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  4. LRG1 Recombinant Monoclonal Antibody

LRG1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA229992A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: L02 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
      All lanes: LRG1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 39 kDa
      Observed band size: 39 kDa
    • IHC image of CSB-RA229992A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    LRG1 is a secreted member of the family of leucine-rich repeat (LRR) proteins involved in vascular dysfunction, inflammation, pathological angiogenesis, and fibrosis. Under physiological conditions, LRG1 is constitutively expressed by the liver and granulocytes. LRG1 has been implicated in a variety of cancers, inflammation, pathogenic angiogenesis, lymphocyte differentiation, and neutrophil function. A substantial increase in LRG1 expression has been reported in cancer and diabetes, but also in infections, cardiovascular, kidney, lung, neurological, and autoimmune disorders.

    The production of this recombinant LRG1 antibody was carried out in vitro. It began with immunization of animals so that the B cells could be obtained. The next step was selection of B cells. The positive cells would be screened out for the next step, single B cell antibody sequencing and cloning. Once the LRG1 antibody sequence was obtained, it would be inserted into a plasmid, which could be transfected into mammalian cells for the expression of LRG1 antibody.

  • Uniprot No.：
    P02750
  • 基因名：
  • 别名：
    Leucine-rich alpha-2-glycoprotein (LRG), LRG1, LRG
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human LRG1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    7A1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 基因功能参考文献：
    1. LRG-1 was highly expressed in human retinoblastoma sections, thus providing new insights into the molecular mechanism of retinoblastoma pathogenesis, and suggests a possible new therapeutic target. PMID: 29392314
    2. The detection of LRG1 in accordance with the DeltaRct value (electron-transfer resistance at the electrode surface) of the sensor layer incorporating LRG1 BP3 peptides showed a statistically significant difference (p < 0.001) between adenomas and carcinomas, indicating that the potential use of this biosensing platform for detecting the CRC biomarker, as well as for monitoring the colorectal adenoma-to-carcinoma tra... PMID: 28697446
    3. This study shows that TNF-alpha is the predominant proinflammatory cytokine that induces the secretion of LRG1. LRG1 contributes to angiogenesis-coupled de novo bone formation by increasing angiogenesis and recruiting MSCs in the subchondral bone of osteoarthritis joints. PMID: 28358372
    4. the expression of 3 miRs and 9 mRNAs associate with the PIK3CA status. Expression of LRG1 is independent of luminal (A or B) subtype, decreased after neo-adjuvant aromatase inhibitor treatment. PMID: 27491861
    5. Plasma LRG1 predicts both albuminuria and Chronic kidney disease progression beyond traditional risk factors. It may play a role in the pathologic pathway leading to progression of diabetic kidney disease in T2 Diabetes mellitus. PMID: 28973352
    6. LRG1 may be a key regulatory factor of allergic responses. PMID: 27378305
    7. Results find serum LRG1 levels are significantly associated with LRG1 expression in gastric tumors. Its inhibition suppresses gastric cancer cell growth and invasion suggesting that LRG1 is implicating in gastric tumorigenesis. PMID: 28746773
    8. LRG1 plays a crucial role in the proliferation and apoptosis of colorectal cancer (CRC) by regulating RUNX1 expression. LRG1 may be a potential detection biomarker as well as a marker for monitoring recurrence and therapeutic target for CRC. PMID: 28376129
    9. These findings suggest that sputum LRG is a promising biomarker of local inflammation in asthma. PMID: 27611322
    10. combination of modified whole blood LRG1 mRNA levels, plasma LRG1 protein and Alvarado score at the ED may be useful to diagnose simple and complicated acute appendicitis from other causes of abdominal pain PMID: 28202345
    11. Downregulation of LRG1 is associated with head and neck squamous cell carcinoma. PMID: 28098902
