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  4. HNRNPK Recombinant Monoclonal Antibody

HNRNPK Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA010611A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate
      All lanes: HNRNPK antibody at 1.3μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 51, 52, 49 KDa
      Observed band size: 60 KDa
    • IHC image of CSB-RA010611A0HU diluted at 1:130.5 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA010611A0HU diluted at 1:130.5 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of A549 cells with CSB-RA010611A0HU at 1:43.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
    • Immunoprecipitating HNRNPK in HepG2 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-RA010611A0HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-RA010611A0HU (3μg) + HepG2 whole cell lysate (500μg)
      Lane 3: HepG2 whole cell lysate (20μg)

  • 其他：

产品详情

  • 产品描述：

    The recombinant HNRNPK antibody production commenced with the obtaining of genes encoding antibody against HNRNPK. Antibody genes were obtained by sequencing and screening DNA reversely transcribed from RNA that was extracted from the B cells isolated from immunized animals. These genes were cloned into plasma vectors and subsequently transfected into a mammalian cell line for production. The product is the recombinant HNRNPK antibody. It underwent purification using affinity-chromatography from the cell culture medium. This recombinant HNRNPK antibody has been validated to detect the HNRNPK protein from Human in the ELISA, WB, IHC, IF, IP.

    HNRNPK is a DNA/RNA-binding protein involved in multiple biological processes including the regulation of gene transcription, pre-mRNA splicing, mRNA nuclear export, mRNA translation, RNA stability, chromatin remodeling, and decay. Dysregulation of HNRNPK is related to carcinogenesis, cancer development, progression, and prognosis. In some malignancies, HNRNPK is linked to dismal prognosis. In addition to oncogenic functions, HNRNPK, ad a transcriptional co-activator of p53, may act as a tumor suppressor and loss of HNRNPK was prone to cause tumorigenesis.

