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  4. MAOB Recombinant Monoclonal Antibody

MAOB Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA999377A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: L02 whole cell lysate, HepG2 whole cell lysate
      All lanes: Monoamine Oxidase B Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 59, 47 kDa
      Observed band size: 59 kDa
    • IHC image of CSB-RA999377A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA999377A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    MAOB is a FAD-dependent mitochondrial enzyme responsible for the metabolism of biogenic amines as well as amine xenobiotics. MAOB metabolizes dopamine and is implicated in the generation of free radicals by dopamine. Inhibition of MAOB reduces dopamine turnover and oxidative stress. MAOB suppression also increases the half-life of dopamine in the synaptic cleft and enhances receptor stimulation and reuptake of dopamine into the presynaptic bulb.

    Compared with the polyclonal and monoclonal antibodies of MAOB, this MAOB recombinant antibody has the features of increased reproducibility and control, animal-free technology, high degree of monovalency, high batch-to-batch consistency, easier isotype conversion, etc. And it has been validated in ELISA, WB, IHC.

  • Uniprot No.：
    P27338
  • 基因名：
  • 别名：
    Amine oxidase [flavin-containing] B (EC 1.4.3.4) (Monoamine oxidase type B) (MAO-B), MAOB
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Monoamine Oxidase B
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9B6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
  • 基因功能参考文献：
    1. Our observations provide new information on the genotype-phenotype relations of MAOA/B and EFHC2 genes involved in the contiguous deletions of Norrie disease.Based on the case of our observation, contiguous deletion with only one of the MAO genes (MAOB) may not cause psychomotor disability, and deletion of EFHC2may not contribute to epilepsy. PMID: 29321361
    2. This study shows that MAO-B levels are increased not only in astrocytes but also in pyramidal neurons in Alzheimer disease brain and also suggests that MAO-B regulates Abeta production in neurons via gamma-secretase PMID: 28764767
    3. The findings may suggest that MAO-B is an important sensor in iron-stressed neuronal cells. PMID: 28685208
    4. brain protein levels of MAOB are normal or elevated in the three parkinsonian conditions-with MAOB increase generally associated with elevations in levels of astrocyte markers. Brain MAOA concentrations were, somewhat surprisingly, not decreased in Parkinson's disease, Progressive Supranuclear palsy or multiple system atrophy with the exception of the atrophic putamen in MSA, despite loss of dopamine neurons. PMID: 29050386
    5. MAOB gene variants are contributing to the etiology of ADHD in the Indo-Caucasoid population PMID: 27341797
    6. This study suggests that MAOB increases ASD risk in males, possibly through its sex-specific regulatory effect on 5-HT metabolism and behavior. PMID: 27381555
    7. These findings suggest an association of platelet MAO-B activity, but a lack of association of MAOB rs1799836 and MAOA-uVNTR, with selected psychotic symptoms in ethnically homogenous veterans with PTSD. PMID: 27112218
    8. No significant association was found between severe agitation and MAOA uVNTR or MAOB rs1799836 polymorphism, revealing that these individual polymorphisms in MAO genes are not related to severe agitation in subjects with schizophrenia and conduct disorder. Higher platelet MAO-B activity was found in subjects with severe agitation vs. non-agitated subjects. Platelet MAO-B activity was not associated with MAOB rs1799836 PMID: 26851573
    9. we performed a meta-analysis in the Chinese population to provide comprehensive data on the association between the MAOB polymorphism and Parkinson's disease. Overall, no significant association was found between the MAOB A644G polymorphism and Parkinson's disease risk in the Chinese population. PMID: 27421021
    10. inhibition of MAO-B using selegiline reversed the cigarette smoke-induced oxidative stress and inflammation in bronchial epithelial cells. PMID: 28108387
    11. Monoamine oxidase B levels are highly expressed in gliomas. PMID: 26689994
    12. The results of this meta-analysis suggest that people in the Asian population with the MAOB intron 13 A allele have an increased risk of Parkinson disease, especially when combined with the COMT LL genotype. PMID: 26000819
    13. Report isolation of MOA-B inhibitors from Vernonia cinerea. PMID: 25857233
    14. MAOB A644G polymorphism was associated with Parkinson Disease risk and a subgroup analyses by ethnicity and gender also found significant relationships between this polymorphism and PD risk. PMID: 24658549
    15. Data (including data from molecular dynamics simulation studies) suggest that the protonation state of the active site residue Lys296 in monoamine oxidase B does not have influence on the hydride transfer reaction in the metabolism of dopamine. PMID: 25220264
    16. Polymorphisms of COMT and MAO-B genes has a unique characteristics among Chinese patients with Parkinson's disease. They may be related with differences in drug response in such patients. PMID: 25636089
    17. There is significantly decreased expression of MAO-A and MAO-B in cancerous tissues when compared with adjacent noncancerous tissues. PMID: 25389533
    18. The results of this study suggested that polymorphisms in MAOB may increase the risk of wearing-off and dyskinesias. PMID: 25034874