    12. Results suggest that serum LRG-1 is a promising biomarker for pancreatic cancer. PMID: 25058884
    13. Results provided evidence for LRG1 function as a novel inducer of epithelial-mesenchymal transition and angiogenesis in colorectal cancer, which was at least partially through promotion of HIF-1alpha expression. PMID: 26856989
    14. Data show that leucine-rich-alpha-2-glycoprotein 1 (LRG1) is markedly up-regulated and serves as an independent factor of poor outcomes in hepatocellular carcinoma (HCC). PMID: 26517349
    15. Reanalysis of a total of 47 MDS patients revealed biomarker candidates, with alpha-2-HS-glycoprotein and leucine-rich alpha-2-glycoprotein as the most promising candidates. PMID: 26448929
    16. Two of these proteins were verified in a separate patient cohort: values of CRP and LRG1 combined gave a highly significant indication of extended survival post one week of radiotherapy treatment. PMID: 26425690
    17. Findings suggest that LRG1 promotes invasion and migration of glioma cells through TGF-beta signaling pathway. PMID: 26049667
    18. LGR1 is involved in the inhibition of HCC metastasis. PMID: 25814288
    19. silencing the expression of LRG1 suppresses the growth of glioblastoma U251 cells in vitro and in vivo, suggesting that LRG1 may play a critical role in glioblastoma development, and it may have potential clinical implications in glioblastoma therapy. PMID: 25589464
    20. Higher LRG1 is a significant predictor for arterial stiffness, endothelial function, and peripheral arterial disease. PMID: 25636050
    21. The blood proteins IGF1, IGF2, IBP2, IBP3 and A2GL have been proposed as indirect biomarkers for detection of GH administration and as putative biomarkers for breast cancer diagnosis. PMID: 25277046
    22. LRG1 potentially offered clinical value in directing personal treatment for endometrial carcinoma patients PMID: 24760273
    23. Human LRG (hLRG) concentration in the cerebrospinal fluid (CSF) was measured and hLRG expression in post-mortem human cerebral cortex and mouse LRG (mLRG) in the brain were examined. PMID: 24058569
    24. Overexpression of serum LRG1 is associated with non-small cell lung cancer. PMID: 23284758
    25. High Serum leucine-rich alpha-2 glycoprotein is associated with ulcerative colitis. PMID: 22374925
    26. Increased plasma levels of the APC-interacting protein MAPRE1, LRG1, and IGFBP2 precede a diagnosis of colorectal cancer in women PMID: 22277732
    27. This study demonistrated that LRG expression in resident astrocytes increased with age. PMID: 22116432
    28. Screened for NSCLC-related proteins in urinary exosomes by comparing urinary exosomes proteome of normal controls and NSCLC patients. The LRG1 was found to be expressed at higher levels in urinary exosomes and lung tissue of NSCLC patients. PMID: 21557262
    29. LRG1 was identified as a serum biomarker that accurately identifies patients with heart failure. PMID: 21282491
    30. autologous Cyt c is an endogeneous ligand for LRG and PLIbeta and these serum proteins neutralize the autologous Cyt c released from the dead cells PMID: 20442399
    31. cDNA clones for human leucine-rich alpha2-glycoprotein were isolated. LRG expression was up-regulated during neutrophil differentiation but down-regulated during monocytic differentiation. The hLRG gene was localized to chromosome 19p13.3. PMID: 12223515
    32. Mass spectrometry analysis identified the serum inhibitory factor as leucine-rich alpha-2-glycoprotein-1 in enzyme-linked immunosorbent assay . PMID: 16699948
    33. These results also indicate that the CSF level assay of TGF-beta1, TbetaR-II and LRG is useful for the diagnosis of patients with INPH, and TGF-beta1, TbetaR-II and LRG may be involved in the pathogenesis of the disease. PMID: 17194537
    34. Several indirect lines of evidence suggest a role for LRG in implantation/decidualization. PMID: 17583700
    35. These results strongly suggest that LRG was a secretory type 1 acute-phase protein whose expression was up-regulated by the mediator of acute-phase response. PMID: 19324010

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  • 亚细胞定位：
    Secreted.
  • 组织特异性：
    Plasma.
  • 数据库链接：

    HGNC: 29480

    OMIM: 611289

    KEGG: hsa:116844

    STRING: 9606.ENSP00000302621

    UniGene: Hs.655559