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  4. FYN Recombinant Monoclonal Antibody

FYN Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA699767A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA699767A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA699767A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    FYN is a non-receptor or cytoplasmatic tyrosine kinase (TK) belonging to the Src family kinases (SFKs) involved in multiple transduction pathways in the central nervous system (CNS) including synaptic transmission, myelination, axon guidance, and oligodendrocyte formation. FYN is thought to function at synaptic sites and act as a key regulator in synaptic transmission and plasticity. When disrupted by these pathways, FYN can contribute to the development of severe brain pathologies, such as Alzheimer’s disease (AD) and multiple sclerosis (MS). FYN also participates in growth factor and cytokine receptor signaling, ion channel function, platelet activation, fertilization, entry into mitosis, and other additional biological functions.

    The production of this recombinant FYN antibody started with identifying and cloning the genes for antibody expression. After the FYN antibody was cloned into an expression plasmid, the plasmid could be introduced into the mammalian cell to produce the target recombinant antibody. This recombinant FYN antibody has been validated in ELISA, IHC.

  • Uniprot No.：
    P06241
  • 基因名：
  • 别名：
    Tyrosine-protein kinase Fyn (EC 2.7.10.2) (Proto-oncogene Syn) (Proto-oncogene c-Fyn) (Src-like kinase) (SLK) (p59-Fyn), FYN
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Fyn
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1G3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling.
  • 基因功能参考文献：
    1. SPHK2 is highly expressed in the kidney interstitium of patients with renal fibrosis and highly correlates with disease progression. SPHK2 phosphorylates Fyn to activate downstream STAT3 and AKT, thereby promoting extracellular matrix synthesis, kidney fibroblast activation, and renal fibrosis. PMID: 30251698
    2. Fyn and Lyn as important factors that promote Plasmacytoid dendritic cell responses. PMID: 28368000
    3. Data suggest that Fyn tyrosine kinase (Fyn)-dependent phosphorylation at two critical tyrosines is a key feature of vertebrate plexin A1 (PlxnA1) and plexin A2 (PlxnA2) signal transduction. PMID: 29091353
    4. Nav1.7 is a substrate for Fyn kinase. PMID: 29790812
    5. Fyn binds to mGluR1a at a consensus binding motif located in the intracellular C-terminus (CT) of mGluR1a in vitro. Active Fyn phosphorylates mGluR1a at a conserved tyrosine residue in the CT region. In cerebellar neurons and transfected HEK293T cells, Fyn-mediated tyrosine phosphorylation of mGluR1a is constitutively active and facilitates surface expression of mGluR1a and potentiates mGluR1a postreceptor signaling. PMID: 28948209
    6. High FYN expression is associated with pancreatic cancer metastasis. PMID: 28560430
    7. Study was the first to demonstrate critical positive regulation of thyroid tumorigenesis by FYN, which could be a potential target gene for thyroid carcinoma treatment. PMID: 29140740
    8. Fyn-dependent phosphorylation of SHP-1 serine 591 inactivates the phosphatase, enabling activatory immunoreceptor signaling. PMID: 28811476
    9. upregulated in fibrotic kidneys PMID: 27616741
    10. Study identified the binding site between tau and fyn-SH3 may facilitate the development of compounds that can inhibit tau-fyn interactions, which presents an alternative therapeutic strategy for Alzheimer's disease; and provide evidence that a physiological correlation between phosphorylated tau at S202, S262, and S396/404 and fyn is not present in Alzheimer's disease brain. PMID: 27193083
    11. FYN expression is regulated according to AD status and regulatory region haplotype, and genetic variants may be instrumental in the development of neurofibrillary tangles in AD and other tauopathies. PMID: 28033507
    12. a substantial fraction of unligated CD36 exists in nanoclusters, which not only promote TSP-1 binding but are also enriched with the downstream effector Fyn. PMID: 27694211
    13. Upon SMAD4 deletion, we detected high expression levels of FYN in vessel endothelial cells, suggesting the mechanism of the ovarian tumor cells cross the endothelial barrier and transform to an invasive phenotype PMID: 28393199
    14. Study reveal that binding the phosphorylated tail of Fyn perturbs a residue cluster near the linker connecting the SH2 and SH3 domains of Fyn, which is known to be relevant in the regulation of the activity of Fyn. PMID: 27692963
    15. The data suggest that miR-106b inhibits Amyloid-beta (1-42)-induced tau phosphorylation at Tyrosine 18 by targeting Fyn. PMID: 27520374
    16. FYN was transcriptionally regulated by FOXO1. PMID: 27349276
    17. Results found that GluN2B subunit-containing NMDARs were dominant in induced pluripotent stem cell-derived neurons and that tyrosine-protein kinase Fyn potentiated the function of GluN2B subunit-containing NMDARs. PMID: 27040756
    18. These results indicate that the microenvironment and growth patterns in an multicellular spheroid are complex and require MAPK and FYN kinase PMID: 27466485
    19. this study shows that p59(fyn), which is essential for activation of T cells through the T-cell receptor, is also critical for signal transduction through Toll-like receptors in T cells PMID: 26888964
    20. Fyn expression fluctuated with the progress of normal pregnancy and was elevated in patients with recurrent spontaneous abortion PMID: 26892111
    21. Neuroendocrine differentiation in prostate cancer cells and visceral metastasis, are least in part, regulated by FYN kinase. PMID: 26624980
