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CDH2 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA243509A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA243509A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    CDH2 is engaged in morphogenetic processes throughout the formation of cardiac and neural tissues, as well as osteogenesis, skeletal myogenesis, and vascular maturation during development. In adults, CDH2 is expressed by neural cells, endothelial cells, stromal cells, and osteoblasts, among other cell types, and is important for synapse function, vascular stability, and bone homeostasis. CDH2 plays a role in neurons, cardiac muscle, and cancer metastasis. Abnormal expression of CDH2 is a hallmark of EMT in many forms of solid tumors, culminating in the acquisition of an aggressive tumor phenotype.

    The main steps in the production of this CDH2 recombinant antibody include immunization; harvest of positive spleen cells; obtaining the antibody sequence by screening and sequencing; expression of the target antibody in mammalian cells; purification. The CDH2 antibody was produced recombinantly and has many advantages: high reproducibility, specificity and scalability. And has been validated in ELISA, IHC.

  • Uniprot No.：
    P19022
  • 基因名：
  • 别名：
    CDH2; CDHN; NCAD; Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD antigen CD325
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human N Cadherin
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1A9
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. Plays a role in cell-to-cell junction formation between pancreatic beta cells and neural crest stem (NCS) cells, promoting the formation of processes by NCS cells. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
  • 基因功能参考文献：
    1. Human keratinocyte cells reduce the N-cadherin levels of co-cultured melanoma cells. Cell-to-cell contact is necessary for this keratinocyte-mediated N-cadherin reduction. Keratinocytes reduce intracellular calcium in co-cultured melanoma cells. PMID: 29902459
    2. The FGFR4-388arg variant promotes lung cancer progression by N-cadherin induction. PMID: 29402970
    3. This study showed that the significant N-cadherin expression especially in high-grade meningioma group. PMID: 29297710
    4. Our experiments presented a new mechanism adopted by GDNF supporting glioma development and indicated a possible therapeutic potential via the inhibition of proN-cadherin/FGFR1 interaction. PMID: 29750313
    5. In patients with gastric cancer, the strong expression of N-cadherin in lymph nodes correlated with more lymph nodes metastasis, an advanced stage, and poor prognosis PMID: 28247164
    6. Regulatory networks specifying cortical interneurons from human embryonic stem cells reveal roles for CHD2 in interneuron development PMID: 29229852
    7. A study with nonsyndromic cleft lip with or without cleft palate (NSCL+/-P) cases (N=292) and controls (N=287) established association of a SNP in intron 2 of CDH2 with NSCL+/-P as a risk factor. PMID: 29524576
    8. Results show that N-cadherin is highly expressed in 70% of patients with glioma. However, N-cadherin, as a representative EMT marker, have limited prognostic value in glioma. Nonetheless, the EMT process in gliomas may be compounded by enhanced N-cadherin expression supported by unfavorable prognostic outcomes. PMID: 28851312
    9. Findings reveal the importance of CDH2-mediated cell contacts in preserving features of the juvenile nucleus pulposus cell phenotype. PMID: 27292569
    10. Tumor-promoting role of N-cadherin in thyroid cancer was closely related to the activities of the MAPK/Erk, the phosphatidylinositol-3-kinase (PI3K)/Akt and p16/Rb signaling pathways. PMID: 28042956
    11. Triple negative breast cancer cells surviving short-term chemotherapy treatment are more invasive than bulk tumor cells. Cell surface pro-N-cadherin expression is associated with the invasive and chemo-resistant behaviors of this tumor cell subset. PMID: 27768598
    12. Sec8 regulates N-cadherin expression by controlling Smad3 and Smad4 expression through CBP, thereby mediating the epithelial-mesenchymal transition. PMID: 27769780
    13. Results found that the N-glycan at N402 is comprised of beta 1,6 GlcNAc branching and that in glioma, deficient N402 N-glycosylation destabilises N-cadherin and leads to its proteasomal degradation. Destabilisation of N-cadherin inhibits cadherin-mediated cell-cell adhesion and promotes cell migration. Our findings imply that the control of N-cadherin stability by N-glycosylation is important in glioma migration. PMID: 27864899
    14. In adrenocortical carcinomas, the loss of N-cadherin is a frequent phenomenon while the existence of TERT promoter mutations is not, and nuclear telomerase expression is present in only a minority of cases. PMID: 27886397
    15. High CDH2 expression is associated with M2-type acute myeloid leukemia. PMID: 27064800
    16. Migration of bone marrow-mesenchymal stem cells in response to TGF-beta was mediated through N-cadherin and noncanonical TGF-beta signals. PMID: 28213973
    17. Studied the roles of MIRN145 in lung adenocarcinoma (LAC) and its targeting of N-cadherin. Knockdown of N-cadherin inhibited invasion and migration of LAC cell lines similar to overexpression of MIRN45. PMID: 28120164
    18. Genetic mutations in CDH2-encoded N-cadherin may represent a novel pathogenetic basis for arrhythmogenic cardiomyopathy. PMID: 28326674
    19. Extracellular and intracellular cleavage of N-cadherin might be involved in elevated MMP-9 expression enhancing tumor cell invasion. PMID: 27737648
