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  4. EIF4A1 Recombinant Monoclonal Antibody

EIF4A1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA190088A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate, RAW264.7 whole cell lysate
      All lanes: eIF4A1 Antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 40, 47 kDa
      Observed band size: 50 kDa
    • Immunofluorescence staining of Hela Cells with CSB-RA190088A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    In cap-dependent translation, EIF4A1 is an mRNA helicase that is involved in the unwinding of the secondary structure, such as the stem-loops, in the 5′-UTR of the mRNA. This facilitates ribosomal scanning and translation of the oncogenic mRNAs. EIF4A1 has a regulatory role in translating many oncoproteins that have vital roles in several steps of metastases. It also plays an important role in malignant transformation and progression. Recent evidence has shown that eIF4A1 is dysregulated in gastric cancer (GC), hepatocellular carcinoma, ovarian cancer, and others.

    This recombinant EIF4A1 antibody was developed with the Single B cell platform. The main process included identification and isolation of single B cells; amplification and cloning of EIF4A1 antibody gene; expression, screening, and identification of antibody specificity.  And this EIF4A1 antibody has been validated in ELISA, WB, IF.

  • Uniprot No.：
    P60842
  • 基因名：
    EIF4A1
  • 别名：
    Eukaryotic initiation factor 4A-I (eIF-4A-I) (eIF4A-I) (EC 3.6.4.13) (ATP-dependent RNA helicase eIF4A-1), EIF4A1, DDX2A EIF4A
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human eIF4A1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    7C3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
  • 基因功能参考文献：
    1. Star-PAP-specific polyadenylation sites usage regulates the expression of the eukaryotic translation initiation factor EIF4A1, the tumor suppressor gene PTEN and the long non-coding RNA NEAT1. PMID: 28911096
    2. our data demonstrate that the common effects of eIF4A1 and eIF4E on translation are mediated by the coding region and 3'UTR PMID: 27879264
    3. our data identify the eIF4F complex as an important upstream regulator of TORC1, which acts via TSC2 to inactivate TORC1 upon withdrawal of amino acids PMID: 26988032
    4. Our results demonstrate that miR-133a plays a pivotal role in colorectal cancer by inhibiting cell proliferation, invasion, and migration by targeting oncogenic eukaryotic translation initiation factor 4A1, which acts as a tumor suppressor and may provide a new potential therapeutic target in colorectal cancer PMID: 28466778
    5. miR-1284 can function as a new regulator to reduce gastric cancer multidrug resistant cells by targeting EIF4A1. PMID: 26936591
    6. Studies indicate a developing focus on targeting eukaryotic initiation factor 4A eIF4A1 and eIF4A2 as cancer therapy. PMID: 26614665
    7. Immunohistochemical analysis was performed on over 3000 breast tumors to investigate the relationship among expression of eIF4A1, the helicase-modulating proteins eIF4B, eIF4E and PDCD4, and clinical outcome. PMID: 25611378
    8. Collectively, these data suggested that a potential role of NS4A in antagonizing host antiviral defense is by recruiting eIF4AI and escaping the translation inhibition mediated by PKR. PMID: 25866185
    9. eIF4A functions as an adenosine triphosphate-dependent processive helicase when complexed with two accessory proteins, eIF4G and eIF4B. PMID: 26113725
    10. demonstrate that DAP5 associates with eIF2beta and eIF4AI to stimulate IRES-dependent translation of cellular mRNAs PMID: 25779044
    11. Studies indicate that eukaryotic initiation factor 4AI (EIF4AI) undergoes large conformational transitions, while unwinding RNA hairpins. PMID: 24909782
    12. Data indicate that caspase recruitment domain family, member 11 protein CARD11 has complex 5'UTRs and is sensitive to eIF4A RNA helicase inhibition. PMID: 25320244
    13. Increased Expression of EIF4A1 is associated with poor response to Brachytherapy in Cervical Cancer. PMID: 24844222
    14. report of an eIF4A RNA helicase-dependent mechanism of translational control that contributes to oncogenesis and underlies the anticancer effects of silvestrol and related compounds PMID: 25079319
    15. The E186 residue was found to be of significance for RNA-dependent ATPase activity for eIF4AI alone and in the presence of eIF4AI-binding domains of eIF4GI, binding between eIF4AI and eIF4GI domains were also significantly influenced by mutation of E186. PMID: 24471916
    16. Findings implicate phosphorylation of eIF4G1(Ser1232) by Cdk1:cyclin B and its inhibitory effects on eIF4A helicase activity in the mitotic translation initiation shift. PMID: 24248602
    17. Eukaryotic translation initiation factor 4A1 (eIF4A1) is a direct target of miR-US25-2-3p. PMID: 23747307
    18. Regulation of MUC1-C expression is mediated by the PI3K/AKT pathway and the eIF4A RNA helicase. PMID: 22689062
    19. The results indicated that eIF4AI and eIF4AII expression are linked and that the two protein isoforms exhibit functional differences. PMID: 22589333
    20. Duplex unwinding and ATPase activities of the DEAD-box helicase eIF4A are coupled by eIF4G and eIF4B. PMID: 21840318
    21. study found Burkholderia pseudomallei BPSL1549 acted as a potent cytotoxin against eukaryotic cells; it promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation PMID: 22076380
    22. analysis of the tandem MA-3 region of Pdcd4 protein and characterization of its interactions with eIF4A and eIF4G PMID: 21454508
    23. Studies indicate that eIF4A (DDX2), together with its accessory proteins eIF4B and eIF4H, is thought to act as a helicase that unwinds secondary structures in the mRNA 5' UTR. PMID: 21427765
    24. EIF4A and eIF4G proteins are not responsible for the selective translation of viral mRNAs and the translational shut-off of cellular protein synthesis. PMID: 21377182
    25. Inhibition of eIF4A reduced the synthesis of APP and tau. PMID: 20927385
    26. These findings identify PKP1 as a regulator of translation and proliferation via modulation of eIF4A1 activity. PMID: 20156963
    27. Comparative characterization of two DEAD-box RNA helicases in superfamily II: human translation-initiation factor 4A and hepatitis C virus non-structural protein 3 (NS3) helicase. PMID: 11903057
    28. Results indicate that not only binding to eIF4A but also prevention of eIF4A binding to the MA-3 domain of eIF4Gc contributes to the mechanism by which Pdcd4 inhibits translation. PMID: 15082783
    29. analysis of cells of eIF4GIf molecules lacking either the PABP-binding site, the eIF3-binding site, the middle domain eIF4A-binding site, or the C-terminal segment that includes the second eIF4A-binding site PMID: 17130132
    30. Results report that 15d-PGJ2 blocks translation through inactivation of translational initiation factor eIF4A. PMID: 18034160
    31. the PDCD4 MA3 domains compete with the eIF4G MA3 domain and RNA for eIF4A binding. PDCD4 inhibits translation initiation by displacing eIF4G and RNA from eIF4A. PMID: 18296639
    32. The interaction of eIF4AI with two accessory factors, eIF4B and eIF4H, was studied. PMID: 18719248
    33. Study reports the topology of the eIF4A/4G/4H helicase complex, which is built from multiple experimentally observed domain-domain contacts. PMID: 19203580

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  • 蛋白家族：
    DEAD box helicase family, eIF4A subfamily
  • 数据库链接：

    HGNC: 3282

    OMIM: 602641

    KEGG: hsa:1973

    STRING: 9606.ENSP00000293831

    UniGene: Hs.129673