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  4. CCL19 Recombinant Monoclonal Antibody

CCL19 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA859522A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA859522A0HU diluted at 1:155 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA859522A0HU diluted at 1:155 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • 其他：

产品详情

  • 产品描述：

    CUSABIO cloned CCL19 antibody-coding genes into plasma vectors and then transfected these vector clones into mammalian cells using a lipid-based transfection reagent. Following transient expression, the recombinant antibodies against CCL19 were harvested and characterized. The recombinant CCL19 antibody was purified by affinity-chromatography from the culture medium. It can be used to detect CCL19 protein from Human in the ELISA, IHC.

    Chemokine CC ligand 19 (CCL19), which is also named as macrophage inflammatory protein 3-beta (MIP-3b), mediates various cellular behaviors by binding to CCR7. Recent articles have indicated that the CCL19/CCR7 axis can promote tumor progression. Nevertheless, some other studies indicated that CCL19 can modulate anti-tumor responses in lung cancer and ovarian cancer. previous studies demonstrated that CCL19 inhibited tumorigenesis, metastasis and angiogenesis, and the expression of CCL19 was associated with the prognosis of CRC patients.   CCL19 can inhibit the ERK/Elk-1 pathway, which is accompanied by the suppression of HIF-1α and VEGF-A. CCL19 is a critical regulator of the induction of T cell activation, immune tolerance, and inflammatory responses during continuous immune surveillance, homeostasis, and development. Local injection of CCL19-expressing mesenchymal stem cells augments the therapeutic efficacy of anti-PD-L1 antibody by promoting infiltration of immune cells. CCL19 with CCL21-tail displays enhanced glycosaminoglycan binding with retained chemotactic potency in dendritic cells.