    20. a mechanism where the membrane organization of CD82, through specific posttranslational modifications, regulates N-cadherin clustering and membrane density, which impacts the in vivo trafficking of AML cells. PMID: 26592446
    21. fluorescent imaging to demonstrate Ncad-mediated single cell responses to developmental cues within hydrogels towards chondrogenesis. PMID: 27106637
    22. Our data demonstrate that N-Cadherin was markedly overexpressed and miR-199b-5p was significantly downregulated in hepatocellular carcinoma (HCC). MiR-199b-5p exerts inhibitory effects on EMT, and directly targets N-cadherin in HCC, supporting the potential utility of miR-199b-5p as a promising strategy to treat HCC PMID: 28588321
    23. Study demonstrates a critical role of presynaptic cadherin/catenin/p140Cap cell adhesion complexes in stabilizing functional synapses and spines in the developing neocortex. PMID: 28641114
    24. investigated the in vitro tumor/metastasis suppressor effects of plakoglobin in ovarian cancer cell lines with mutant p53 expression and different cadherin profiles PMID: 27144941
    25. These results uncover a new role for p120 catenin bound to the N-cadherin precursor ensuring its trafficking through the biosynthetic pathway towards the cell surface. PMID: 27254316
    26. Finding that the cells expressing N-cadherin gave rise to tumors with no expression of N-cadherin is in agreement with the classical view of epithelial to mesenchymal transition. Epithelial to mesenchymal transition and N-cadherin are associated with dissemination and not with the ability to establish new tumor growth. PMID: 27224422
    27. These data implicate CDH2 mutations as novel genetic causes of Arrhythmogenic Right Ventricular Cardiomyopathy and contribute to a more complete identification of disease genes involved in cardiomyopathy. PMID: 28280076
    28. High expression of N-cadherin is associated with bladder cancer. PMID: 27683053
    29. Snail and N-cadherin are constitutively and inducibly expressed in papillary thyroid carcinoma PMID: 26219900
    30. SFMSCs increased through upregulation of the activated lymphocyte cell adhesion molecule (ALCAM) and N-cadherin by microRNA-192 and -218 downregulation, similar to BMMSCs and ADMSCs. PMID: 28039611
    31. the synergy between N-cadherin and FGFR signalling that ensure cellular reorganization during cell movements, mainly during cancer cell migration and metastasis but also during developmental processes. PMID: 27320194
    32. Underexpression of CDH2 is associated with adrenocortical tumors. PMID: 27468715
    33. Of the seven polymorphisms, two reached statistical significance for obsessive-compulsive disorder under additive and codominant models of inheritance PMID: 26093892
    34. Targeting N-cadherin may be a promising therapeutic approach, particularly in cisplatin-resistant, therapy refractory and metastatic Germ cell tumor. PMID: 26451610
    35. Metformin's anti-cancer therapeutic effect is mediated through different molecular mechanism in wild-type vs. deficient N-cadherin cancer cells. PMID: 26359363
    36. Expression data of NCAD shows no association with advanced gastric cancer brain metastasis. PMID: 26260219
    37. CDH2 was found to be a susceptibility gene for Gilles de la Tourette syndrome in a Danish cohort. PMID: 26032459
    38. Existing knowledge regarding the role of CDH2 and CDH11 during development and differentiation in vivo and in vitro is reviewed. [review] PMID: 25771201
    39. N-cadherin was widely expressed in CRC cell lines and silencing of N-cadherin suppressed the proliferation and migration of the CRC cell line HT-29 by upregulating E-cadherin, suggesting a potential role of N-cadherin in inducing EMT. PMID: 25936636
    40. N-cadherin and connexin 43 expression in in group of diffuse astrocytomas and anaplastic astrocytomas may be evidence for their role in tumor formation and progression PMID: 25386667
    41. Foxn3 is a direct transcriptional suppressor of N-cadherin in colorectal metastasis tissues. PMID: 26069251
    42. the expression of N-cadherin was associated with Vasculogenic mimicry formation in esophageal squamous cell carcinoma PMID: 25575439
    43. Results suggest that N-cadherin may promote motility and invasiveness through distinct mechanisms and that beta-catenin may be an integral mediator of N-cadherin-dependent invasive signaling in oral epithelia. PMID: 25175499
    44. Results demonstrate that miR-194 affected the growth and metastasis of osteosarcoma cells both in vitro and in vivo suggesting that miR-194 functions as tumor suppressor gene probably by downregulating CDH2 and IGF1R. PMID: 25096247
    45. association of beta-catenin with N-cadherin is regulated by actin polymerization during contractile activation PMID: 25713069
    46. Decreased N-cadherin expression is linked to increased ADAM-10 expression in atherosclerotic lesions. PMID: 24985126
    47. This indicates that similar biofunctionalization approaches based on N-cadherin and L1 can be translated to 3-D "transplantable" scaffolds with enhanced neurotrophic behaviors. PMID: 24914828
    48. miR-199a targeted the sequence within the 3'UTR of the N-cadherin mRNA and suppressed the TGF-beta1-induced increase in the protein level of N-cadherin in a manner independent of SNAI1. PMID: 25041364
    49. N-cadherin and CD133 expressions are strongly correlated and N-cadherin appears as a potential breast cancer metastases marker in a specific patient subpopulation. PMID: 24962344
    50. High N-cadherin expression is associated with malignant bone and soft tissue tumors. PMID: 23799912

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell membrane, sarcolemma. Cell junction. Cell surface.
  • 数据库链接：

    HGNC: 1759

    OMIM: 114020

    KEGG: hsa:1000

    STRING: 9606.ENSP00000269141

    UniGene: Hs.464829