    19. study revealed increased platelet MAO-B activity in patients with alcohol dependence; suggests increased platelet MAO-B activity might be used as independent peripheral indicator of alcohol dependence PMID: 25035107
    20. We found significant negative correlations regarding the expression of the genes COMT, MAOB, DRD4, DRD5 and FOS, indicating that increased schizotypy coincides with higher levels of dopaminergic dysregulation on the mRNA-level. PMID: 24630741
    21. Our results confirmed that SNPs in DRD4 and COMT, but not DBH or MAOB are associated with autism and ADHD in small, ethnically homogeneous groups of non-related male Caucasians. PMID: 24210742
    22. genetic variants of COMT, MAOB and DRD2 loci modulate susceptibility to PD in South Indian subjects PMID: 24772965
    23. Data indicate that in vitro MAO-B inhibitory activity by synthesized (coumarin-3-yl)carbamates is comparable with that of the selegiline. PMID: 23474901
    24. The present data suggest that pharmacokinetic or pharmacodynamic factors other than the investigated genetic variants of the MAOB and COMT enzymes seem to determine the response to levodopa in the Iranian PD patients. PMID: 23093014
    25. data may suggest that the MAOB and COMT genetic polymorphisms do not play any role in the pathogenesis of Parkinson's disease in Iranians PMID: 23319194
    26. The rs1799836 SNP did not appear to correlated with attentional bias for facial expressions. PMID: 23054588
    27. results provide additional evidence that GABRB3 and MAOB/NDP gene regions might constitute risk factors for hallucinations and delusions in schizophrenia. PMID: 22414661
    28. G/A dimorphism in intron 13 sequence creates splicing enhancer thus stimulating intron 13 removal efficiency PMID: 22974659
    29. [review] While MAO-B primarily serves in the catabolism of 2-phenylethylamine and contributes to the degradation of other trace amines and dopamine, MAO-A has high affinity for serotonin and norepinephrine. PMID: 21971001
    30. Mao-B platelet protein level may serve as a biomarker for age-related dementia, especially AD. PMID: 22270014
    31. [review] A better understanding of the transcriptional regulation of MAO B may help explain the differential tissue-specific expression of the two isoenzymes and provide insights into the molecular basis of the disorders associated with MAO dysfunction. PMID: 21359973
    32. Results suggest MAOB is a susceptibility gene for schizophrenia in Han Chinese. PMID: 21978760
    33. results demonstrate that the bipartite cavity structure in MAO B plays an important role in substrate and inhibitor recognition to distinguish its specificities from those of MAO A PMID: 21978362
    34. Nitrogen kinetic isotope effects for the oxidation of benzylamine and (1,1-(2)H(2))benzylamine by recombinant human monoamine oxidase B show that cleavage of the CH bond is not concerted with rehybridization of the nitrogen atom. PMID: 21786798
    35. mutation in gene encoding the monoamine degradation enzyme monoamine oxidase B was found in SCZ. PMID: 20479760
    36. COMT rs4680 and MAOB rs1799836 polymorphisms may increase susceptibility to Parkinson's disease risk among Japanese PMID: 21781348
    37. In early stage Alzheimer disease, discrete MAO-B reduction reflects the capacity of the population of MAO-B-positive progenitor cells to adapt to the neurodegenerative process. PMID: 20648649
    38. a monoamine-oxidase epigenetic regulation is involved in effects of smoking PMID: 19956754
    39. Children with different subtypes of ADHD had significantly lower platelet MAO-B activity than control children. PMID: 20022119
    40. Data show that lower p-MAO-B associated with increased inattention scores (Conners' teacher-rating scale) and lower l-MR associated with increased score for oppositional-defiant disorder. PMID: 19863190
    41. first evidence that MAOB polymorphisms influence levels of negative emotionality in healthy human volunteers, may lead to a better understanding of personality traits and susceptibility to developing psychiatric disorders such as major depression PMID: 19657584
    42. Inflammation of the dental pulp was accompanied by a significant decrease in MAO labeling, MAO B (but not MAO A) activity and the increase in SSAO activity. PMID: 19789505
    43. X ray structure to 3 A resolution PMID: 11753429
    44. Association of monoamine oxidase A gene polymorphism with Alzheimer's disease and Lewy body variant. PMID: 12098640
    45. a strong gender difference exists with respect to the modifying effect of MAO-B genotype on the smoking association with parkinson disease PMID: 12428723
    46. MAO-B polymorphisms are associated with smoking behaviour PMID: 12563176
    47. There was no interaction of smoking and the G allele and risk of Parkinson disease. PMID: 12815741
    48. molecular models and binding sites of inhibitors PMID: 12825788
    49. MAO B gene expression is selectively induced by a decreased Sp3/Sp1 ratio and reduced DNA methylation PMID: 12855685
    50. The 1.7-A structure of the reversible isatin-MAO-B complex has been determined; it forms a basis for the interpretation of the enzyme's structure when bound to either reversible or irreversible inhibitors. PMID: 12913124

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  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 数据库链接：

    HGNC: 6834

    OMIM: 309860

    KEGG: hsa:4129

    STRING: 9606.ENSP00000367309

    UniGene: Hs.654473