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  4. KDM1A Recombinant Monoclonal Antibody

KDM1A Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA222329A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate
      All lanes: KDM1A antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 93, 96 kDa
      Observed band size: 110 kDa
    • IHC image of CSB-RA222329A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA222329A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    The recombinant KDM1A antibody was prepared by obtaining the antibody genes, cloning the genes into a plasma vector to construct vector clone, transfecting the vector clone into a mammalian cell line for transient expression, and purifying the antibody by affinity-chromatography. This recombinant KDM1A antibody has been verified to detect the KDM1A protein from Human in the ELISA, WB, IHC.

    KDM1A is a histone demethylase enzyme responsible for the removal of specific methyl motifs from certain histone proteins. KDM1A plays significant roles in modulating embryonic development, differentiation, carcinogenesis, and neuronal function. KDM1A is involved in the development of various pathological conditions, such as cancer, neuronal disorders, and viral infections. Upregulation of KDM1A has been associated with poor prognosis in a variety of cancer types. KDM1A participates in maintaining stemness, regulating hypoxia, epithelial-to-mesenchymal transition (EMT), and evading immune surveillance of cancer cells.

  • Uniprot No.：
    O60341
  • 基因名：
    KDM1A
  • 别名：
    Lysine-specific histone demethylase 1A (EC 1.-.-.-) (BRAF35-HDAC complex protein BHC110) (Flavin-containing amine oxidase domain-containing protein 2), KDM1A, AOF2 KDM1 KIAA0601 LSD1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human KDM1 / LSD1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1G7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
  • 基因功能参考文献：
    1. the role of the LSD1 complex in the reactivation of HIV-1 transcription from latency PMID: 30105631
    2. LSD1 and JMJD2C disable oncogenic Ras- or Braf-induced senescence by enabling the expression of E2F target genes PMID: 29438700
    3. LSD1 inhibition may serve as a promising epigenetic adjuvant therapy to subvert its pleiotropic roles in breast cancer progression and treatment resistance. PMID: 29311580
    4. Results show that KDM1A is upregulated in the gastric cancer (GC) cells and tissues. Its expression is negatively modulated by mir329 which directly targets its 3'utr. PMID: 29130516
    5. FOXP4-AS1 is overexpressed in osteosarcoma (OS), and is the independent risk factor in OS prognosis. Upregulated FOXP4-AS1 promotes the proliferation, migration and cell cycle, but inhibits apoptosis of OS cells. Furthermore, FOXP4-AS1 participates in the development and progression of OS by downregulating LATS1 via binding to LSD1 and EZH2. PMID: 29859193
    6. Enhancer of Zeste Homolog 2 (EZH2), SET domain, bifurcated 1 protein (SETDB1), lysine-specific histone demethylase 1 (LSD1), histone H3 methylation (H3K9me3 and H3K27me3) expression are altered in colorectal cancer (CRC) and may play a role in colorectal carcinogenesis. PMID: 30105513
    7. the crystal structure of LSD1 in complex with the inhibitor 4-[5-(Piperidin-4-ylmethoxy)-2-(p-tolyl)pyridin-3-yl]benzonitrile at 2.96 A, is reported. PMID: 29949906
    8. This study showed that tetrahydrofolate stably binds to the LSD1/CoREST complex, in its open conformation, at its entrance. PMID: 29161028
    9. Study reveals LSD1 as a molecular rheostat selectively regulating H3K9 demethylation at cell cycle gene loci, thereby representing a key player in oncogenesis and a viable target for cancer therapy. PMID: 28991226
    10. KDM1A is a unique epigenetic modifier with the ability to maintain interactions with a variety of different protein complexes, noncoding RNAs, microRNAs, and transcription factors. The functional significance of KDM1A is maintained by its interactions at multiple sites in the genome, particularly its binding to promoters and enhancers. [review] PMID: 29921310
