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  4. FLI1 Recombinant Monoclonal Antibody

FLI1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA207775A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, U-251 whole cell lysate, Hela whole cell lysate
      All lanes: FLI1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 51, 44, 48, 30 kDa
      Observed band size: 51 kDa
  • 其他：

产品详情

  • 产品描述：

    FLI1, a member of the Ets family, is expressed in fibroblasts, endothelial cells, and immune cells. FLI1 gene is involved in the development, proliferation, activation, migration, and other processes of immune cells. FLI1 can also affect the function of immune cells by regulating cytokines and chemokines. Emerging evidence has shown that FLI1 is implicated in the etiology of several autoimmune diseases, including systemic sclerosis (SSc) and systemic lupus erythematosus (SLE). Abnormal expression of the FLI1 transcription factor following genetic mutations has been considered a seminal event in the initiation of certain types of malignant transformation and tumor progression.

    The production of this recombinant FLI1 antibody started with immunization. And then the workflow included B cell harvest and enrichment; import single B cell; assays to identify the specificity, affinity & functionality of the cell; export the single B cell; cDNA synthesis and sequencing; express the FLI1 antibody in mammalian cells. The target FLI1 antibody was validated in ELISA, WB.

  • Uniprot No.：
    Q01543
  • 基因名：
    FLI1
  • 别名：
    Friend leukemia integration 1 transcription factor (Proto-oncogene Fli-1) (Transcription factor ERGB), FLI1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human FLI1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4E5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • 基因功能参考文献：
    1. These results indicate that Fli1 deficiency promotes migration, proliferation and cell survival, while abating tube formation of endothelial cells, suggesting that Fli1 deficiency is potentially attributable to the development of both proliferative obliterative vasculopathy (occlusion of arterioles and small arteries) and destructive vasculopathy (loss of small vessels) characteristic of SSc vasculopathy. PMID: 28370536
    2. Fli1 functioned as an oncogene in HCC carcinogenesis and it exerted its promoting metastatic effect primarily by modulating the matrix metalloproteinase (MMP)2 signaling pathway. PMID: 29138848
    3. Decreased serum LIF levels may be associated with vasculopathy in systemic sclerosis (SSc) and that Fli1 deficiency may contribute to the inhibition of LIF-dependent biological effects on SSc endothelial cells by suppressing the expression of LIF, LIF receptor, and gp130. PMID: 29038846
    4. The mouse (Fli1) and human Fli1 genes are similarly regulated by Ets factors in T cells. PMID: 19829305
    5. In contrast, expression of Spi1/PU.1 in a Fli1 producing erythroleukemia cell line in which fli1 is activated, resulted in increased proliferation through activation of growth promoting proteins MAPK, AKT, cMYC and JAK2 PMID: 28586009
    6. CXCL6 expression is upregulated by Fli1 deficiency in fibroblasts and endothelial cells, potentially contributing to the development of fibrosis and vasculopathy in the skin, lung, and heart of systemic sclerosis. PMID: 28507181
    7. This is the first report presenting CD13 and FLI1 as important mediators of resistance to BRAF inhibition with potential as drug targets in BRAF inhibitors refractory melanoma. PMID: 29048432
    8. This study uncovers FLI1 as an important driving factor that promotes tumor growth in SCLC through the miR-17-92 pathway. FLI1 may serve as an attractive target for therapeutic intervention of SCLC. PMID: 28410216
    9. identification of two novel FLI1 variants (c.1010G>A and c.1033A>G) responsible for macrothrombocytopenia; study provides new insights into the phenotype, pathophysiology and diagnosis of FLI1 variant-associated thrombocytopenia PMID: 28255014
    10. miR-145 acts as a tumor suppressor by directly reducing expression of FLI-1, and the miR-145/FLI-1 pathway is important for tumor progression in osteosarcoma PMID: 27304058
    11. this paper provides a mechanistic insight into the regulation of angiogenesis by TLRs and confirm a central role of Fli1 in regulating vascular homeostasis. PMID: 29141862
    12. In American cutaneous leishmaniasis caused by Leishmania braziliensis infection, MMP1 is regulated by factors other than FLI1, and the influence of IL-6 on MMP1 was independent of its effect on FLI1. PMID: 28119029
    13. epithelial Fli1 deficiency might be involved in the systemic autoimmunity and selective organ fibrosis in systemic sclerosis (SSc). PMID: 28232470
    14. study shows that FLI-1 is expressed variably in different subtypes of NSCLC, and its expression is related to clinicopathologic parameters and poorer prognosis PMID: 26317314
    15. FLI1 and MMP9 position differently in prostate cancer than in normal tissue and prostate hyperplasia, whereas MMP2 is repositioned in both prostate cancer and hyperplasia. PMID: 26564800
    16. FLI1 promoter hypermethylation is associated with Colorectal Cancer. PMID: 25472652
    17. Concurrent exogenous expression of three transcription factors, GATA1, FLI1 and TAL1, enables large-scale production of megakaryocytes from human pluripotent stem cells. PMID: 27052461
