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  4. DOT1L Recombinant Monoclonal Antibody

DOT1L Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA238318A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate
      All lanes: DOT1L antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 165 kDa
      Observed band size: 165 kDa
    • Immunofluorescence staining of Hela cell with CSB-RA238318A0HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • 产品描述：

    The recombinant DOT1L antibody is a monoclonal antibody molecule expressed by using recombinant DNA and protein engineering technology to clone the genes encoding the DOT1L antibody into a plasma vector and then by transfecting the vector clone into the appropriate recipient mammalian cells for production. It was purified using affinity-chromatography. And it shows reactivity with DOT1L protein from Human. This recombinant DOT1L antibody can be used in the ELISA, WB.

     

    DOT1L is a specific methyltransferase responsible for histone H3K79 methylation. DOT1L-dependent H3K79 methylation participates in several cellular processes, including transcription elongation by RNA polymerase II, the DNA damage response, and cell cycle checkpoint activation. Mammalian DOT1L regulates transcription, embryonic development, erythropoiesis, differentiation, and proliferation of normal cells. DOT1L gene deletions and somatic mutations have been reported in melanoma, colorectal cancer, and ovarian cancer, among other solid tumors. DOT1L is also involved in the pathogenesis of AMLs with mixed-lineage leukemia (MLL) gene translocations.