    11. our findings identified LSD1 as a novel negative regulator of autophagy through the mTOR signaling pathway in ovarian cancer HO8910 cells and indicated that LSD1 may function as a driving factor of ovarian cancer progression via deregulating autophagy. PMID: 29749504
    12. a small-molecule LSD1 inhibitor-SP-2509-blocks important demethylase-independent functions and suppresses castration-resistant prostate cancer cell viability demonstrates the potential of LSD1 inhibition in this disease. PMID: 29581250
    13. expression of the nearby cyclindependent kinase inhibitor 1C (CDKN1C) gene was revealed to be upregulated after SP3 knockdown in cells that possessed non-risk alleles. This suggests that CDKN1C is potentially one of the functional targets of SNP rs163184, which modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a PMID: 29207083
    14. Silencing of LSD1 is capable of removing the mono- and dimethyl groups from H3K4, and upregulating the histone acetylation of H3 in JeKo1 and MOLT4 cells. The silencing of LSD1 inhibited cell growth and induced cell apoptosis. PMID: 28627608
    15. LSD1 and ERRalpha coregulate several target genes involved in cell migration, including the MMP1 matrix metallo-protease, also activated through H3K9 demethylation at the transcriptional start site. PMID: 28348226
    16. concomitant LSD1 and HDAC inhibition synergistically induces cell death in rhabdomyosarcoma cells by shifting the ratio of pro- and antiapoptotic BCL-2 proteins in favor of apoptosis, thereby engaging the intrinsic apoptotic pathway PMID: 28617441
    17. Results show that LSD1 protein level is elevated in prostate tumors (PCa) and correlate with faster tumor growth in xenograft mouse models. Knockdown of LSD1 reduces PCa cell viability, and gene expression data suggest a dual coregulatory role of LSD1 for VDR, acting as a coactivator and corepressor in a locus-specific manner. PMID: 28811844
    18. Study showed that repressed HBV cccDNA chromatin state is activated by LSD1 by demethylating H3K9. Also, LSD1 was shown to be recruited to viral promoters in an HBx dependent manner. PMID: 27174370
    19. LSD1 knockdown has a broad effect on histone lysine methylation. PMID: 28601046
    20. analysis of a novel cellular stress response mechanism in cancer cells and a key role of LSD1/SIRT1/KU70 dynamic interaction in regulating DNA repair and mutation acquisition PMID: 27384990
    21. Patients with mutations 6 showed higher rate of achieving major molecular response than those<6 (P=0.0381). Mutations in epigenetic regulator, ASXL1, TET2, TET3, KDM1A and MSH6 were found in 25% of patients. TET2 or TET3, AKT1 and RUNX1 were mutated in one patient each. ASXL1 was mutated within exon 12 in three cases PMID: 28452984
    22. Study demonstrated that LSD1 in cooperation with MYCN controls cell migration and invasiveness of neuroblastoma cells through transcription regulation of the metastatic suppressor NDRG1. PMID: 27894074
    23. Results show that LSD1 could directly bind to the promoter of P21, inducing H3K4me2 demethylation. PMID: 28209170
    24. The autophagy induced by 2-PCPA requires LC3-II processing machinery..2-PCPA treatment induces the change of global gene expression program, including a series of autophagy-related genes, such as SQSTM1/p62. Taken together, our data indicate that KDM1A/LSD1 inhibitors induce autophagy through affecting the expression of autophagy-related genes and in a BECN1-independent manner PMID: 28800922
    25. LSD1 is specifically mislocalized to pathological protein aggregates in the Alzheimer's disease and frontotemporal dementia patients brain. PMID: 28993646
    26. these results demonstrate crosstalk between the lysine demethylase KDM1A and the DNA methyltransferase DNMT1, which could be involved in carcinogenesis independently of its role in DNA methylation PMID: 27449289
    27. LSD1 inhibition with HCI-2509 decreases the c-MYC level in poorly differentiated prostate cancer cell lines. PMID: 27349498
