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  4. LPAR1 Recombinant Monoclonal Antibody

LPAR1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA922556A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate
      All lanes: EDG2 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42, 42 kDa
      Observed band size: 42 kDa
  • 其他：

产品详情

  • 产品描述：

    LPAR1, also called Edg2, primarily expressed in the brain, can couple to G protein-coupled receptors (GPCRs) and participate in regulating cell proliferation and survival, migration, cell-cell contact, cytoskeletal alterations, calcium mobilization, adenylyl acylase inhibition, and apoptosis after being stimulated by LPA. LPAR1 deletion causes neurodevelopmental disorders and CNS diseases, such as brain cancer, neuropsychiatric disorders, demyelination diseases, and neuropathic pain.  LPAR1 might play a vital role in various benign and malignant diseases by activating the PI3K/AKT pathway.

    The production of this recombinant LPAR1 antibody started with immunization. And then the workflow included B cell harvest and enrichment; import single B cell; assays to identify the specificity, affinity & functionality of the cell; export the single B cell; cDNA synthesis and sequencing; express the LPAR1 antibody in mammalian cells. The target LPAR1 antibody was validated in ELISA, WB.

  • Uniprot No.：
    Q92633
  • 基因名：
  • 别名：
    LPAR1; EDG2; LPA1; Lysophosphatidic acid receptor 1; LPA receptor 1; LPA-1; Lysophosphatidic acid receptor Edg-2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human EDG2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4C3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor for lysophosphatidic acid (LPA). Plays a role in the reorganization of the actin cytoskeleton, cell migration, differentiation and proliferation, and thereby contributes to the responses to tissue damage and infectious agents. Activates downstream signaling cascades via the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Signaling inhibits adenylyl cyclase activity and decreases cellular cAMP levels. Signaling triggers an increase of cytoplasmic Ca(2+) levels. Activates RALA; this leads to the activation of phospholipase C (PLC) and the formation of inositol 1,4,5-trisphosphate. Signaling mediates activation of down-stream MAP kinases. Contributes to the regulation of cell shape. Promotes Rho-dependent reorganization of the actin cytoskeleton in neuronal cells and neurite retraction. Promotes the activation of Rho and the formation of actin stress fibers. Promotes formation of lamellipodia at the leading edge of migrating cells via activation of RAC1. Through its function as lysophosphatidic acid receptor, plays a role in chemotaxis and cell migration, including responses to injury and wounding. Plays a role in triggering inflammation in response to bacterial lipopolysaccharide (LPS) via its interaction with CD14. Promotes cell proliferation in response to lysophosphatidic acid. Required for normal skeleton development. May play a role in osteoblast differentiation. Required for normal brain development. Required for normal proliferation, survival and maturation of newly formed neurons in the adult dentate gyrus. Plays a role in pain perception and in the initiation of neuropathic pain.
  • 基因功能参考文献：
    1. ATX-LPA axis facilitates estrogen-induced endometrial cancer cell proliferation via MAPK/ERK signaling pathway. PMID: 29328374
    2. DLD-C-F cells formed large-sized colonies, but not DLD-F-C cells, correlating with LPAR1 and LPAR6 gene expression levels. These results suggest that LPA1 and LPA6 may regulate the colony formation activity in DLD1 cells treated with anticancer drugs. PMID: 29369010
    3. endogenous LPA1 receptor signaling and regulation PMID: 28943105
    4. The increased expression of LPA and LPAR1 is associated with the fibrosis and hypertrophy of the ligamentum flavum in patients with Lumbar Spinal Stenosis. PMID: 28323698
    5. myeloma cells stimulate mesenchymal stem cells (MSCs to produce autotaxin, an indispensable enzyme for the biosynthesis of lysophosphatidic acid, and LPA receptor 1 (LPA1) and 3 (LPA3) transduce opposite signals to MSCs to determine the fate of MSCs. PMID: 27641212