  • Uniprot No.：
    Q8TEK3
  • 基因名：
    DOT1L
  • 别名：
    Histone-lysine N-methyltransferase, H3 lysine-79 specific (EC 2.1.1.43) (DOT1-like protein) (Histone H3-K79 methyltransferase) (H3-K79-HMTase) (Lysine N-methyltransferase 4), DOT1L, KIAA1814 KMT4
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human KMT4 / Dot1L
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3B6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.
  • 基因功能参考文献：
    1. Each C-Nap1 ring at the proximal end of the two centrioles organizes a rootletin ring and, in addition, multiple rootletin/CEP68 fibers. PMID: 29463719
    2. Gene and protein expression of DOT1L was increased in synovial tissues of both osteoarthritis and rheumatoid arthritis patients. PMID: 29234911
    3. human mammary epithelial cells reprogramming is dependent on gene silencing by the DNA methyltransferase DNMT3A and loss of histone transcriptional marks following downregulation of the methyltransferase DOT1L. PMID: 28781076
    4. this study shows that Dot1l expression predicts adverse postoperative prognosis of patients with clear-cell renal cell carcinoma PMID: 27713173
    5. facilitates DNA damage repair; plays a protective role in ultraviolet radiation-induced melanomagenesis PMID: 29343685
    6. DOT1L cooperates with transcription factor ETS-1 to stimulate the expression of VEGFR2, thereby activating ERK1/2 and AKT signaling pathways and promoting angiogenesis. PMID: 27626484
    7. findings demonstrate that DOT1L over-expression has important clinical significance in ovarian cancer and also clarify that it drives cell cycle progression through transcriptional regulation of CDK6 and CCND3 through H3K79 methylation PMID: 28114995
    8. MLL-AF4 spreading gene expression is downregulated by inhibitors of the H3K79 methyltransferase DOT1L. PMID: 28076791
    9. our results identify DOT1L as a novel cofactor in N-Myc-mediated transcriptional activation of target genes and neuroblastoma oncogenesis. Furthermore, they characterize DOT1L inhibitors as novel anticancer agents against MYCN-amplified neuroblastoma. PMID: 28209620
    10. These results reveal a cooperative transcriptional activation mechanism of AEP and DOT1L and suggest a molecular rationale for the simultaneous inhibition of the MLL fusion-AF4 complex and DOT1L for more effective treatment of MLL-rearranged leukemia. PMID: 28394257
    11. this indicates that DOT1L function, like MLL, does not completely rely on its methyltransferase activity. Nevertheless, the small molecule DOT1L inhibition is sufficient to block the proliferation of MLL fusion-induced leukemia cells of murine and human origin PMID: 26923329
    12. DOT1L may play a critical role in DNMT3A-mutant leukemia. PMID: 27335278
    13. DOT1L, via dimethylated histone H3 K79, facilitates histone H4 acetylation, which in turn regulates the binding of BRD4 to chromatin in acute lymphoblastic leukemia. PMID: 27294782
    14. study demonstrates the development of potent DOT1L inhibitors with novel scaffolds PMID: 26914852
    15. these findings strongly support the contention that histone methyltransferase, DOT1L-associated epigenetic changes induced by HA play pivotal roles in miR-10 production leading to up-regulation of RhoGTPase and survival proteins. PMID: 27002147
    16. Report drug formulation/delivery of DOT1L inhibitor pinometostat in leukemia. PMID: 26385168
    17. MLL1 and DOT1L cooperate with meningioma-1 to induce acute myeloid leukemia. PMID: 26927674
    18. These data converge on a possible mechanism for hDot1L stimulation in which histone H2B physically 'corrals' the enzyme into a productive binding orientation. PMID: 26830124
    19. expression associated with poorer survival and aggressiveness of breast cancers PMID: 26199140
    20. analysis of a functional hotspot on ubiquitin that is required for the stimulation of human Dot1 PMID: 26240340
    21. Three candidate variants were identified: p.Glu1313Lys in Insulin receptor (INSR), p.Arg81Pro in F-box protein 24 (FBXO24) and p.Pro1146Leu in DOT1-like histone H3K79 methyltransferase PMID: 25576241
    22. The graded reduction of the DOT1L interaction with MLL-AF9 results in differential loss of H3K79me2 and me3 at MLL-AF9 target genes. PMID: 25921540
    23. The PZP domain of AF10 senses unmodified H3K27 to regulate DOT1L-mediated methylation of H3K79. PMID: 26439302
    24. Targeting DOT1L and HOX gene expression in MLL-rearranged leukemia and beyond. PMID: 26118503
    25. A genome-wide association study identifies PLCL2 and AP3D1-DOT1L-SF3A2 as new susceptibility loci for myocardial infarction in Japanese PMID: 24916648
    26. DOT1L rs12982744 G to C change and variant C genotype may contribute to knee OA risk in a Chinese Han population. PMID: 25005768
    27. inhibition of DOT1L, in combination with DNA damaging chemotherapy, represents a promising approach to improving outcomes for MLL-rearranged leukemia. PMID: 24858818
    28. Studies identified the evolutionarily conserved Af9 YEATS domain as a novel acetyllysine-binding module and established a direct link between histone acetylation and DOT1L-mediated H3K79 methylation in transcription control. PMID: 25417107
    29. Results provide evidence that transformation driven by MLL fusions as well as the recurrent AML-associated NUP98-NSD1 fusion oncogene is critically dependent on the ability of AF10 to stimulate DOT1L activity. PMID: 25464900
    30. Establishing the precise function of DOT1L in normal adult hematopoiesis and understanding its mode of action will aid in our understanding of the use of DOT1L as a therapeutic target in MLL-rearranged leukemia. PMID: 24854991
    31. DOT1L Regulates IL-22 Dependent Colon Cancer Stemness via H3K79 Methylation PMID: 24816405
    32. PITX2 forms complex with histone H3 lysine 4 (H3K4) methyltransferase. PITX2 complex methylates H3K4. PMID: 24486544
    33. Functional studies show that the mapped AF9/ENL interacting site is essential for immortalization by MLL-AF9, indicating that DOT1L interaction with MLL-AF9 and its recruitment are required for transformation by MLL-AF9. PMID: 23996074
    34. In male subjects, the rs12982744 polymorphism in DOT1L is associated with hip osteoarthritis. PMID: 23505243
    35. a functional interaction between hDOT1L and RNAPII targets hDOT1L and subsequent H3K79 methylations to actively transcribed genes. PMID: 23012353
    36. Data show that histone monoubiquitylation H2Aub did not influence histone methyltransferase Dot1L activity. PMID: 22619169
    37. These data are a further step to better understand the role of Wnt-signaling during chondrogenesis and cartilage homeostasis. DOT1L may represent a therapeutic target for osteoarthritis. PMID: 22566624
    38. These findings identify a novel role for Bat3 in regulating DOT1L function, which plays a critical role in DNA damage response. PMID: 22373577
    39. down-regulation of DOT1L-mediated H3K79 methylation disturbs proliferation of human cells PMID: 22190683
    40. a novel STAT1-DOT1L interaction that is required for the regulation JAK-STAT-inducible gene expression PMID: 22002246
    41. These data point to DOT1L as a potential therapeutic target in MLL-rearranged leukemia. PMID: 21741597
    42. This permitted structure-activity studies of ubiquitylated mononucleosomes that revealed plasticity in the mechanism of hDot1L stimulation and identified surfaces of ubiquitin important for activation. PMID: 20208522
    43. study describes for the first time the components of DotCom and links the specific regulation of H3K79 trimethylation by Dot1 and its associated factors to the Wnt/Wingless signaling pathway PMID: 20203130
    44. titration of the level of ubiquitylated histone H2B within the nucleosome revealed a 1:1 stoichiometry of Dot1L activation. PMID: 19799466
    45. mistargeting of hDOT1L to Hoxa9 plays an important role in MLL-AF10-mediated leukemogenesis PMID: 15851025
    46. Altered phosphorylation of histone-H3 is associated with hepatocarcinogenesis PMID: 17094487
    47. Identification of ENL-associated proteins by mass spectrometry revealed enzymes with a known role in transcriptional elongation: pTEFb and DOT1L. PMID: 17855633
    48. suggesting a widespread mechanism for parallel or sequential recruitment of DOT1L and MLL to genes in their normal "on" state PMID: 18285465
    49. demonstration, using chemically ubiquitylated H2B, of a direct stimulation of hDot1L-mediated intranucleosomal methylation of H3 K79 PMID: 18449190
    50. the CALM-AF10 fusion protein can also greatly reduce global H3K79 methylation in both human and murine leukemic cells by disrupting the AF10-mediated association of hDOT1L with chromatin PMID: 19443658

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, DOT1 family
  • 数据库链接：

    HGNC: 24948

    OMIM: 607375

    KEGG: hsa:84444

    STRING: 9606.ENSP00000381657

    UniGene: Hs.713641