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  4. CHEK1 Recombinant Monoclonal Antibody

CHEK1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA176809A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate
      All lanes: Chk1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 55, 44, 51 kDa
      Observed band size: 55 kDa
    • IHC image of CSB-RA176809A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela Cells with CSB-RA176809A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
  • 其他：

产品详情

  • 产品描述：

    The CHEK1 protein kinase, also called CHK1, mainly functions to coordinate the DDR and cell cycle checkpoint response. It is essential to deal with replication stress (RS) and ensure genome integrity and cell survival. Instrumental to the ATR-mediated response to RS is the downstream effector kinase CHK1, which, by controlling replication origin firing, delaying cell cycle progression, and stabilizing stalled replication forks, creates a time window to resolve DNA lesions, and ensures that cells do not enter mitosis when replication is incomplete. Additionally, CHK1 also regulates numerous other cellular functions, including DNA damage repair, gene transcription, embryo development, and somatic cell viability.

    To produce this recombinant CHEK1 antibody, we needed to get the gene sequence of the antibody.  B cell screening was used in the process. Once the sequence was obtained, it would be lead to the expression plasmids so that the CHEK1 antibody can be expressed in mammalian cells.  Moreover, this recombinant CHEK1 antibody was validated in ELISA, WB, IHC, IF.

