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CDC45 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA005020A0HU
  • 规格：
    ￥1320
  • 其他：

产品详情

  • 产品描述：

    The CDC45 recombinant monoclonal antibody is prepared by cloning the gene fragment encoding human CDC45 protein into the expression vector and subsequently transfecting clones into the cell line for in vitro expression. This CDC45 antibody can detect the endogenous content of the human CDC45 protein. It is purified by the affinity chromatography method. And it has been validated in the ELISA application.

    DNA replication protein CDC45 is an integral part of the eukaryotic CMG (Cdc45-MCM-GINS) replicative helicase. CDC45 functions with the replisome CMG helicase where minichromosome maintenance (MCM2–7) proteins provide motor activity for unwinding duplex during replication. It plays an essential role in initiating chromosomal DNA replication.

  • Uniprot No.：
    O75419
  • 基因名：
  • 别名：
    CDC 45 antibody; CDC 45L antibody; CDC45 (cell division cycle 45; S.cerevisiae homolog) like antibody; CDC45 antibody; CDC45 cell division cycle 45 like antibody; CDC45 like antibody; CDC45 related protein antibody; CDC45_HUMAN antibody; CDC45L2 antibody; Cell division control protein 45 homolog antibody; Cell division cycle 45 like 2 antibody; Cell division cycle 45 like antibody; PORC PI 1 antibody; PORC-PI-1 antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CDC45
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4B4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Required for initiation of chromosomal DNA replication.
  • 基因功能参考文献：
    1. The size and relative position of the three helices (alpha2, alpha7 and alpha8) that contribute to the active site architecture are highly conserved between Cdc45 and RecJ, whereas the helical structure on the solvent-exposed DHH side (alpha3, alpha4 and alpha5) shows more conformational variability. PMID: 27189187
    2. strong evidence that Cdc45 directly loads RPA on the emerging nascent ssDNA PMID: 28100698
    3. The oncogenic activity of cell division cycle 45 was also confirmed in vivo. In conclusion, cell division cycle 45 may serve as a novel biomarker and a potential therapeutic target for papillary thyroid cancer. PMID: 28474999
    4. Mutations in CDC45, Encoding an Essential Component of the Pre-initiation Complex, Cause Meier-Gorlin Syndrome and Craniosynostosis PMID: 27374770
    5. ectopic expression of Cdc45 led to increased firing of replication origins, severe replication stress, an early S-phase arrest, replication fork stalling, and eventually cell death by apoptosis. PMID: 26919204
    6. The depletion of HDHB from human cells diminishes Cdc45 association with chromatin, suggesting that HDHB may facilitate Cdc45 recruitment at G1/S in human cells. PMID: 25933514
    7. The findings suggest that hCdc45 not only binds to but also slides on DNA with a 3'-5' polarity and, thereby acts as a molecular 'wedge' to initiate DNA strand displacement. PMID: 24293646
    8. Data suggest that CDC45 and MCM10 (minichromosome maintenance complex component 10) directly interact and establish a mutual co-operation in DNA binding; key domains appear to interact and then interact with DNA inside cells or in cell-free systems. PMID: 23750504
    9. CDC45 directly interact with MCM2-7 proteins; with PSF2, PSF3 and SLD5 in GINS subunits; and with replication protein A2, AND-1. A considerable portion of CDC45 localizes in a region other than the DNA replication forks in nuclei. PMID: 23364835
    10. After UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage. PMID: 22536402
    11. Data show that using a 200-nt primed circular DNA substrate, the combined action of DNA polymerase epsilon and the Cdc45/Mcm2-7/GINS (CMG complex) leads to the formation of products >10 kb in length. PMID: 22474384
    12. Structural and functional insights into the DNA replication factor Cdc45 reveal an evolutionary relationship to the DHH family of phosphoesterases PMID: 22147708
    13. Cdc45, which is known to be required for replication fork progression, shows similar patterns of origin association to those of Timeless PMID: 20124417
    14. The coordinated binding of DUE-B and Cdc45 to origins and the physical interactions of DUE-B, Cdc45, and TopBP1 suggest that complexes of these proteins are necessary for replication initiation. PMID: 20065034
    15. the Chk1-mediated S-phase checkpoint targets initiation factor Cdc45 via a Cdc25A/Cdk2-independent mechanism PMID: 16912045
    16. CDC45L is part of the GINS complex. PMID: 17557111
    17. Cdc45 may play an important role in elongation of DNA replication by bridging the processive DNA polymerases delta and epsilon with the replicative helicase in the elongating machinery PMID: 17573775
    18. Cdc45 expression is tightly associated with proliferating cell populations and Cdc45 seems to be a promising candidate for a novel proliferation marker in cancer cell biology PMID: 17608804
    19. Thus, the evidences for ubiquitylation of Cdc45 refer the first posttranslational modification of this essential replication factor. PMID: 17767920
    20. human TopBP1 is involved in the formation of the initiation complex of replication in human cells and is required for the recruitment of Cdc45 to origins of DNA replication PMID: 17887956
    21. Results suggest that apoptosis induced by replication inhibitors in Chk1-depleted cells is dependent upon the helicase cofactor Cdc45. PMID: 18239674
    22. CDT1 associates with CDC7 and recruits CDC45 to chromatin during S phase PMID: 19054765
    23. interactions between Cdc45, Mcm2-7, and the GINS complex (collectively called the CMG complex), which seem to play a key role in the formation and progression of replication forks PMID: 19805216

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    CDC45 family
  • 组织特异性：
    Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
  • 数据库链接：

    HGNC: 1739

    OMIM: 603465

    KEGG: hsa:8318

    UniGene: Hs.474217