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  4. Histone H2B type 1-K Recombinant Monoclonal Antibody

Histone H2B type 1-K Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA010411A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Mouse brain tissue, Rat liver tissue, Rat lung tissue
      All lanes: Histone H2B type 1-K antibody at 0.16μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 14 KDa
      Observed band size: 14 KDa
    • IHC image of CSB-RA010411A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品描述：

    CUSABIO got the DNA sequence of the histone H2B type 1-K monoclonal antibody that was produced from the splenocytes derived from animals with the human histone H2B type 1-K synthesized peptide immunization. The DNA sequence was cloned into the plasmid and then transfected into cell lines for in vitro expression. The product is the histone H2B type 1-K recombinant monoclonal antibody. It is a rabbit IgG antibody purified using the affinity-chromatography method. This histone H2B type 1-K antibody is recommended for ELISA, WB, and IHC applications and detects the histone H2B type 1-K protein from human sources.

    A study by Shih-Yi Lin et al. has reported that Alpha 1-Antitrypsin and H2B1K are diagnostic and prognostic biomarkers for urothelial carcinoma after proteome profiling of urinary exosomes. H2B1K has been demonstrated to have prognostic significance, as 3-fold increases in its levels properly predicted recurrence.

  • Uniprot No.：
    O60814
  • 基因名：
    HIST1H2BK
  • 别名：
    H2BC12 antibody; H2BFT antibody; HIRIP1 antibody; HIST1H2BK antibody; Histone H2B type 1-K antibody; H2B K antibody; HIRA-interacting protein 1 antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4G10
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:5000-1:10000
    IHC 1:50-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.; Has broad antibacterial activity. May contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid.
  • 基因功能参考文献：
    1. Histone H2B antimicrobial peptides exhibit antibacterial activity and contribute to the formation of the antimicrobial barrier of the colonic epithelium PMID: 15019208
  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    Histone H2B family
  • 数据库链接：

    HGNC: 13954

    OMIM: 615045

    KEGG: hsa:85236

    STRING: 9606.ENSP00000349430

    UniGene: Hs.437275