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DKK1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA576260A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 293 whole cell lysate
      All lanes: DKK1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 29 kDa
      Observed band size: 38 kDa
  • 其他：

产品详情

  • 产品描述：

    DKK1 is an inhibitor of the Wnt signaling pathway. DKK1 acts as an inducer in embryonic head formation and limb morphogenesis. Inhibition of Wnt signaling by DKK1 promotes heart muscle formation in the anterior lateral mesoderm while repressing erythropoiesis. Moreover, since overexpression of DKK1 in osteoblasts causes osteopenia, it is also regarded as a negative regulator of normal bone homeostasis. Dysregulation of DKK1 has recently emerged as a potential biomarker of cancer progression and prognosis for several types of malignancies. Some studies have proven that high expression of DKK1 can occur in a variety of cancer cell lines such as liver cancer, lung cancer, breast cancer, glioma, and cervical cancer which induce apoptosis by inhibiting cell proliferation and transformation.

    The recombinant DKK1 antibody was produced by cloning antibody genes into an expression vectors, which were subsequently introduced into mammalian cells to provide animal-free antibody production. This DKK1 antibody has been validated in ELISA, WB. It has the features of improved affinity, stability, and consistency between different batches.

  • Uniprot No.：
    O94907
  • 基因名：
  • 别名：
    Dickkopf-related protein 1 (Dickkopf-1) (Dkk-1) (hDkk-1) (SK), DKK1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human DKK1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1D12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity.
  • 基因功能参考文献：
    1. Results provide evidence that DKK-1 is involved in the progression of prostate cancer (PC). DKK-1-mediated inhibition of osteoblasts, which contributes to tumor progression and osteolytic metastases, may also play a role in the development of metastases with osteoblastic features. PMID: 30116403
    2. our findings suggest that glucocorticoid-induced expression of Dkk-1 could be a key factor in modulating the differentiation ability of MSCs used for osteonecrosis of the femur head and other stem cell therapies. PMID: 29084466
    3. MicroRNA152 inhibits cell proliferation of osteosarcoma through DKK1. PMID: 29845282
    4. this study shows that DKK1 is increased in vitiligo in lesional and peri-lesional skin of acral and non-acral lesions PMID: 29605863
    5. Dkk-1 and PTH levels in psoriatic arthritis are lower than healthy female controls, in contrast with rheumatoid arthritis, in which they are increased PMID: 28634697
    6. this study suggests that higher expression of DKK1 in the dermis induced senescence in melanocytes that may lead to hypopigmentation and play role in vitiligo pathogenesis PMID: 29442138
    7. TNF-alpha, DKK1, and OPG have roles in pathogenesis of knee osteoarthritis PMID: 28676900
    8. Serum level of DKK-1 is elevated in patients with rheumatoid arthritis (RA) compared to healthy controls, suggesting an important role of DKK-1 in the pathogenesis and treatment of RA [review]. PMID: 29665496
    9. the staining intensity of DKK1 was significantly stronger in the placentas of the severe preeclampsia group PMID: 29603045
    10. Serum OPN and DKK1 levels of hepatocellular carcinoma patients could be considered as novel biomarkers showing prognostic significance after hepatectomy based on long-term survival data PMID: 29753515
    11. These findings suggested that decreased circulating DKK-1 levels are associated with the development and severity of exudative age-related macular degeneration, and have potential to become a biomarker for exudative age-related macular degeneration. PMID: 28455497
    12. In patients with rheumatoid arthritis, higher levels of DKK1 were found in patients with lower functional status.This could be explained by more structural damage; DKK1 could be used as a biomarker of joint destruction in RA. PMID: 29282619