  • Uniprot No.：
    O14757
  • 基因名：
  • 别名：
    Serine/threonine-protein kinase Chk1 (EC 2.7.11.1) (CHK1 checkpoint homolog) (Cell cycle checkpoint kinase) (Checkpoint kinase-1), CHEK1, CHK1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Chk1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    2F2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.
  • 基因功能参考文献：
    1. These results indicate that, under stressful conditions, maintained mTORC1 signaling in cancer cells promotes survival by suppressing endogenous DNA damage, and may control cell fate through the regulation of CHK1. PMID: 28484242
    2. Chk1 and 14-3-3 proteins cooperate to inactivate the transcriptional repressor functions of atypical E2F proteins. This mechanism might be of particular importance to cancer cells, since they are exposed frequently to DNA-damaging therapeutic reagents. PMID: 29363506
    3. Study provides evidence that expression of CHEK1 protein is high in breast tumors arising in Nigerian women and is associated with aggressive cancer phenotypes and is a prognostic marker. PMID: 29075961
    4. This study reports the crystal structure of the human Chk1 putative kinase-associated 1 (KA1) domain, demonstrating striking structural homology with other sequentially diverse KA1 domains. Separately purified Chk1 kinase and KA1 domains are intimately associated in solution, which results in inhibition of Chk1 kinase activity. PMID: 28972186
    5. The nuclear transcription factor Y subunit beta (NFYB)-E2F transcription factor 1 (E2F1) pathway displays a crucial role in the chemoresistance ofoxaliplatin-resistant colorectal cancer (OR-CRC) by inducing the expression and activation of checkpoint kinase 1 (CHK1), suggesting a possible therapeutic target for oxaliplatin resistance in CRC. PMID: 29203250
    6. Blocking apoptosis alone is insufficient to allow the subsequent outgrowth of primary B cells lacking CHK1 in vivo or B lymphoma lines in vitro, as these cells trigger p53- dependent cell cycle arrest in response to the accumulating DNA damage. PMID: 29167438
    7. Chk1 and Chk2 are significantly expressed in human sperm. In case of sperm DNA damage, up-regulated Chk1 expression may enhance sperm apoptosis and lead to asthenospermia, while increased Chk2 expression may inhibit spermatogenesis and result in oligospermia. PMID: 29658237
    8. CHK1 and CHK2 and their activated forms are frequently expressed in HGSC effusions, with higher expression following exposure to chemotherapy, and their expression is related to survival. PMID: 29804637
    9. expression of AURKA and CHEK1 was linked with detrimental outcome in patients. Our data describe a synthetic lethality interaction between CHEK1 and AURKA inhibitors with potential translation to the clinical setting PMID: 28847989
    10. Expression levels of phosphorylated cdc25A (p-cdc25A) and phosphorylated Chk1 (p-Chk1), belonging to the ATR pathway, were decreased by treatment with Dclk1 inhibitor LRRK2-IN-1 (LRRK), indicating Dclk1 involvement in the ATR pathway PMID: 29048622
    11. these data demonstrate that prexasertib is a specific inhibitor of CHK1 in neuroblastoma and leads to DNA damage and cell death in preclinical models of this devastating pediatric malignancy. PMID: 28270495
    12. results show that HGF was involved in regulating Chk1 phosphorylation, and further demonstrate that AKT activity was responsible for this HGF-induced Chk1 phosphorylation. PMID: 28573382
    13. Chk1 was linked to DNA damage response bypass by suppressing JNK activation following oxidative stress, promoting cell cycle progression despite DNA damage. PMID: 28751935
    14. inhibition of Chk1 can potentiate activity of nucleoside analogs in TP53-mutated B-lymphoid cells PMID: 27556692
    15. Data show that protein phosphatase-1 alpha (PP1alpha) is required to maintain checkpoint kinase 1 (CHK1) in a dephosphorylated state and for the accelerated replication fork progression in Spi1/PU.1 transcription factor-overexpressing cells. PMID: 28415748
    16. Chk1 inhibition with GDC-0425 in combination with gemcitabine was tolerated with manageable bone marrow suppression. The observed preliminary clinical activity warrants further investigation of this chemopotentiation strategy PMID: 27815358
    17. Data show that the checkpoint kinase 1/2 (Chk1/Chk2) inhibitor prexasertib (LY2606368) inhibits cell viability in B-/T-ALL cell lines. PMID: 27438145
    18. we demonstrate that CHK1 mRNA is overexpressed in two independent cohorts of medulloblastoma patient samples in comparison to normal cerebellum PMID: 27449089
    19. results suggest a Chk1-OGT-vimentin pathway that regulates the intermediate filament network during cytokinesis PMID: 29021254
    20. CHEK1-mediated DNA damage checkpoint has a role in the ESR2-NCF1-ROS pathway sensitization of esophageal cancer cells to 5-fluorouracil-induced cell death PMID: 27310928
    21. monitoring CHEK1 expression could be used both as a predictor of outcome and as a marker to select AML patients for CHK1 inhibitor treatments. PMID: 27625304
    22. PLAUR to be essential for activation of Checkpoint kinase 1 (CHK1); maintenance of cell cycle arrest after DNA damage in a TP53-dependent manner; expression, nuclear import and recruitment to DNA-damage foci of RAD51 recombinase, the principal protein involved in the homologous recombination repair pathway. PMID: 27685627
    23. Results unveil a new aspect of PERK function and previously unknown roles for Claspin and Chk1 as negative regulators of DNA replication in the absence of genotoxic stress. PMID: 27375025
    24. The findings reveal ATXN3 to be a novel deubiquitinase of Chk1, providing a new mechanism of Chk1 stabilization in genome integrity maintenance. PMID: 28180282
    25. These findings demonstrate an unsuspected requirement for a balanced nucleotide pool for optimal Chk1 activation both in unchallenged cells and in response to genotoxic stress. PMID: 27383768
    26. CHK1 overexpression is associated with T-cell and Hodgkin Lymphoma. PMID: 26988986
    27. Checkpoint kinase 1 and 2 signaling is important for apoptin regulation. PMID: 27512067
    28. Genetic variants of the CHEK1 gene are significantly related to overall survival and disease-free survival of esophageal squamous cell carcinoma patients. PMID: 27924519
    29. Role of the CHK1-RAD51 signaling pathway in osteosarcoma cells. PMID: 28000895
    30. High CHK1 expression is associated with increased radioresistance of non-small cell lung cancer. PMID: 27553023
    31. CHEK1 loss-of-function mutations have not been found in human tumors, and transgenic expression of Chek1 in mice promotes oncogene-induced transformation. [review] PMID: 26527132
    32. persistence of CHK1 levels in response to DNA damage in p53-deficient cancer cells, leads to CHK1-mediated activation of NF-kappaB and induction of NF-kappaB-regulated genes in cells and in associated tumor-derived microvesicles, both of which are abrogated by loss or inhibition of CHK1. PMID: 26921248
    33. Chk1's expression is controlled by p53 and RB/E2F1 at the transcriptional level. PMID: 26867682
    34. High CHK1 expression correlates with urinary bladder cancer. PMID: 26657501
    35. This study shows that Chk1 indeed maintains a closed conformation in the absence of DNA damage through an intramolecular interaction between a region (residues 31-87) at the N-terminal kinase domain and the distal C terminus. A highly conserved Leu-449 at the C terminus is important for this intramolecular interaction. PMID: 27129240
    36. Avoiding damage formation through invalidation of Mus81-Eme2 and Mre11, or preventing damage signaling by turning off the ATM pathway, suppresses the replication phenotypes of Chk1-deficient cells. PMID: 26804904
    37. Chk1 is a predictive biomarker of radiotherapy resistance and early local recurrence. PMID: 26459098
    38. A new pathway of proliferation restriction for tetraploid untransformed cells that seems to be specific for loss of adhesion dependent cytokinesis failure involves Chk1 and p53 activation during G2. PMID: 26693937
    39. Human induced pluripotent stem cells fail to activate CHK1 when exposed to DNA replication inhibitors and commit to apoptosis instead. PMID: 26810087
    40. Isolate/characterize mantle cell lymphoma cell line resistance to Chk1 inhibitor PF-00477736. PMID: 26439697
    41. Results support the inhibition of checkpoint kinase 1 (Chk1) as a new therapeutic strategy in acute lymphoblastic leukemia. PMID: 26542114
    42. these results demonstrate a positive feedback loop involving Rad9A-dependend activation of Chk1. PMID: 26658951
    43. DNA damage induces Chk1 phosphorylation on chromatin followed by releasing phospho-Chk1 from the chromatin into soluble nucleus and the cytoplasm where Chk1 activates the cell cycle checkpoints; and Chk1 is degraded and checkpoint signaling is terminated. PMID: 26296656
    44. nasopharyngeal carcinoma cells depend on CHK1 and WEE1 activity for growth PMID: 26025928
    45. Suppression of CHK1 by ETS Family Members Promotes DNA Damage Response Bypass and Tumorigenesis PMID: 25653093
    46. Report strong synergism observed by combining Chk1 and Wee1 inhibitors in preclinical models of mantle cell lymphoma. PMID: 25428911
    47. mutations targeting the putative Chk1 KA1 domain confer constitutive biological activity by circumventing the need for ATR-mediated positive regulatory phosphorylation. PMID: 26039276
    48. CHEK1 was a direct target of miR-195, which decreased CHEK1 expression in lung cancer cells. High expression of CHEK1 in lung tumors was associated with poor overall survival. PMID: 25840419
    49. Our findings suggest that the addition of CHEK1 inhibitor increases the response of ovarian cancer cells to TPT. Furthermore, reduced dosages of both drugs achieved maximal cytotoxic effects by combining TPT with CHEK1 inhibitor. PMID: 25884494
    50. these results suggest that breast cancer cells may rely on mTORC2-Chk1 pathway for survival PMID: 25460505

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  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily
  • 组织特异性：
    Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.
  • 数据库链接：

    HGNC: 1925

    OMIM: 603078

    KEGG: hsa:1111

    STRING: 9606.ENSP00000388648

    UniGene: Hs.24529