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CR1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA714310A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA714310A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA714310A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    CR1, also known as CD35, is a single chain, type I transmembrane glycoprotein that belongs to the regulators of complement activation (RCA) family. It's a C3b and C4b membrane receptor found on erythrocytes, leukocytes, and podocytes. It aids in the elimination of immune complexes as well as pathogens coated in C3b and C4b. On erythrocytes, CR1 is responsible for the Knops blood group. It also acts as a cofactor for factor 1-mediated cleavage of C3b to iC3b, C3c, and C3dg, which regulates complement cascade activation by limiting the synthesis of classical and alternative route convertases and acting as a cofactor for factor 1-mediated cleavage of C3b to iC3b, C3c, and C3dg. The difference in expression of CR1 seems to correlate directly with the development of systemic lupus erythematosus (SLE) and severe forms of malaria.

    The first step in the preparation of recombinant CR1 antibody is to obtain the CR1 antibody gene. The heavy and light chain genes of the antibody were constructed into a plasma vector and then transfected into suspended mammalian cells transiently. After expression verification, cell supernatant was collected in expanded culture and purified recombinant CR1 antibody was obtained using Affinity-chromatography. This recombinant CR1 antibody has been validated for the detection of CR1 protein from Human in the ELISA, IHC.

  • Uniprot No.：
    P17927
  • 基因名：
  • 别名：
    Complement receptor type 1 (C3b/C4b receptor) (CD antigen CD35), CR1, C3BR
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CD35
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    9A9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Membrane immune adherence receptor that plays a critical role in the capture and clearance of complement-opsonized pathogens by erythrocytes and monocytes/macrophages. Mediates the binding by these cells of particles and immune complexes that have activated complement to eliminate them from the circulation. Acts also in the inhibition of spontaneous complement activation by impairing the formation and function of the alternative and classical pathway C3/C5 convertases, and by serving as a cofactor for the cleavage by factor I of C3b to iC3b, C3c and C3d,g, and of C4b to C4c and C4d. Plays also a role in immune regulation by contributing, upon ligand binding, to the generation of regulatory T cells from activated helper T cells.; (Microbial infection) Acts as a receptor for Epstein-Barr virus.
  • 基因功能参考文献：
    1. this study correlated phenotype data (CR1 density per erythrocyte (CR1/E), blood soluble CR1 (sCR1)) with genetic data (density/length polymorphisms) in AD patients and healthy controls.. PMID: 30044434
    2. CR1 rs1704660G, rs17047661G and rs6691117G variants were significantly associated with Chagas disease. PMID: 29323238
    3. Meta-analysis validated the association of late onset Alzheimer disease with CR1 (rs6656401) variants. PMID: 29504051
    4. rs6656401 CR1 is not associated with late-onset Alzheimer's disease. PMID: 28477215
    5. CR1 plays a vital role in Alzheimer's disease pathology and disease progression. PMID: 26742530
    6. the effect of CR1 rs3818361 polymorphism on Alzheimer's disease (AD) risk in Chinese cohorts is consistent with the increased risk observed in European AD cohorts. PMID: 26189835
    7. Genetic variation within the CR1 gene is associated with inflammation and the risk of incident coronary artery disease. PMID: 28033544
    8. The distribution of the CR1 alleles, genotypes and haplotypes differed significantly among population in Brazil, Vietnam, India, Republic of Congo and Ghana. PMID: 28520715
    9. CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis. PMID: 27258232
    10. This study demonstrates an up-regulation of complement regulatory proteins, CD35 and CD55 in HIV associated pre-eclamptic compared to normotensive pregnancy. PMID: 27521598
    11. Although B lymphocytes of active systemic lupus erythematosus (SLE) patients express lower level of CR1, the inhibitory capacity of this complement receptor is still maintained and its ligand-induced clustering results in significant inhibition of the main B cell functions, similar to that found in the case of healthy individuals. PMID: 27981054
    12. CyaA binding did not trigger downstream signaling of CR3 in human monocytes and CyaA-catalyzed elevation of cAMP effectively blocked CR3 signaling initiated by a natural ligand. PMID: 26650353
    13. the Putative Role of CR1 in Alzheimer's Disease PMID: 26914463
    14. Knops blood group antigen a/b genotype was associated with increased susceptibility to severe malaria and the b/b genotype was associated with reduced risk of severe malaria. PMID: 25916414
    15. The C4d/CR1 ratio is a simple and quickly determinable biomarker that enables the differentiation between infection and flare-up in febrile SLE patients at initial evaluation. PMID: 26273660
    16. This meta-analysis further supports previous findings that CR1 rs6656401 polymorphism contributes to Alzheimer's Disease susceptibility. PMID: 24878768
    17. Complement receptor type 1 (CR1/CD35) expressed on activated human CD4+ T cells contributes to generation of regulatory T cells PMID: 25742728
    18. These results provide a framework for understanding how loss of CR1 expression on podocytes may contribute to complement-mediated damage in the kidney. PMID: 26260209