    6. LPA1 plays a critical role in EGF responses and that FFA4 agonists inhibit proliferation by suppressing positive cross-talk between LPA1 and the EGF receptor PMID: 27474750
    7. ADAMTS7 and LPA single nucleotide polymorphisms are related to a 24-h ambulatory systolic-diastolic pressure regression index. PMID: 28092973
    8. Polymorphism rs7023923 located near LPAR1 gene: the association of rs7023923 with monocytosis was confirmed among healthy blood donors (p = 0.0156) but not among patients admitted for elective coronarography (p = 0.61). PMID: 25563464
    9. These results suggest that autotaxin-LPA-LPA receptor 1-AKT1 signaling axis is critical for maintaining Cancer stem cells(CSC) characteristics through an autocrine loop and provide a novel therapeutic target for ovarian CSCs PMID: 26800320
    10. LPA-type agonist, via Carbonyl-oxygen/Lys39 interaction facilitates the formation of a hypothetical N-terminal cap tightly packed over the LPA1 heptahelical bundle. PMID: 26268898
    11. Data show high expression levels of LPAR2 and LPAR1 in endometrial cancer tissue with positive correlations with FIGO stage suggesting them as potential biomarkers for endometrial cancer progression. PMID: 26327335
    12. Data suggest LPA (lysophosphatidic acid; acting via LPAR1) and endothelin activate Cdc42, concurrent with biphasic decrease in Rac1 activity, differential effects on RhoA; LPA/endothelin stim. leads to remodeling of invadosomes in melanoma cells. PMID: 26740622
    13. we show that LPAR1 is a novel susceptibility gene for human essential hypertension and that stress, such as shortage of sleep, increases the susceptibility of patients with risk allele to essential hypertension. PMID: 26123684
    14. Study presents three crystal structures of LPA1 in complex with antagonist tool compounds selected and designed through structural and stability analyses. PMID: 26091040
    15. Results indicate that lysophosphatidic acid-lysophosphatidic acid receptor 1 (LPA-LPA1) signaling contributes to the activation of rheumatoid arthritis fibroblast-like synoviocytes (FLSs). PMID: 25273676
    16. Lysophosphatidylethanolamine utilizes LPA(1) and CD97 in a breast cancer cell line. PMID: 23838008
    17. LPA1 and LPA2 are major LPA receptor subtypes compared with low-expressed LPA3 in PANC-1 tumor cells. PMID: 24061591
    18. Lysophosphatidic acid (LPA) increased hepatocellular carcinoma cells cell invasion, which was LPA-receptor dependent. PMID: 23569130
    19. This review aims to characterize gintonin as an LPA receptor ligand and lists the advantages of LPA-ginseng protein complexes over free LPAs. PMID: 23017203
    20. study identified a novel role of TGFbeta in the control of LPA1 expression and LPA1-coupled biological functions, adding LPA1 to the list of TGFbeta-repressed target genes PMID: 22824789
    21. CD97 expression in human thyroid cancers correlated with LPA receptor and markers of aggressiveness including Ki67 and pAKT. PMID: 22797060
    22. a crosslink between Egr-1 and periostin in cancer cells PMID: 22659570
    23. The ability of LPA-LPA(1) signaling to promote epithelial cell apoptosis and fibroblast resistance to apoptosis may therefore contribute to the capacity of this signaling pathway to regulate the development of pulmonary fibrosis after lung injury. PMID: 22021336
    24. CD97 functioned to mediate invasion in prostate cancer cells, by associating with lysophosphatidic acid receptor 1 (LPAR1), leading to enhanced LPA-dependent RHO and extracellular signal-regulated kinase activation. PMID: 21978933
    25. the ATX-LPA-LPAR axis is a critical regulator of embryonic vascular development that is conserved in vertebrates PMID: 21971049
    26. This work shows for the first time that key components of the LPA pathway are modulated following traumatic brain injuries in humans. PMID: 21234797
    27. Mutation in LPA1 gene indicate that alteration in LPA receptor gene may play some role in the pathogenesis in human osteosarcoma cells. PMID: 21116120
    28. LPA1 receptor has a role in angiogenesis in tumor cells and xenografts PMID: 20708100
