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  4. ILF3 Recombinant Monoclonal Antibody

ILF3 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA289890A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA289890A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA289890A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela Cells with CSB-RA289890A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    ILF3 encodes a double-stranded RNA (dsRNA)-binding protein that complexes with other proteins, mRNAs, small noncoding RNAs, and dsRNAs to regulate gene expression and stabilize mRNAs. Through binding to different cellular RNAs, ILF3 participates in diverse cellular functions such as mRNA stabilization, translation inhibition, and noncoding RNA biogenesis. ILF3 is responsible for hyperlipidemia-induced arteriosclerotic calcification by mediating BMP2 and STAT1 transcription. Abnormal expression of ILF3 has been identified in a number of malignancies, and ILF3 has been reported to be involved in tumor proliferation, invasion, and metastasis.

    This recombinant ILF3 antibody was developed with the Single B cell platform. The main process included identification and isolation of single B cells; amplification and cloning of ILF3 antibody gene; expression, screening, and identification of antibody specificity.  And this ILF3 antibody has been validated in ELISA, IHC, IF.

  • Uniprot No.：
    Q12906
  • 基因名：
    ILF3
  • 别名：
    Interleukin enhancer-binding factor 3 (Double-stranded RNA-binding protein 76) (DRBP76) (M-phase phosphoprotein 4) (MPP4) (Nuclear factor associated with dsRNA) (NFAR) (Nuclear factor of activated T-cells 90 kDa) (NF-AT-90) (Translational control protein 80) (TCP80), ILF3, DRBF MPHOSPH4 NF90
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human ILF3
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    7H8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs. As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response. Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs.
  • 基因功能参考文献：
    1. DNA- and RNA-binding protein NF90/ILF3/NF110 associates with chromatin to hierarchically regulate transcription factors that promote proliferation and suppress differentiation. PMID: 29590119
    2. Reprot a coordinated regulation of circRNA biogenesis and function by NF90/NF110 in viral infection. PMID: 28625552
    3. The NF90 regulates PARP1 mRNA stability in hepatocellular carcinoma cells, and NF90 is a potential target to inhibit PARP1 activity. PMID: 28487110
    4. Variants in ILF3 gene is associated with thrombosis. PMID: 26908601
    5. these data establish miR-590-5p as an anti-onco-miR that inhibits colorectal cancer angiogenesis and metastasis through a new mechanism involving NF90/VEGFA signaling axis. PMID: 27735951
    6. NF90 exerts its antiviral activity by antagonizing the inhibitory role of NS1 on PKR phosphorylation PMID: 27423063
    7. These data suggest that, like ADAR2, underlying sequences in dsRNA may influence how NF90 recognizes its target RNAs PMID: 26712564
    8. The NF90 C-terminus is involved in conformational changes in the protein after RNA binding. PMID: 26795062
    9. Taken together, our discovery of the function of TCP80 and RHA in regulating p53 IRES and p53 induction following DNA damage provides a better understanding of the mechanisms that regulate IRES-mediated p53 translation in response to genotoxic stress. PMID: 26273641
    10. data suggest that lncRNA SALNR modulates cellular senescence at least partly through changing NF90 activity PMID: 26491010
    11. Data indicate the role of cyclin-dependent kinase inhibitor 1A (p21) and interleukin enhancer binding factor 3 (ILF3) pathway in mediating the anti-leukemic activity of sodium dichloroacetate (DCA) in oncoprotein p53null leukemic cells. PMID: 25544776
    12. Suppression of NF90 caused a decrease in the half-life of cyclin E1 mRNA PMID: 25399696
    13. the role of the human NF90 protein isoforms in DNA and RNA virus replication PMID: 25447144
    14. NF90 exerts antiviral activity through regulation of PKR phosphorylation and stress granules in infected cells. PMID: 24623135
    15. The function of NF90 in posttranscriptional gene regulation and microRNA biogenesis. [Review] PMID: 23965975
