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  4. CD4 Recombinant Monoclonal Antibody

CD4 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA004935A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in Jurkat whole cell lysate
      All lanes CD4 antibody at 0.8μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 55 KDa
      Observed band size: 55 KDa
    • IHC image of CSB-RA004935A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA004935A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品描述：

    The CD4 recombinant monoclonal antibody is a highly specific antibody against the human CD4 protein. This CD4 antibody was produced in vitro by transfecting human CD4 monoclonal antibody gene- vector clones into the cell line, followed by affinity-chromatography purification from the tissue culture supernatant (TCS). The mice were immunized with a human CD4 synthetic peptide to produce the human CD4 monoclonal antibody. This CD4 antibody has the same isotype as rabbit IgG. It can be applied in ELISA, WB, and IHC.

    T cells, natural killer (NK) cells, monocytes, and macrophages all express the CD4 coreceptor. CD4 regulates differentiation, migration, and cytokine expression in macrophages and NK cells. It plays a role in thymic development and antigen recognition in the periphery in T lymphocytes.

  • Uniprot No.：
    P01730
  • 基因名：
  • 别名：
    CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD antigen CD4
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    2B7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:500
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.; (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1). Down-regulated by HIV-1 Vpu. Acts as a receptor for Human Herpes virus 7/HHV-7.
  • 基因功能参考文献：
    1. CD4 receptor levels are very low in THP-1 differentiated cells and that this down-regulation of the virus receptor is the result of miR-221/miR-222 up-regulation during differentiation. In THP-1 cell line stably expressing a modified CD4 that is not modulated by miR-221/miR-222, productive HIV-1 infection occurs after cell differentiation. PMID: 29301198
    2. Human BD-2 and BD-3 play a positive and negative regulatory role in development and proliferation of human effector CD4+ T cells which is essential for optimal adaptive immune responses and the control of immunopathology. PMID: 30098283
    3. These findings highlight regions of cross talk between gp120 and gp41 and identify heptad repeat region 1(HR1) residues that play important roles in regulating CD4-induced conformational changes in Env. PMID: 29875245
    4. CD4(+) and CD19(+) peripheral lymphocytes of early stage AD patients exhibit mitochondrial depletion, as seen both at the level of DNA and protein PMID: 28923392
    5. Human microRNAs-221 and -222 inhibit HIV-1 entry in macrophages by targeting the CD4 viral receptor. PMID: 28978468
    6. CD4 has four ecto-domains (D1-D4), D1, D2, and D4 each contain a distinctive disulfide bond. Reduction of D2 disulfide decreases the dynamics of its surrounding beta-strands. Favorable inward collapse of structure occurs around the D2 disulfide after reduction. PMID: 29470989
    7. Our results indicate that CD4 expression and older age are adverse prognostic factors in wild-type NPM1, FLT3-ITD-negative CN-AML. PMID: 28318150
    8. We investigated the prevalence, magnitude and phenotype of CTAg-specific T cells in the blood of patients with testicular germ cell tumors (TGCTs). CD8(+) and CD4(+) T-cell responses against MAGE-A family antigens were present in 44% (20/45) of patients' samples assayed by ex vivo IFN-gamma ELISPOT. Spontaneous T-cell immunity against CTAg proteins therefore develops in many patients with testicular cancer. PMID: 28555838
    9. Depletion of the gamma2 or mu1A (AP1M1) subunits of AP-1, but not of gamma1 (AP1G1), precludes Nef-mediated lysosomal degradation of CD4. PMID: 27909244
    10. findings provide a mechanistic explanation for previous observations that dimerization-defective Nef mutants fail to down-regulate CD4 and validate the Nef dimerization interface as a target site for antiretroviral drug development PMID: 28031466
    11. Mouse leukemia cell lines that could express hCD4 and CCR5 were thus established to facilitate normal entry of HIV-1 so that a human CD4/CCR5 transgenic mice cell model can be used to investigate the transmission and pathogenesis of HIV/AIDS and potential antiviral drugs against this disease. PMID: 28028680
    12. The percentage of lamina propia CD4+LAP+ cells is increased in active ulcerative colitis, showing reduced suppressor activity due to their increased proportion of intracellular IL-17 expression. PMID: 26589955
    13. possible therapeutic targets for childhood severe asthmatics identified thru DNA microarray PMID: 25979195
    14. The study gives insights into the role of CD4 on cell membrane mechanical characteristics. PMID: 26362701
    15. A decrease of CD4(+) CD25(+) CD127(low) FoxP3(+) regulatory T cells with impaired suppressive function had been found in untreated ulcerative colitis patients. PMID: 26333292
    16. Redox shuffling of the allosteric disulfide results in previously undescribed conformational changes in CD4 that are likely important for its interaction with its protein partners. PMID: 27009680
    17. Increased levels of activated and highly susceptible HIV-1 target cells, reduced CD4 and enhanced CXCR4 cell surface expression, together with the high susceptibility to FAS-induced programmed cell death may contribute to the rapid CD4+ T cell depletion. PMID: 26452480
    18. HRB knock-down affected CD4 downregulation induced by Nef but not by HIV-1 Vpu. PMID: 26701340
    19. Increased CD4, IL-17, and COX-2 expression are associated with subclinical inflammation in malar melasma. PMID: 26381025
    20. CD4 receptor induced HIV size expansion prior to cell entry. PMID: 26432024
    21. Sustained expression of CD83 was observed when CD4+ T cells were induced by transforming growth factor-beta to differentiate into CD4+CD25+ forkhead box P3+ regulatory T (iTreg) cells. PMID: 25997495