  • Uniprot No.：
    P61978
  • 基因名：
    HNRNPK
  • 别名：
    CSBP antibody; dC stretch binding protein antibody; FLJ41122 antibody; Heterogeneous nuclear ribonucleoprotein K antibody; hnRNP K antibody; HNRNPK antibody; HNRPK antibody; HNRPK_HUMAN antibody; Transformation up regulated nuclear protein antibody; Transformation up-regulated nuclear protein antibody; Transformation upregulated nuclear protein antibody; TUNP antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human HNRNPK
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3G7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
    IP 1:200-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.
  • 基因功能参考文献：
    1. Data show that heterogeneous nuclear ribonucleoprotein K (hnRNP K) and influenza virus NS1A binding protein (NS1-BP) regulate host splicing events and that viral infection causes mis-splicing of some of these transcripts. PMID: 29921878
    2. Study findings underscore the biological significance of MRPL33-L and hnRNPK in the tumor formation and identifies hnRNPK as a critical splicing regulator of MRPL33 pre-mRNA in cancer cells. PMID: 28869607
    3. hnRNPK regulates PLK1 expression by competing with the PLK1-targeting miRNAs, miR-149-3p and miR-193b-5p. PMID: 28708135
    4. we discovered that hnRNPK plays an important role in bladder cancer, suggesting that it is a potential prognostic marker and a promising target for treating bladder cancer. PMID: 27862976
    5. The hnRNPK positively regulates the level of prostate tumor overexpressed 1-antisense 1 which harbors five binding sites for miR-1207-5p. The knockdown of hnRNPK or PTOV1-AS1 increased the enrichment of heme oxygenase-1 mRNA in miR-1207-5p-mediated miRNA-induced silencing complex and thus suppressed the expression of heme oxygenase-1. PMID: 28228215
    6. High HNRNPK expression is associated with pancreatic cancer. PMID: 28423622
    7. Down-regulation of DAB2IP correlated negatively with hnRNPK and MMP2 expressions in CRC tissues. In conclusion, our study elucidates a novel mechanism of the DAB2IP/hnRNPK/MMP2 axis in the regulation of CRC invasion and metastasis, which may be a potential therapeutic target. PMID: 28335083
    8. Tumor cells bearing a p53 mutation showed increased damage levels and delayed repair. Knockdown of hnRNPK applied simultaneously with irradiation reduced colony-forming ability and survival of tumor cells. Taken together, our data shows that hnRNPK is a relevant modifier of DNA damage repair and tumor cell survival. We therefore recommend further studies to evaluate the potential of hnRNPK as a drug target for improvement PMID: 28426877
    9. Data suggest that hnRNPK plays role in heat shock response of cells by regulating HSF1; hnRNPK inhibits HSF1 activity, resulting in reduced expression of HSP27 and HSP70 mRNAs; hnRNPK also down-regulates binding of HSF1 to heat shock response element. (hnRNPK = heterogeneous-nuclear ribonucleoprotein K; HSF1 = heat shock transcription factor 1; HSP = heat-shock protein) PMID: 28592492
    10. KRAS-mutant colorectal carcinoma shows intrinsic radioresistance along with rapid upregulation of hnRNP K in response to ionizing radiation that can effectively be targeted by MEK inhibition. PMID: 27793696
    11. Nujiangexathone A, a novel compound from Garcinia nujiangensis, down-regulates hnRNPK levels in cervical tumor cells, inducing cell cycle arrest. PMID: 27424288
    12. Our study also suggests that loss of function variants in HNRNPK should be considered as a molecular basis for patients with Kabuki-like syndrome PMID: 26954065
    13. these findings support a critical role for hnRNP K in the regulation of autophagy in drug-resistant leukemia cells, and this RNP is therefore a potential target of clinical drug-resistance treatment. PMID: 27155326
    14. hnRNP K is a multifunctional protein that can regulate both oncogenic and tumor suppressive pathways through a plethora of chromatin-, DNA-, RNA-, and protein-mediated activates, suggesting its aberrant expression may have broad-reaching cellular impacts. (Review) PMID: 27049467
    15. These results establish the role of hnRNP K and PCPB1 in the translational control of morphine-induced MOR expression in human neuroblastoma (NMB) cells as well as cells stably expressing MOR (NMB1). PMID: 27292014
    16. HnRNP K is a promising tissue biomarker for diagnosing gastric cancer. PMID: 27278897
    17. CASC11 can target heterogeneous ribonucleoprotein K (hnRNP-K) to activate WNT/beta-catenin signaling in colorectal cancer cells to promote tumor growth and metastasis. PMID: 27012187
    18. Data show that heterogeneous nuclear ribonucleoprotein K (hnRNPK) stabilized of cellular FLICE-inhibitory protein (c-FLIP) protein through inhibition of glycogen synthase kinase 3 beta (GSK3beta) Ser9 phosphorylation during the TNF-related aoptosis-inducing ligand (TRAIL)-induced apoptosis. PMID: 26972480
    19. HhnRNP-K-mediated regulation of NMHC IIA mRNA translation contributes to the control of enucleation in erythropoiesis. PMID: 26823606
    20. hnRNP K binds miR-122, a mature liver-specific microRNA required for Hepatitis C virus replication. PMID: 26330540
    21. The s identified two new human proteins that interact with Ehrlichia chaffeensis EtpE-C: CD147 and heterogeneous nuclear ribonucleoprotein K (hnRNP-K). PMID: 26530384