    29. Data show that CLL cells express LPA receptors LPA(1-5) and VEGF receptors, and the plasma levels of VEGF are elevated in CLL patients. PMID: 19860625
    30. show that human microglia express LPA receptor subtypes LPA(1), LPA(2), and LPA(3) on mRNA and protein level. LPA activation of C13NJ cells induced Rho and extracellular signal-regulated kinase activation and enhanced cellular ATP production. PMID: 19899077
    31. LP(A1) and myelin basic protein colocalized in brain, but oligodendrocyte soma showed stronger signals for LP(A1) than myelinated fibers, whereas the reverse was true for myelin basic protein so LP(A1) may be involved in myelin formation or maintenance. PMID: 11948806
    32. demonstrate that two biological fluids, blood plasma and seminal plasma, differentially activate LPA receptors PMID: 12123830
    33. Data suggest that LPA(1) receptors couple to a G(i)-phosphoinositide 3-kinase-Tiam1 pathway to activate Rac, with consequent suppression of RhoA activity, and thereby stimulate cell spreading and motility. PMID: 12393875
    34. lysophosphatidic acid-coupled LPA1/EDG-2 receptors are endocytosed via a dynamin2- and Rab5-dependent pathway PMID: 12668728
    35. LPA acts as a potent stimulator of colon cancer progression, although the binding to LPA1 and LPA2 induces slightly different responses. PMID: 12670925
    36. LPA stimulation promotes the interaction of the LPA(2) receptor with a focal adhesion molecule, TRIP6 PMID: 14688263
    37. EDG-2 expression was increased in low-grade adenoma compared with that in normal mucosa (P < 0.001). EDG-2 expression was significantly greater in adenomas with larger diameters (P < 0.001). PMID: 14696401
    38. Amyloid beta-protein stimulated in monocytes the gene expression for sphingosine-1-phosphate receptor 2, which is amyloid beta-protein-induced migration. PMID: 15208267
    39. data suggest that endothelial differentiation gene EDG-7 and EDG-2 lysophosphatidic acid receptors play a diverse role in mesangial cell proliferation PMID: 15292052
    40. formation of the LPA receptor/PDZ domain-containing RhoGEF complex plays a pivotal role in LPA-induced RhoA activation PMID: 15755723
    41. We show that in addition to promoting LPA(1) signaling, membrane cholesterol is essential for the association of LPA(1) with beta-arrestin, which leads to signal attenuation and clathrin-dependent endocytosis of LPA(1). PMID: 16263766
    42. These findings demonstrate that trafficking of LPA1 to the nucleus is influenced by cell-matrix interactions and that nuclear LPA1 may be involved in regulating intranuclear protein phosphorylation and signalling. PMID: 16716145
    43. LPA(1) transduces Galphai-dependent signals to promote nuclear localization of androgen receptor and cell proliferation PMID: 16809448
    44. EDG2 and EDG4 cooperate to promote LPA-stimulated chemotaxis in breast tumor cell lines. PMID: 17496233
    45. expression of LPA-induced inflammatory response genes is mediated by LPA1 and LPA3 PMID: 17923111
    46. Lysophosphatidic acid receptor 1 may contribute to the pathogenesis of rheumatoid arthritis through the modulation of fibroblast-like synoviocyte migration and cytokine production. PMID: 18006645
    47. distinct molecular mechanisms regulate agonist-dependent and PMA-dependent internalization of the LPA 1 receptor. PMID: 18089565
    48. Down-regulation of EDG2 is functionally important to suppression of tumor metastasis in breast neoplasms. PMID: 18089805
    49. Through the stepwise association study, an SNP located in the promoter region of EDG2 (-2,820G/A; rs10980705) showed significant association with knee osteoarthritis in two independent populations. PMID: 18325907
    50. LPA may play a role in angiogenesis of endometrium and placenta through induction of IL-8 in endometrial stromal cells during pregnancy. PMID: 18617617

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  • 亚细胞定位：
    Cell surface. Cell membrane; Multi-pass membrane protein. Endosome.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes. Detected in l
  • 数据库链接：

    HGNC: 3166

    OMIM: 602282

    KEGG: hsa:1902

    STRING: 9606.ENSP00000351755

    UniGene: Hs.126667