    19. findings provide evidence that the A carrier (AG/GG) and the A allele of the ECR1 A3650G polymorphism may be correlated to the pathogenesis of NRDS and, hence, might be involved in the susceptibility to NRDS. PMID: 25494101
    20. THE COMBINATION OF EXPRESSION OF MARKERS CR1 AND CR2 (CD35/CD21) IN DIAGNOSTIC OF B-CELL LYMPHOPROLIFERATIVE DISEASES PMID: 26189291
    21. CR1 gene rs2274567 G/A, rs4844600 G/A, and rs2296160 C/T polymorphisms may not be involved in susceptibility to malaria in Chinese population. PMID: 25683978
    22. In addition to being a risk factor for Alzheimer's disease development, a CR1 SNP appears to be associated with higher rates of medium-term disease progression PMID: 24998857
    23. Data indicate that complement activation promote complement component 3 C3b deposition on native and acetylated LDL and binding to the complement receptor 1 (CR1). PMID: 25349208
    24. A meta-analysis demonstrated significant associations of both the CR1 rs6656401 and CR1 rs3818361 polymorphisms with late-onset Alzheimer's disease susceptibility. PMID: 24996192
    25. CR1 SNPs correlates with specific aspects of cognitive impairment in Alzheimer's disease patients. PMID: 24530172
    26. This study identified nominally significant or suggestive associations between the LOAD-risky CR1 variants and worse Logical Memory immediate recall scores in blacks. PMID: 23643458
    27. Increased CR1 expression was associated with histology classification. PMID: 23464487
    28. CR1 rs7525160 G > C polymorphism was associated with an increased risk of developing non-small cell lung cancer in Chinese population PMID: 24621201
    29. Polymorphism of the complement receptor 1 gene correlates with the hematologic response to eculizumab in patients with paroxysmal nocturnal hemoglobinuria. PMID: 24038027
    30. this is the first study to elucidate the relationship of the L-CR1 transcript with the disease activity of RA patients. PMID: 24433281
    31. Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor. PMID: 24214979
    32. Maternal coding variants in complement receptor 1 is associated with spontaneous idiopathic preterm birth. PMID: 23591632
    33. two Alzheimer's disease-associated CR1 variants, rs6656401 and rs3818361, are associated with major recurrent depression in females in a population-based cohort using individuals from the Generation Scotland: Scottish Family Health Study PMID: 22244847
    34. It did not explain (part of) the association of genome wide association top single-nucleotide polymorphisms rs3818361/rs6656401, nor of the CR1copy number variation, with Alzheimer disease in our cohort PMID: 23582656
    35. ATP release following CR1 ligation increases the mobility of the lipid fraction of RBC membranes, which in turn facilitates CR1 clustering, and thereby enhances the binding avidity of complement-opsonized particles to the RBC CR1. PMID: 24022490
    36. Data show that increased CD35, CD46, CD55 and CD59 on neutrophils and monocytes present potent markers of bacterial infection and viral infection. PMID: 23376460
    37. The CD35 antigen bounds gp350/220 and became latently infected when the fusion receptor HLA II was coexpressed. PMID: 23416052
    38. Genome-wide association studies found 3 new susceptibility loci for Alzheimer disease(AD): complement receptor 1,clusterin,and the phosphatidylinositol-binding clathrin assembly protein. These SNPS are not associated with AD in a Polish population. PMID: 23650005
    39. Our findings suggest complex mechanisms underlying the interaction of CR1, APOE, and brain amyloid pathways in Alzheimer disease PMID: 23022416
    40. Complement receptor type 1 (CR1, CD35) is a potent inhibitor of B-cell functions in rheumatoid arthritis patients. PMID: 22962438
    41. We identified rare small events overlapping CR1 and BIN1 in Alzheimer's disease and normal controls with opposite copy number variation dosage. PMID: 23202439
    42. Malaria inhibits surface expression of complement receptor 1 in monocytes/macrophages, causing decreased immune complex internalization. PMID: 23440418
    43. There are fewer mono- or dinucleated, but dramatically more numbers of tri- or polynucleated megakaryocytes in the bone marrow of transgenic Crry mice. PMID: 23390291
    44. Common CR1 variants significantly protect against severe malaria in an endemic area. PMID: 23152904
    45. In conclusion, despite there are some limitations, this meta-analysis indicates that the A allele of the CR1 SNP rs6656401 is significantly associated with LOAD susceptibility in Chinese Han populations. PMID: 22960360
    46. The clustering phenomenon of CR1 in erythrocytes is identified in electron microscopy. PMID: 22374251
    47. our results support that the CR1-S isoform explains the GWAS signals and open a novel prospect for the investigation of CR1-related disease mechanisms. PMID: 22819390
    48. Simultaneous quantitative analysis of CD64 and CD35 expression on neutrophils might be useful to distinguish between bacterial and viral infections in rheumatoid arthritis patients. PMID: 22237765
    49. CR1 (CD35) and CR3 (CD11b/CD18) act in concert for phagocytosis of opsonized Francisella tularensis by human neutrophils. PMID: 22888138
    50. CLU and CR1 were associated with more rapid cognitive decline. PICALM was associated with an earlier age at midpoint of cognitive decline. PMID: 22952074

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Receptors of complement activation (RCA) family
  • 组织特异性：
    Present on erythrocytes, a subset of T cells, mature B cells, follicular dendritic cells, monocytes and granulocytes.
  • 数据库链接：

    HGNC: 2334

    OMIM: 120620

    KEGG: hsa:1378

    STRING: 9606.ENSP00000356016

    UniGene: Hs.334019