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CCR2 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA297464A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA297464A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    CCR2 is predominantly expressed by monocytes/macrophages with strong proinflammatory functions, regulating the mobilization of monocytes from bone marrow to the inflammatory sites. CCR2 plays important roles in the extravasation and transmigration of monocytes under inflammatory conditions. CCL2/CCR2 signaling is best known for its role in regulating macrophage recruitment and polarization during inflammation. CCR2 deficiency markedly reduces Ly6Chi monocytes trafficking out of bone marrow and to sites of inflammation. CCR2 deficiency also reduces Th1 response and the severity of experimental autoimmune diseases.

    The recombinant CCR2 antibody production commenced with the obtaining of genes encoding antibody against CCR2. Antibody genes were obtained by sequencing and screening DNA reversely transcribed from RNA that was extracted from the B cells isolated from immunized animals. These genes were cloned into plasma vectors and subsequently transfected into a mammalian cell line for production. The product is the recombinant CCR2 antibody. It underwent purification using Affinity-chromatography from the cell culture medium. This recombinant CCR2 antibody has been validated to detect the CCR2 protein from Human in the ELISA, IHC.

  • Uniprot No.：
    P41597
  • 基因名：
  • 别名：
    CCR2; CMKBR2; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; Monocyte chemoattractant protein 1 receptor; MCP-1-R; CD antigen CD192
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CCR2/CKR2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    6A1
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Key functional receptor for CCL2 but can also bind CCL7 and CCL12. Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (Probable). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B. Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation. Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B. Mediates the recruitment of macrophages and monocytes to the injury site following brain injury.; (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
  • 基因功能参考文献：
    1. miR-135a-3p serves as a tumour suppressor gene in ovarian cancer by regulating CCR2. PMID: 30138893
    2. this study shows that CCR2 is upregulated on peripheral T cells in osteoarthritis PMID: 30403025
    3. The A allele of CCR2 rs1799864 was associated with a higher MCP-1 level in AD and MCI patients. PMID: 29352259
    4. Study results revealed that CCR2 promotes epithelialtomesenchymal transition through MMP2 in liver cancer. PMID: 30132565
    5. NOX4 is induced in early alcoholic liver injury and regulates CCR2/CCL2 mRNA stability thereby promoting recruitment of inflammatory cells and production of proinflammatory cytokines. PMID: 28383062
    6. T cell-stimulated CLL cells actively recruited monocytes; recruitment critically depended on the C-C-motif-chemokine-receptor-2 axis. PMID: 28971904
    7. Study found that the activation of CCR2 by its ligand CCL2 increased the expression of SMO and Gli-1, resulting in Hh pathway activation, epithelial-mesenchymal transition and hepatocellular carcinoma cell invasion. PMID: 29115520
    8. results confirmed that the CCR2 3'UTR acts as a metastasis suppressor by acting as a ceRNA for STARD13 and thus inhibiting RhoA-ROCK1-MLC-F-actin pathway in breast cancer cells. PMID: 28818997
    9. It can be concluded that MCP-1 and CCR2 polymorphisms are not associated with AgP in Turkish population. PMID: 28458180
    10. beta-arrestin2/AP-1-dependent beta2AR signaling has a critical role in the regulation of CCR2 expression and recruitment of leukocytes to the heart following injury PMID: 27956622
    11. Results indicated that MCP-1 and CCR2 polymorphisms may influence the progression of IgAN, but not increase/decrease its susceptibility PMID: 27788494
    12. High CCR2 expression is associated gastric cancer. PMID: 26992207
    13. Our data strongly support the use of CCR2 and CD180 mRNAs as whole blood pharmacodynamic (PD)biomarkers for BRD4 inhibitors, especially in situations where paired tumor biopsies are unavailable. In addition, they can be used as tumor-based PD biomarkers for hematologic tumors. PMID: 28073847
    14. Through experiments with chimeras of MCP-1 and MCP-3, we identified the chemokine amino-terminal region as being the primary determinant of both the binding and signaling selectivity of these two chemokines at CCR2. PMID: 28536301
    15. Comprehensive Computational Analysis of GWAS Loci Identifies CCR2 as a Candidate Gene for Celiac Disease Pathogenesis PMID: 28059456
    16. Studies indicate manipulating CCL2-CCR2 interaction as a potential approach for combating metastatic disease. PMID: 26885690
    17. Interruption of either CCL2-CCR2 signaling or cathepsin B function significantly impaired perineural invasion (PNI). PMID: 28951461
    18. No statistically significant correlation was found between CRC and the 190 G/A CCR2 polymorphism with colorectal cancer prevalence PMID: 29154245
    19. rs1799864 polymorphism significantly associated with occurrence risk of psoriasis vulgaris in Chinese population PMID: 29145242
    20. CCR2 genetic variants were not associated with risk of atherosclerotic coronary heart disease and glucometabolic traits. PMID: 27013693