    22. Data show that proto-oncogene protein c-myc is upregulated by sumoylated heterogeneous nuclear ribonucleoprotein K (hnRNP K) at the translational level in Burkitt's lymphoma cells. PMID: 26317903
    23. hnRNP K may be a key molecule involved in cell motility in RCC cells PMID: 26713736
    24. It describes the identification of heterogeneous nuclear ribonucleoprotein K (hnRNPK) as one of the composite element binding factors(CEBF) that acts as transactivator of PXR promoter. PMID: 26586566
    25. RTVP-1 regulates glioma cell spreading, migration and invasion and that these effects are mediated via interaction with N-WASP and by interfering with the inhibitory effect of hnRNPK on the function of this protein. PMID: 26305187
    26. we investigate the role of hnRNP K in the radioresistance of malignant melanoma cells PMID: 26136337
    27. These results indicate that dengue virus type 2 and Junin virus induce hnRNP K cytoplasmic translocation to favor viral multiplication. PMID: 25865411
    28. Data implicate hnRNPK in the development of hematological disorders and suggest hnRNPK acts as a tumor suppressor. PMID: 26412324
    29. Inhibition of CDK2 phosphorylation blocked phosphorylation of hnRNP K, preventing its incorporation into stress granules (SGs). Due to interaction between hnRNP K with TDP-43, the loss of hnRNP K from SGs prevented accumulation of TDP-43. PMID: 25410660
    30. HNRNPK might determine efficiency of Hepatitis C virus particle production by limiting the availability of viral RNA for incorporation into virions. PMID: 25569684
    31. Data indicate that twenty proteins were identified as binding partners of the primary activating element in the heterogeneous nuclear ribonucleoprotein K (hnRNP K) promoter. PMID: 25497182
    32. hnRNP K interacts with EV71 5' UTR, which is required for efficient synthesis of viral RNA.[review] PMID: 26164948
    33. hnRNPK is potentially implicated in the radiogenic response of HNSCC. PMID: 25281771
    34. hnRNP K plays an important role in the mitotic process in colon cancer cells.hnRNP K upregulates NUF2 and promotes the tumorigenicity of colon cancer cells. PMID: 25701787
    35. These findings functionally integrate K17, hnRNP K, and gene expression along with RSK and CXCR3 signaling in a keratinocyte-autonomous axis and provide a potential basis for their implication in tumorigenesis PMID: 25713416
    36. HnRNP K can induce MMP12 expression and enzyme activity through activating MMP12 promoter, which promotes cell migration and invasion in nasopharyngeal carcinoma cells. PMID: 24885469
    37. Results indicate that the interaction between the AR and hnRNP K has an important role in the progression of prostate cancer. PMID: 24626777
    38. hnRNP K and PU.1 act synergistically during granulocytic differentiation, hnRNP K seems to have a negative effect on PU.1 activity during monocytic maturation PMID: 25005557
    39. CNBP overexpression caused increase of cell death and suppression of cell metastasis through its induction of G-quadruplex formation in the promoter of hnRNP K resulting in hnRNP K down-regulation PMID: 24594223
    40. Data show that SET accumulation up-regulated hnRNPK mRNA and total/phosphorylated protein, promoted hnRNPK nuclear location, and reduced Bcl-x mRNA levels. PMID: 24508256
    41. NS1-BP-hnRNPK complex is a key mediator of influenza A virus gene expression. PMID: 23825951
    42. These studies demonstrate hnRNP K to be a multifunctional protein that supports vesicular stomatitis virus infection via its role(s) in suppressing apoptosis of infected cells. PMID: 23843646
    43. hnRNPK may play a role in recruitment of XRN2 to gene loci thus regulating coupling 3'-end pre-mRNA processing to transcription termination. PMID: 23857582
    44. heterogeneous nuclear ribonucleoprotein K (hnRNPK), a protein known to integrate multiple signal transduction pathways with gene expression, as a SERT distal polyadenylation element binding protein PMID: 23798440
    45. Prolonged downregulation of hnRNP K using small interfering RNA significantly decreased cell viability and increased apoptosis in HCC cell lines in a p53-independent manner. PMID: 23455382
    46. Caspase-3 cleaves hnRNP K in erythroid differentiation. PMID: 23519117
    47. HnRNP K controlled expression of IE2 protein of Human cytomegalovirus during viral replication. PMID: 23099853
    48. hnRNP-K regulates extracellular matrix, cell motility, and angiogenesis pathways. Involvement of the selected genes (Cck, Mmp-3, Ptgs2, and Ctgf) and pathways was validated by gene-specific expression analysis PMID: 23564449
    49. ATM-dependent phosphorylation of heterogeneous nuclear ribonucleoprotein K promotes p53 transcriptional activation in response to DNA damage. PMID: 23343766
    50. SUMO modification plays a crucial role in the control of hnRNP-K's function as a p53 co-activator in response to DNA damage by UV PMID: 23092970

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  • 相关疾病：
    Au-Kline syndrome (AUKS)
  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleoplasm. Cell projection, podosome. Note=Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).
  • 数据库链接：

    HGNC: 5044

    OMIM: 600712

    KEGG: hsa:3190

    STRING: 9606.ENSP00000365439

    UniGene: Hs.522257