    13. The Dickkopf-1 (DKK1) is a secreted inhibitor of beta-catenin-dependent Wnt signalling and was originally characterized as a tumour suppressor based on the prevailing view that Wnt signalling promotes cancer pathogenesis. PMID: 28574171
    14. Collectively, our data strongly suggest that stress-related neurohormones, such as GCs, cause DP cells to secrete DKK1, which in turn inhibits hair growth. PMID: 28155238
    15. the inhibitory effects of silencing miR-217 on stem cell properties and Wnt signaling were reversed by the downregulation of DKK1 in miR-217-downregulated cells. Therefore, our results indicate that miR-217 plays a vital role in the CSC-like phenotypes of Hepatocellular carcinoma (HCC) cells and may be used as a potential therapeutic target against HCC. PMID: 28849121
    16. In our cohort of pregnant women, sclerostin and DKK1 were not associated with any adverse metabolic profile, and possibly do not play relevant roles in the pathophysiology of gestational diabetes mellitus. PMID: 28277306
    17. Serum DKK-1 levels in axial spondyloarthritis depend on disease duration. As disease duration increases, DKK-1 serum levels decrease. PMID: 27297260
    18. inhibition of the mTOR pathway increases sensitivity of melanoma cells towards temozolomide, which is associated with enhanced expression of the Wnt antagonist DKK1 PMID: 28423208
    19. Inhibition of DKK1 expression effectively decrease plaque stability by attenuating endoplasmic reticulum stress-mediated cellular apoptosis through intiating the JNK pathway and inhibiting Wnt/betacatenin and following by activation of the IRE1alpha and eif2alpha/CHOP pathways. PMID: 28703797
    20. This study demonstrated that DKK-1 may be a predictor for prognosis in pancreatic ductal adenocarcinoma patients. PMID: 28749252
    21. synovial fluid Dkk-1 levels are positively related to the severity of joint damage in knee osteoarthritis. PMID: 28451870
    22. DKK1 exerts its pro-invasion function at least in part through the beta-catenin/ MMP-7 signaling pathway, suggesting DKK1 as a potential therapeutic target for human hilar cholangiocarcinoma (HCCA). PMID: 27608843
    23. Sclerostin But Not Dickkopf-1 has roles in increasing prevalence of osteoporotic fracture and lower bone mineral density in postmenopausal Korean women PMID: 27289555
    24. High serum levels of sclerostin and Dkk-1 are associated with acute ischaemic stroke PMID: 27573735
    25. regulated by Dkk-1 secreted by Multiple Myeloma cells and is a potential mechanism contributing to the osteoblast dysfunction noted in Multiple Myeloma. PMID: 26763740
    26. DKK1 c.121G>A (p.(A41T)) variant was identified in the family with Chiari malformation type 1. Screening of DKK1 in a cohort of 65 CMI sporadic patients identified another missense variant, the c.359G>T (p.(R120L)), in two unrelated patients. PMID: 28513615
    27. LRP6 ectodomain becomes highly compact upon complexation with the Wnt antagonist Dkk1, suggesting a potential role for the ectodomain conformational change in the regulation of receptor oligomerization and signaling. PMID: 28052259
    28. Our results suggest that measurement of DKK-1 combined with DKK-1 autoantibodies is a potentially valuable tool for the early detection of ESCC. PMID: 26988995
    29. TET1 potently inhibited canonical Wnt/beta-catenin signaling by demethylating and upregulating two upstream antagonists of this pathway, SFRP2 and DKK1, which was associated with inhibition of EMT and cancer cell metastasis. PMID: 28851501
    30. This is the first study demonstrating a link between B cell depletion and skin Dkk-1 upregulation in patients with systemic sclerosis PMID: 27208972
    31. We found significantly increased levels of DKK-1 in patients with acute CO poisoning, compared to healthy controls. High levels of serum DKK-1 in patients were significantly correlated with severe neurological symptoms. PMID: 27353797
    32. DKK1 prevents lung metastasis of breast cancer cells but promotes breast-to-bone metastasis. PMID: 28892080