    21. These data suggest that CCR1/CCR2B could be involved in clearing EBV-infected latency III B cells in immunocompetent individuals via directing the migration of these cells and attracting the chemokines-expressing immune cells. PMID: 28892735
    22. systemic mRNA expression of CCR2 strongly differentiated children who failed to achieve asthma control with triamcinolone administration PMID: 27665382
    23. Basophil migration into skin lesions of Systemic Lupus Erythematosus patients were observed, but not in normal skin tissue. This migration was related to the upregulation of chemokine receptors CCR1 and CCR2 on basophils. PMID: 28954264
    24. The CCR2 (+190 G/A) GG genotype frequencies for patients were significantly higher in the stage III-IV cancer group ( p = 0.036), and A allele carriers were significantly higher in the stage I-II ovarian cancer group. PMID: 28650671
    25. tumor-promoting role for CCL2 acting through CCR2 on the tumor microenvironment PMID: 27820834
    26. The findings of the work indicate a role for Galphaq and/or Galpha14 and in CCR2a/CCR2b-stimulated Rho A GTPase-mediated serum response factor activation. PMID: 26823487
    27. Endogenous TRAIL/TRAIL-R-mediated CCL2 secretion promotes accumulation of tumor-supportive immune cells in the cancer microenvironment. PMID: 28212753
    28. CCL2/CCR2 axis is expressed in the majority of monocytoid Acute myeloid leukemia blasts PMID: 28045930
    29. CCR2B and beta2AR signals to FLNa to stimulate its endocytosis and recycling to the plasma membrane. PMID: 27909248
    30. this study shows that CYTL1 possesses chemotactic activity and demonstrates that its functional receptor is CCR2B PMID: 27084102
    31. PCSK9 regulates monocyte CCR2 expression and chemotaxis. PMID: 28232185
    32. CCR2-targeted therapy with PF-04136309 in combination with FOLFIRINOX is safe and tolerable PMID: 27055731
    33. Binding of J113863 to CCR2 or CCR5 also induced the recruitment of beta-arrestin 2, whereas UCB35625 did not. UCB35625 induced the chemotaxis of L1.2 cells expressing CCR2 or CCR5. In contrast, J113863 induced the migration of L1.2-CCR2 cells but antagonized the chemokine-induced migration of L1.2-CCR5 cells. PMID: 27895119
    34. CCL2 suppressed HDL internalization and cholesterol efflux via CCR2 induction and p42/44 MAPK activation in ECs. CCL2 induction may contribute to impair HDL function and form atherosclerosis in coronary artery disease. PMID: 27458015
    35. High levels of TRAIL-R3 and CCR-2 expression in tumor epithelial cells identified patients with early breast cancer with poor outcomes. PMID: 28420351
    36. X ray crystal structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists PMID: 27926736
    37. Ingestion of an amino acid supplement immediately post-exercise appears to help maintain plasma MCP-1 concentrations and augment CCR2 expression in resistance trained men. PMID: 27384580
    38. HUMSCs expressing ISZ-sTRAIL might be an efficient therapeutic approach against lung cancer and MCP-1/CCR2 axis is essential for the tumor tropism of HUMSCs PMID: 26733169
    39. The s demonstrate a positive correlation between CCR2 receptor gene polymorphism and a subgroup of breast cancer showing poor prognosis, which deserves further investigation in larger samples for validation. PMID: 25716470
    40. Results of this study indicated that the CCL2-CCR2 axis was involved in the pathogenesis of oral lichen planus and was positively correlated with disease activity. PMID: 25893607
    41. sCCL2 production possibly will be associated with the adiposity and polymorphic phenotypes of CCL2 and CCR2, in Mexican-Mestizos with insulin resistance PMID: 26839895
    42. Increased CCR2 expression is associated with bladder cancer metastasis. PMID: 26556868
    43. These findings suggested that the CCL2/CCR2 chemokine signaling may regulate the epithelial-mesenchymal transition. PMID: 26648448
    44. CCR2 gene polymorphism (64I allele carriage) is a predisposing factor for allergy. PMID: 27534043
    45. data suggest that the CCR2-64I polymorphism is associated with the protective effect to development of cervical lesions as well as in the protection to HPV 16 infection. PMID: 26982176
    46. Ovarian cancer cells express both Hp and its receptor CCR2 but only after exposure to ascitic fluid, inducing moderated cell migration; results suggest that Hp-induced changes in the cell morphology, actin cytoskeleton structure, and migration ability of tumor cells, is possibly "preparing" these cells for the potential induction of the metastatic phenotype. PMID: 26211665
    47. The frequencies of the rare alleles of CCR2, ITGB3, and 3'UTR of c-fms in the Old Believers are lower than in the sample of Novosibirsk Russians, and the rare allele of DBH is more frequent PMID: 27239844
    48. Among HIV-seropositive samples 3.4% were homozygous CCR2-64I mutants, 17.8%) were heterozygous,78.8% were CCR2 wild type. PMID: 26300579
    49. results suggest that senescent dermal fibroblasts recruit stem cells by secreting various factors and that the CCL2/CCR2 axis is one of the mechanisms underlying this phenomenon PMID: 25808810
    50. Association of hydrogen sulfide with alterations of monocyte chemokine receptors, CCR2 and CX3CR1 in patients with coronary artery disease PMID: 26123579

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Expressed by monocytes and IL2-activated NK cells.
  • 数据库链接：

    HGNC: 1603

    OMIM: 601267

    KEGG: hsa:729230

    STRING: 9606.ENSP00000292301

    UniGene: Hs.511794