    22. CHD in Chinese population is strongly associated with HLA-DRB1*01 and DRB1*04 haplotypes, and formation of CD4(+)CD28(null) T cells was related to HLA-DRB1*01, DRB1*04, and DRB1*15 alleles. PMID: 20842443
    23. These results lead to a model for the docking of the full AP-2 tetramer to membranes as bound to Nef, such that the cytosolic tail of CD4 is situated to interact with its binding site on Nef. PMID: 24473078
    24. This study demonstrates a null association between the CD4 C868T polymorphism and an individual's susceptibility of HIV-1 acquisition in a Chinese population. PMID: 25611551
    25. Nicotine ameliorates experimental severe acute pancreatitis via enhancing immunoregulation of CD4+ CD25+ regulatory T cells. PMID: 25742430
    26. T-cell receptor activation of human CD4(+) T cells shifts the innate toll-like receptor response from CXCL8(hi) IFN-gamma(null) to CXCL8(lo) IFN-gamma(hi). PMID: 26205220
    27. Nef domains involved in CD4 downregulation were necessary for activation of plasmacytoid dendritic Cell. PMID: 25972534
    28. Here, we describe the HIV care cascade and ART delivery supply chain to examine how mathematical modeling can provide insight into cost-effective strategies for scaling-up ART coverage in sub-Saharan Africa and help achieve universal ART coverage. PMID: 25249293
    29. Rapidly translating new science into policy is a critical component of the HIV response. Adapting and implementing the 2013 WHO treatment recommendations are necessary to prevent unnecessary illness, death, HIV transmission and costs. PMID: 25266850
    30. To augment the literature, we present a review of ART adherence interventions from 2013 to the present. We included peer-reviewed journals as well as abstracts from two key conferences. PMID: 25304006
    31. Earlier access of key populations to combination prevention interventions, combined with sustained political commitment and supportive environment for key populations, are essential for maximum impact of ART on the HIV epidemic in Vietnam. PMID: 25472886
    32. The study shows that activated CD4+ T cells can produce 1,25(OH)2D3, and that 1,25(OH)2D3 induces a 2-fold up-regulation of the VDR protein expression in activated CD4+ T cells by protecting the VDR against proteasomal degradation. PMID: 24792400
    33. Data indicate that CD4 antigen binding disrupts quaternary interactions at the HIV-1 Env trimer apex. PMID: 24931470
    34. the multi-faceted role of human SP-D against HIV-1 PMID: 25036364
    35. The effects of HIV Nef protein on down regulation of CD4 and HLA class I in patients with early and chronic HIV infection with HIV-1 subtype C is reported. PMID: 25193656
    36. The current review contributes to a better understanding of the role of AhR and its signaling pathway in CD4 helper T cell mediated inflammatory disorder. [review] PMID: 24905409
    37. s determined that the activation of CD4 via interaction with major histocompatibility complex class II (MHC-II) triggers cytokine expression and the differentiation of human monocytes into functional mature macrophages. PMID: 24942581
    38. HIV-1 Nef interacts with Alix in late endosomes, and this is required for efficient lysosomal targeting of CD4. PMID: 25118280
    39. Human tumor-infiltrating CD4+CD69+ T cells suppress T cell proliferation via membarene -bound TGF-beta1. PMID: 24668348
    40. These results demonstrate that aptamer-facilitated cell specific delivery of shRNA represents a novel approach for efficient RNAi delivery and is potentially to be developed for therapeutics targeting specific T cells subtypes. PMID: 25241192
    41. data show that Ag-specific CD4(+) CD25(+) CD134(+) CD39(+) T cells are highly enriched for Treg cells, form a large component of recall responses and maintain a Treg-cell-like phenotype upon in vitro expansion. PMID: 24752698
    42. Binding of the HIV-1 envelope protein gp120IIIB to the CD4/CXCR4/CCR5 heterooligomer was negligible, and the gp120-induced cytoskeletal rearrangements necessary for HIV-1 entry were prevented. PMID: 24778234
    43. Using biopsies from H. pylori-positive patients, PCR was used to quantify the expression of Foxp3 mRNA and IHC was used to semi- quantify the number of CD4+ CD25+ T cells in gastric mucosa. PMID: 24901172
    44. HIV glycoprotein 120 (gp120) requires disulfide reduction in CD4 domain 1 or 2 which impairs thioredoxin-driven CD4 dimerization PMID: 24550395
    45. this study identifies a novel mechanism of Chronic heat stress immunosuppression mediated by regulating CD4 CD25 Foxp3 Tregs. PMID: 24151582
    46. The acquisition of B cell stimulating properties by naive cord blood CD4 T cells required the STAT3-dependent expression of ICOS and IL-21. PMID: 23923047
    47. CD4 retro-translocates with oxidized intrachain disulfide bridges, and only upon proteasomal inhibition does it accumulate in the cytosol as already reduced and deglycosylated molecules. PMID: 24257748
    48. CD4(+)CD25(+)FOXP3(+) Treg levels were lower in Kawasaki disease patients than healthy subjects, and levels before treatment were lower in intravenous immunoglobulin (IVIg)-resistant patients than in IVIg-sensitive patients. PMID: 23340699
    49. The CD4(372-433) peptide fragment in the given sample undergoes some rotational averaging of anisotropic interactions; additional amino acid type-specific assignments are achieved for 10 amino acid spin systems for both CD4(372-433) and Vpu. PMID: 23863698
    50. sCD4 might be considered an important parameter for RA disease progression with potential diagnostic importance. PMID: 23700441

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts (PubMed:12517957, PubMed:9168119). Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • 组织特异性：
    Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells
  • 数据库链接：

    HGNC: 1678

    OMIM: 186940

    KEGG: hsa:920

    STRING: 9606.ENSP00000011653

    UniGene: Hs.631659