    33. Structure of the dual-mode Wnt regulator Kremen1 and insight into ternary complex formation with LRP6 and DKK1 have been presented. PMID: 27524201
    34. Expression of Cancer stem cells-associated DKK1 mRNA might be an unfavorable prognostic marker for patients with hepatocellular carcinoma. PMID: 28870909
    35. In chronic kidney disease, serum levels of the Wnt inhibitors DKK1 and sclerostin are unrelated, indicating different sites of origin and/ or different regulatory mechanisms. Sclerostin, as opposed to DKK1, may qualify as a biomarker of chronic kidney disease-mineral and bone and mineral disorder (CKD-MBD), particularly in dialysis patients. PMID: 28493902
    36. endogenous expression of the WNT antagonists DKK1 and FRZB is necessary for multiple steps during chondrogenesis PMID: 27733096
    37. The expression level of DKK1 in serum was increased in breast cancer patients, suggesting that serum expression level of DKK1 could be a useful biomarker in breast cancer. PMID: 28248066
    38. Results show that the expression of DKK1 is elevated in human HBV-related hepatocellular carcinoma tissues. PMID: 28419472
    39. DKK-1 and PDCD5 can be independent predictors of overall survival in patients suffering from chondrosarcoma. PMID: 27255549
    40. In patients with hepatocellular carcinoma serum DKK1 level correlated with tumor size, consequently, patients with bigger tumors tend to have higher levels of DKK1. PMID: 27161933
    41. Our results indicate that DKK1 and SFRP1 may be potentially useful biomarkers for evaluating the beneficial effects of long-term exercise on physical fitness and metabolism as well as the prognosis of patients with cancer. PMID: 28178355
    42. Dickkopf-1 and sclerostin were never correlated with each other or with bone turnover markers patients with Paget's disease of bone PMID: 28054306
    43. these findings suggest that DKK1 induces the occurrence of epithelial-mesenchymal transition and promotes migration and invasion in non-small cell lung cancer cells. Mechanically, b-catenin plays a vital role in DKK1-induced non-small cell lung cancer cell migration and invasion, and DKK1 inhibits the phosphorylation of b-catenin, resulting in the increased nuclear localization of b-catenin. PMID: 28677426
    44. Results suggest that miR-107 is likely to inhibit the occurrence and development of osteosarcoma by down-regulating Dkk-1 via the Wnt/beta-catenin signaling pathway. PMID: 28682874
    45. Recombinant Dkk1 suppresses beta-catenin target genes in myeloid-derived suppressor cells (MDSCs) from mice and humans and anti-Dkk1 loses its antitumor effects in mice lacking beta-catenin in myeloid cells or after depletion of MDSCs, demonstrating that Dkk1 directly targets MDSCs. PMID: 27045006
    46. Luciferase-fused 3' untranslated region of human Dickkopf1 activity was highly upregulated in A1CF-overexpressed MCF7 cells, but this upregulation can be inhibited by mutating conserved binding motifs of Dickkopf1 3' untranslated region. A1CF played a crucial role in cell migration and survival through affecting 3' untranslated region of Dickkopf1 to upregulate its expression in MCF7 cells. PMID: 28639893
    47. Circulating sclerostin and Dickkopf-1 levels do not change across the menstrual cycle and do not demonstrate any relationship with estradiol in premenopausal women. PMID: 27601021
    48. Results found miR-203 and DKK1 to be expressed at differential levels in cisplatin-sensitive and -insensitive lung adenocarcinoma tissues; there was also a negative correlation between them. PMID: 28350100
    49. Serum levels of OPG and DKK-1 were measured in 97 rheumatoid arthritis patients who were treated according to a treat-to-target strategy (T2T) aimed at remission (DAS28<2.6). PMID: 27911913
    50. DKK1 can promote vasculogenic mimicry formation via induction of the expression of epithelial-mesenchymal transition and cancer stem-like cell-related proteins. PMID: 27240974

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Dickkopf family
  • 组织特异性：
    Placenta.
  • 数据库链接：

    HGNC: 2891

    OMIM: 605189

    KEGG: hsa:22943

    STRING: 9606.ENSP00000363081

    UniGene: Hs.40499