induction. PMID: 23209159
    8. MYBBP1A-p53 binding property can account for efficient p53-activation by MYBBP1A under nucleolar stress. Our results support the idea that MYBBP1A plays catalytic roles in p53 acetylation and activation PMID: 23583237
    9. Suggest that MYBBP1A is required for p53 activation during anoikis; therefore, it is involved in suppressing colony formation and the tumorigenesis of breast cancer cells. PMID: 23388179
    10. Data show that down-regulation of MYBBP1A decreases the growth rate of wild type mouse embryonic stem cells, embryo fibroblasts (MEFs) and of human HeLa cells, where it also promotes apoptosis. PMID: 23056166
    11. Study suggests that a combination of SP110 and MYBBP1A gene polymorphisms may serve as a novel marker for identifying the risk of developing TB in the Chinese Han population. PMID: 23129390
    12. Results from our present work reveal a previously unrecognized co-repressor role of Mybbp1a in rRNA expression. PMID: 22686419
    13. Mybbp1a may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes. PMID: 22645127
    14. We provide experimental evidence that MYBBP1A is an important molecular switch in the regulation of tumor cell proliferation versus migration in head and neck squamous cell carcinoma cells. PMID: 22339894
    15. When rRNA transcription was suppressed by nucleolar stress, MYBBP1A translocated to the nucleoplasm and facilitated p53-p300 interaction to enhance p53 acetylation. PMID: 21297583
    16. identified MYBBP1A as a novel Aurora B substrate and serine 1303 as the major phosphorylation site PMID: 20177074
    17. Myb-binding protein 1a was identified in anti-SMN pulldowns as interacting with SMN proteins. PMID: 19928837
    18. These results indicate that MYBBP1a is a novel NF-kappaB co-repressor of transcription that competes with p300 and may function to regulate cell type specific genes. PMID: 17196614
    19. Expression of Ets-2, SRC-1 and c-Myc individually are all associated with reduced disease-free survival in breast cancer PMID: 18059336
    20. SRC-1 is a strong independent predictor of reduced disease free survival, whereas the interactions of the p160 proteins with estrogen receptor alpha can predict the response of patients to endocrine treatment. PMID: 19276281

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, nucleolus.
  • 数据库链接：

    HGNC: 7546

    OMIM: 604885

    KEGG: hsa:10514

    STRING: 9606.ENSP00000370968

    UniGene: Hs.22824