    22. Fyn facilitates mitotic spindle formation through the increase in microtubule polymerization, resulting in the acceleration of M-phase progression. PMID: 26365631
    23. Results show that three CpG loci within FYN were hypermethylated in obese individuals, while obesity was associated with lower methylation of CpG loci within PIWIL4 and TAOK3. PMID: 26646899
    24. Data provide structural insight into the dimerization of Fyn SH2 both in solution and in crystalline conditions, providing novel crystal structures of both the dimer and peptide-bound structures of Fyn SH2. PMID: 26384592
    25. Mutation of Fyn phosphorylation sites on PIKE-A, depletion of Fyn, or pharmacological inhibition of Fyn blunts the association between PIKE-A and AMPK, resulting in loss of its inhibitory effect on AMPK. PMID: 26001218
    26. Results show that Fyn differentially modulates Nav1.5 channel splice variants. It phosphorylates Nav1.5 variants Q-del and Q-pre resulting in there hyperpolarizing and depolarizing shift. Fyn's activity is abolished in the presence of both variants. PMID: 26382759
    27. In this study, it is shown that the alternatively spliced FynT isoform is specifically up-regulated in the AD neocortex, with no change in FynB isoform. PMID: 26561212
    28. our results provide no evidence that the Fyn -93A>G SNP contributes to the susceptibility to acute liver transplant rejection in a Caucasian population. PMID: 26407913
    29. Data suggest both KLF5 (Kruppel like factor 5) and FYN are important in regulation of migration in bladder cancer cells; KLF5 up-regulates cell migration, lamellipodia formation, FYN expression, and phosphorylation of FAK (focal adhesion kinase). PMID: 26786295
    30. Our results establish that Fyn can arrest SW-induced apoptosis via the activity of Akt and its effective phosphorylation in 293T cells. PMID: 26125726
    31. Fyn inhibition may be an effective therapeutic approach in treating cSCC. PMID: 24976598
    32. the main finding is that FYN polymorphisms were respon- sible for the variance in intermediate defense style(undoing)and mature defensestyle(suppression). PMID: 25130779
    33. Results indicate that FYN has an important role in tamoxifen resistance, and its subcellular localization in breast tumor cells may be an important novel biomarker of response to endocrine therapy in breast cancer. PMID: 24882577
    34. it is not just inactivation of Fyn that promotes multicellular spheroid formation but this must be coupled with the full length beta6 integrin. PMID: 25503120
    35. these results demonstrate a Fyn kinase-dependent mechanism through which IFNgamma regulates E-cadherin stability and suggest a novel mechanism of disruption of epithelial cell contact, which could contribute to perturbed epithelial barrier function. PMID: 22715382
    36. The SNPs in the selected regions of the Fyn gene are unlikely to confer the susceptibility of sAD in the Chinese Han population PMID: 24852829
    37. Fyn expression in mdMSCs contributes to basal cytoskeletal architecture and, when associated with FAs, functions as a proximal mechanical effector for environmental signals that influence MSC lineage allocation. PMID: 23836527
    38. These results provided suggestive evidence that the FYN gene contributes toward the variance in human coping styles. PMID: 23851594
    39. monomeric IgE, in the absence of antigen, induces VEGF production in MC and in vivo contributes to melanoma tumor growth through a Fyn kinase-dependent mechanism. PMID: 23915951
    40. positively regulates IFN-lambda1 genes during viral infection PMID: 23405030
    41. PrP(C) down-regulated tau via the Fyn pathway and the effect can be regulated by Abeta oligomers. PMID: 23805846
    42. Mutations in FYN implicate SRC signaling, impaired DNA damage response, and escape from immune surveillance mechanisms in the pathogenesis of peripheral T cell lymphomas. PMID: 24413734
    43. MicroRNA-125a-3p reduces cell proliferation and migration by targeting Fyn. PMID: 23606749
    44. These data demonstrate a mechanism whereby Fyn and Lyn, redundantly mediate anticryptococcal killing by inducing the polarization of perforin-containing granules to the NK cell-cryptococcal synapse. PMID: 23918783
    45. no association between FYN polymorphisms and schizophrenia risk or age at schizophrenia onset, was found. PMID: 23250004
    46. Expression of HPV type 16 E7 resulted in increase in Src and Yes proteins level, but did not alter the level of Fyn. PMID: 23497302
    47. Highlighted is recent evidence that FYN kinase mediates signal transduction downstream of the PrP(C)-ABETA oligomer complex in Alzheimer disease. [review] PMID: 22987042
    48. Fyn regulates the activity of the adipogenic transcription factor signal transducer and activator of transcription 5a (STAT5a) through enhancing its interaction with the GTPase phosphoinositide 3-kinase enhancer A (PIKE-A). PMID: 23438599
    49. Nck may facilitate dynamic signaling events at the slit diaphragm by promoting Fyn-dependent phosphorylation of nephrin. PMID: 23188823
    50. The results presented from this in silico study will open up new prospect for genetic analysis of FYN gene and their correlation with clinical data will be very useful in understanding the genetics of Alzheimer's disease. PMID: 22843238

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cell membrane. Note=Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, SRC subfamily
  • 组织特异性：
    Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
  • 数据库链接：

    HGNC: 4037

    OMIM: 137025

    KEGG: hsa:2534

    STRING: 9606.ENSP00000346671

    UniGene: Hs.390567