    16. Data indicate that knockdown of interleukin enhancer-binding factor 3 (ILF3) leads to the increased levels of mature urokinase-type plasminogen activator (uPA) mRNA-targeting miRNAs miR-193a, miR-193b and miR-181a. PMID: 22986534
    17. contributes to maintaining low levels of senescence-associated secretory phenotype factors in non-senescent cells PMID: 23117626
    18. The unique C-terminal region of ILF3/NF110 is important for promoting survivin expression and for high affinity binding to YM155. PMID: 22442257
    19. high expression of ERCC1, XPB and ILF3 was observed in human epithelial ovarian cancer PMID: 21971700
    20. These data suggest that DRBP76, via its association with VP35, exerts an anti-Ebola virus function. PMID: 21987769
    21. The protein polymorphism of Ilf3/NF90 and the various subcellular localizations of their isoforms may partially explain the various functions previously reported for these proteins. PMID: 21811582
    22. associations of rs2569512 of ILF3 to myocardial infarction among individuals stratified by the absence or presence of hypertension, diabetes mellitus and chronic kidney disease PMID: 21347509
    23. Production of HIV particles is regulated by altering sub-cellular localization and dynamics of Rev induced by double-strand RNA binding protein. PMID: 21364984
    24. NF90 binds the dengue virus RNA 3' terminus and is a positive regulator of dengue virus. PMID: 21386893
    25. The relationship between rs6929846 of BTN2A1 or rs2569512 of ILF3 and myocardial infarction is influenced by the serum concentrations of high density lipoprotein cholesterol and low density lipoprotein cholesterol, respectively. PMID: 21468600
    26. Data suggest that physiologic DRBP76 expression, isoform distribution and subcellular localization are profoundly altered upon malignant transformation. PMID: 20668518
    27. Nuclear export of this protein is required for interleukin-2 mRNA stabilization. PMID: 12504009
    28. Results describe the expression of four isoforms of nuclear factor 90, including NF110, by cDNA cloning and mass spectrometric analysis of proteins isolated from human cells PMID: 12946349
    29. nuclear export of ILF3 is mediated by exportin-5 and minihelix-containing RNA PMID: 14570900
    30. the multiple cellular functions, i.e., translation control, interleukin-2 enhancer binding, or cell division, of ILF3 are fulfilled by alternatively spliced isoforms. PMID: 14654356
    31. NF90 and NF110 engage RNA differentially and translocate from the nucleus to the cytoplasm in mitosis PMID: 15811368
    32. ADAR1 has the potential both to change information content through editing of mRNA and to regulate gene expression through interacting with NF90 PMID: 16055709
    33. The human Nuclear Factor 90 c-terminal variant protein encoded by ILF3 interacts with Rev to inhibit Rev-mediated RNA transport. PMID: 17125513
    34. Our results show that perturbation Tat/TAR RNA interaction by the dsRNA binding nuclear factor 90 protein is sufficient to inhibit transcriptional activation of HIV-1. PMID: 17565699
    35. DRBP76/NF90 isoform facilitates VEGF expression by promoting VEGF mRNA loading onto polysomes PMID: 18039850
    36. NFARs exert influence on mRNA trafficking and the modulation of translation rates and may constitute an innate immune translational surveillance mechanism important in host defense countermeasures against virus infection PMID: 18337511
    37. MKP-1 upregulation by oxidative stress is potently influenced by increased mRNA stability and translation, mediated at least in part by the RNA-binding proteins HuR and NF90. PMID: 18490444
    38. ILF-3, which has been known to regulate IL-2 expression in T cells, up-regulates synoviolin expression with GABPalpha in rheumatoid synovial cells PMID: 19116932
    39. NF90 inhibits influenza virus replication during the early phase of infection through direct interaction with viral NP. PMID: 19494010

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  • 亚细胞定位：
    Nucleus, nucleolus. Cytoplasm. Nucleus.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    HGNC: 6038

    OMIM: 603182

    KEGG: hsa:3609

    STRING: 9606.ENSP00000404121

    UniGene: Hs.465885