    18. This study for the first time identifies FLI1 as a clinically and functionally important target gene of metastasis, providing a rationale for developing FLI1 inhibitors in the treatment of breast cancer. PMID: 26156017
    19. SLFN11 has a role as a transcriptional target of EWS-FLI1 and is a determinant of drug response in Ewing sarcoma PMID: 25779942
    20. Fli1 is epigenetically suppressed and is a potential predisposing factor in the pathogenesis of systemic sclerosis. (Review) PMID: 26055516
    21. erythrocyte lineage enforces exclusivity through upregulation of EKLF and its lineage-specific cytokine receptor (EpoR) while inhibiting both FLI-1 and the receptor TpoR (also known as MPL) for the opposing megakaryocyte lineage PMID: 26159733
    22. Mutation in FLI1 is associated with Paris-Trousseau thrombocytopenia. PMID: 26316623
    23. Fli-1 expression gradually increases in parallel with disease progression. PMID: 26305602
    24. Simultaneous downregulation of KLF5 and Fli1 is a key feature underlying systemic sclerosis. PMID: 25504335
    25. endothelial CCN1 downregulation at least partially due to Fli1 deficiency may contribute to the development of digital ulcers in systemic sclerosis patients PMID: 25421497
    26. bosentan...increased DNA binding of Fli1 and the suppression of type I collagen expression in systemic sclerosis fibroblasts PMID: 24708674
    27. The similarity among the phenotypes of EWS/FLI1- and EWS siRNA-transfected HeLa cells points to the inhibition of EWS as the key mechanism for the induction of midzone defects. PMID: 25483190
    28. FLI1 levels are reduced in human breast cancer samples and cell lines.FLI1 expression is correlated with breast cancer cellular growth, migration, and invasion and altered gene expression. PMID: 25379017
    29. p16 and possibly Fli-1 may have utility in the assignment of growth phase for cutaneous melanomas. PMID: 25062258
    30. Results show that overexpression is intimately related to malignant phenotypes and poor clinical outcome of epithelial ovarian cancer. PMID: 24923303
    31. Fli1 bound to the CXCL5 promoter and its gene silencing significantly suppressed the CXCL5 mRNA expression in human dermal microvascular endothelial cells PMID: 24292093
    32. Report FLI1 expression in epithelioid sarcomas. PMID: 24072183
    33. Fli1 and CTGF are important mediators of the fibrogenic actions of adenosine PMID: 23663495
    34. these results indicate that under quiescent conditions Fli1 recruits HDAC1/p300 to the COL1A2 promoter and suppresses the expression of the COL1A2 gene by chromatin remodeling through histone deacetylation. PMID: 24058639
    35. these results fill a gap in the literature: the confirmation that MCC is devoid of the EWS/FLI-1 rearrangement. PMID: 23165331
    36. Overexpression of the ETS-related transcription factor ETV1 can initiate neoplastic transformation of the prostate. PMID: 23774214
    37. alterations in FLI1 and RUNX1 may be common in patients with platelet dense granule secretion defects and mild thrombocytopenia. PMID: 24100448
    38. a novel function for Fli-1 in T cell development and leukaemogenesis PMID: 23667468
    39. Podoplanin and Fli-1 immunohistochemistry may be useful in distinguishing atypical fibroxanthoma from angiosarcoma. PMID: 23518636
    40. Ciprofloxacin has antifibrotic actions in Systemic sclerosis dermal and lung fibroblasts via the downregulation of Dnmt1, the upregulation of Fli1. PMID: 23041765
    41. In 5q- syndrome, but not Diamond-Blackfan anemia, blood & bone marrow mononuclear cells had high Fli1 mRNA levels. This protects megakaryocytic cells from ribosomal stress & permits effective though dysplastic megakaryopoiesis. PMID: 22965552
    42. Our findings of SNP array adn phenotype correclation do not support the traditional view that FLI1 deletion is the cause of thrombocytopenia in Jacobsen syndrome. PMID: 22887642
    43. We found that transcriptional activation of PRKCB was directly regulated by the chimeric fusion oncogene EWSR1-FLI1 that drivesewing sarcoma growth. PMID: 22930730
    44. FLI1 is a novel ETS transcription factor involved in gene fusions in prostate cancer and that intratumor genetic heterogeneity of ETS rearrangements can occasionally be found in index primary tumors. PMID: 22081504
    45. Data show there are no differences in the distribution of immunohistochemical reactivity for CD31, CD34, D2-40, or FLI1 between AIDS-related and non-AIDS-related Kaposi sarcoma (KS) or between nodular- and patch/plaque-stage KS. PMID: 22372906
    46. Expression of FLI-1, ELF-1, and GABP activated the PF4 promoter in HepG2 cells. PMID: 21931859
    47. we have demonstrated association between FLI1 and susceptibility to CL caused by L. braziliensis. PMID: 21633373
    48. FLI1 expression is frequently abnormal and prognostically adverse in acute myeloid leukemia. PMID: 21917756
    49. a role of Fli1 as a negative regulator of the estrogen receptor alpha gene in dermal fibroblasts. PMID: 21451544
    50. these results demonstrate that in SSc fibroblasts, c-Abl is an upstream regulator of the profibrotic PKCdelta/phospho-Fli-1 pathway, via induction of PKCdelta nuclear localization. PMID: 21321929

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  • 相关疾病：
    Ewing sarcoma (ES); Bleeding disorder, platelet-type 21 (BDPLT21)
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ETS family
  • 数据库链接：

    HGNC: 3749

    OMIM: 193067

    KEGG: hsa:2313

    STRING: 9606.ENSP00000399985

    UniGene: Hs.504281