    28. Such a relatively simple strategy uncovered two new classes of LSD1 inhibitors , which reveal unexpected binding modes at the same protein surface, establishing specific and common contacts, and highlight novel routes for the development of compounds targeting epigenetic processes. PMID: 27626075
    29. we show that KDM1A promotes cancer metastasis in non-small cell lung cancer cells by repressing TIMP3 (tissue inhibitor of metalloproteinase 3) expression. PMID: 27058897
    30. Results show that LSD1 expression was upregulated in breast carcinoma and, induced EMT in breast cancer cells through demethylation of H3K4me at the E-cadherin promoter. Its phosphorylation at Ser112 is crucial for these functions. PMID: 27572339
    31. LSD1 directly associates with the promoter of the HEYL gene. PMID: 27018646
    32. LSD1 controls autophagy in neuroblastoma cells through SESN2 transcription regulation. PMID: 28783174
    33. Mechanistic investigations showed that LINC01133 could interact with EZH2, LSD1 and recruit them to KLF2, P21 or E-cadherin promoter regions to repress their transcription. PMID: 26840083
    34. estrogen induced TFPI-2 expression in MCF7 cells is mediated by ERalpha and also by the action of LSD1. PMID: 28088469
    35. CENPE was regulated by the co-binding of LSD1 and AR to its promoter, which was associated with loss of RB1 in CRPC. PMID: 28916652
    36. RBPJ interacts with L3MBTL3 to promote repression of Notch signaling via histone demethylase KDM1A. PMID: 29030483
    37. High LSD1 expression is associated with small-cell lung cancer. PMID: 28667074
    38. The role of LSD1 in the EMT process. PMID: 28720390
    39. p53-CTD inhibits LSD1 activity and that the direct association between the two proteins can contribute to their functional cross-talk. PMID: 28784588
    40. LSD1 is a key regulator of HIF1alpha/VEGF-mediated tumor angiogenesis by antagonizing the crosstalk between PTMs involving HIF1alpha protein degradation. PMID: 28534506
    41. This study identified histone modification by SUMO as the first post-translational modification that stimulates intranucleosomal demethylation by the developmentally critical LSD1-CoREST complex. PMID: 28832116
    42. These findings provide insight into the regulation of LSD1 and Epithelial-to-Mesenchymal Transition (EMT) and identify MOF as a critical suppressor of EMT and tumor progression. PMID: 27292636
    43. High LSD1 expression is associated with colon cancer. PMID: 28121627
    44. results demonstrate that CoREST and LSD1 downregulate the Notch pathway in the developing cerebral cortex PMID: 27112428
    45. LSD1 is a primary and positive regulator of the HPV16 E7-induced epithelial-mesenchymal transition in cervical cancer PMID: 27894088
    46. KDM1A inhibitors may be utilized to alleviate acquired resistance to sorafenib in hepatocellular carcinoma cells. PMID: 28377178
    47. This study identified the lysine-specific demethylase KDM1A as a novel interaction partner of ZEB2 and demonstrated that mouse and human T-cell acute lymphoblastic leukemia cells with increased ZEB2 levels critically depend on KDM1A activity for survival. PMID: 28069602
    48. LSD1 overexpression is associated with non-small cell lung cancer. PMID: 28223039
    49. a model in which the EZH2/HOXA11-AS/LSD1 complex and HOXA11-AS/miR-1297/EZH2 cross-talk serve as critical effectors in gastric cancer tumorigenesis and progression. PMID: 27651312
    50. these results demonstrate the relevance of LSD1 in gene regulation and how even moderate alterations in its stability, catalytic activity and binding properties can strongly affect organism development. PMID: 27094131

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  • 相关疾病：
    Cleft palate, psychomotor retardation, and distinctive facial features (CPRF)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    HGNC: 29079

    OMIM: 609132

    KEGG: hsa:23028

    STRING: 9606.ENSP00000383042

    UniGene: Hs.591518