  • Uniprot No.：
    Q99731
  • 基因名：
  • 别名：
    Beta chemokine exodus 3 antibody; Beta-chemokine exodus-3 antibody; C C chemokine ligand 19 antibody; C-C motif chemokine 19 antibody; CC chemokine ligand 19 antibody; CCL 19 antibody; CCL19 antibody; CCL19_HUMAN antibody; Chemokine (C C motif) ligand 19 antibody; Chemokine (CC motif) ligand 19 antibody; Chemokine C C Motif Ligand 19 antibody; Chemokine CC Motif Ligand 19 antibody; CK beta 11 antibody; CK beta-11 antibody; CKb 11 antibody; CKb11 antibody; EBI 1 ligand chemokine antibody; EBI1 ligand chemokine antibody; ELC antibody; Epstein-Barr virus-induced molecule 1 ligand chemokine antibody; Exodus 3 antibody; Exodus3 antibody; Macrophage inflammatory protein 3 beta antibody; MGC34433 antibody; MIP 3 beta antibody; MIP 3B antibody; MIP-3-beta antibody; MIP-3b antibody; MIP3 beta antibody; MIP3B antibody; OTTHUMP00000000531 antibody; SCYA 19 antibody; SCYA19 antibody; Small inducible cytokine A19 antibody; Small inducible cytokine subfamily A (Cys Cys) member 1 antibody; Small-inducible cytokine A19 antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CCL19
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3A12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
  • 基因功能参考文献：
    1. these data indicate CCL19/CCR7 contributes to proliferation and invasion of ESCs, which are conducive to the pathogenesis of endometriosis through activating PI3K/Akt pathway PMID: 28856757
    2. The research findings demonstrate for the first time that the chemokines CCL19, CCL21 and CCR7 play important roles in bone destruction by increasing osteoclast migration and resorption activity, and that has been linked to rheumatoid arthritis pathogenesis. PMID: 28729639
    3. breast cancers-derived soluble factors increase the migration of DCs toward CCL19. PMID: 27451948
    4. The migratory index to the CCR7 ligands, CCL19 and CCL21, was higher in T-cells from donors whose recipients will develop GvHD. PMID: 28112745
    5. Study confirmes that CCL19 induces the invasion and migration of breast cancer cells through the expression of markers of epithelial-mesenchymal transition. PMID: 28378417
    6. findings connect NOTCH1, DUSP22, and CCL19-driven chemotaxis within a single functional network, suggesting that modulation of the homing process may provide a relevant contribution to the unfavorable prognosis associated with NOTCH1 mutations in CLL. PMID: 28017968
    7. Deletion of this extended C-terminus reduces CCL21's affinity for heparin and transferring the CCL21 C-terminus to CCL19 enhances heparin binding mainly through non-specific, electrostatic interactions PMID: 27338641
    8. CCL19 is significantly overexpressed in patients with unstable carotid atherosclerotic plaques and may be a possible novel biomarker for identifying high-risk patients in whom more urgent intervention may be indicated. PMID: 27498092
    9. CrkL regulates CCL19 and CCR7-induced epithelial-to-mesenchymal transition via ERK signaling pathway in epithelial ovarian carcinoma patients. PMID: 25636509
    10. The solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. PMID: 26115234
    11. Our findings serve to elucidate the molecular mechanisms underlying the resistin induction of CCL19 expression in ECs and the shear-stress protection against this induction. PMID: 25656506
    12. The mRNA levels of CCL19/CCL21 in ankylosing spondylitis hip ligament were significantly higher than in osteoarthritis ligament. PMID: 25260647
    13. Serum CCL19 and CCL21 were up-regulated during Rickettsia conorii infection. PMID: 24507453
    14. CCL19 promoted monocyte adhesion to HUVEC cells. CCL19 rs2227302 was associated with coronary artery disease in a Chinese Han population. PMID: 24990231
    15. CCL19 is an antimicrobial protein with bacteriocidal activity against E. coli. PMID: 12949249
    16. CCL19 (rather than CCL21) may contribute to the accumulation of dendritic cells and macrophages in the inflamed lungs of patients with eosinophilic pneumonia. PMID: 24111618
    17. CIP4 is expressed at abnormally high levels in CLL cells, where it is required for CCL19-induced chemotaxis. PMID: 23644527
    18. Serum CCL19 measurement is a new hallmark of the B cell-mediated rheumatoid arthritis subtype and may predict clinical response to rituximab. PMID: 23740460
    19. A high expression of CCL19 was a good prognostic factor of lung adenocarcinoma. PMID: 23922113
    20. Our findings would suggest that CCL19 and CCL21 may not be associated with cervical lymph node metastasis or other clinical and microscopic factors in oral squamous cell carcinoma. PMID: 22976543
    21. a novel signaling pathway that controls EDG-1 up-regulation following stimulation of T cells by CCR7 and CCL19. PMID: 22334704
    22. involvement of CCL19 in AIDS patients with advanced immunodeficiency, potentially mediating both adaptive and maladaptive responses PMID: 22288592
    23. These results suggest a new combinatorial guiding mechanism by CCL19 and CCL21 for the migration and trafficking of CCR7 expressing leukocytes. PMID: 21464944
    24. increased expression of MIP-3alpha favors recruitment of immature DCs to the tumour bed, whereas de novo local expression of SLC and MIP-3beta induces accumulation of mature DCs at the tumour margin forming clusters with T-cells PMID: 20969772
    25. Klebsiella pneumoniae-triggered DC recruit human NK cells in a CCR5-dependent manner leading to increased CCL19-responsiveness and activation of NK cells. PMID: 20865789
    26. Nuclear localization of four and a half LIM domains 2 (FHL2) transgene is lost in mature but not immature bone marrow-derived dendritic cells following stimulation with CCL19. PMID: 20592280
    27. The CCL19 is known to be critically involved in lymphocyte and dendritic cell trafficking, CCL19-binding competition by CRAM might be involved in modulating these processes. PMID: 20002784
    28. CC chemokine CCL19 attracts mature T cells out of the fetal thymus organ culture. PMID: 11869682
    29. Desensitizing concentrations of CCL21 and CCL19 had no significant effects on T lymphocyte transendothelial migration. PMID: 11870628
    30. located in high endothelial venules in an appropriate location to induce transendothelial migration of CLL cells into lymph nodes PMID: 11929789
    31. Characterization of mouse CCX-CKR, a receptor for the lymphocyte-attracting chemokines TECK/mCCL25, SLC/mCCL21 and MIP-3beta/mCCL19: comparison to human CCX-CKR. (CCX-CKR) PMID: 11981810
    32. migration to CCL19 and CCL21 is dependent on phospholipase C and intracellular calcium flux but not on phosphatidylinositol-3 kinase PMID: 14592837
    33. CCL19 and CXCL12 have roles in chemotaxis of mantle cell lymphoma B cells PMID: 14871974
    34. MIP-3beta was expressed in the human endometrium, but our results could not strongly support the hypothesis that MIP-3beta is a potential chemoattractant for endometrial NK cells. PMID: 15019823
    35. The ccl19 migrated poorly towards the GFP UCB or normal (NL) BM CD34+ cells, p210 UCB and CML CD34+ cells. PMID: 15674360
    36. By conditioning naive T cells into a motile dendritic cell-scanning state, CCL19 promotes antigen-independent responses and increases the probability of cognate histocompatibility complex (MHC)-peptide encounter. PMID: 16081805
    37. Nitric oxide (NO) donor NOR4 provides a signal allowing LPS-stimulated DCs to migrate toward CCL19. PMID: 16249377
    38. The maturation, in vitro migration and cytokine production of human DC after stimulation with live H. pylori is reported. PMID: 16500130
    39. The CCL19,CCL21/CCR7 chemokine system is expressed in inflamed muscles of polymyositis and may be involved in the pathomechanism of polymyositis. PMID: 16887149
    40. Despite sharing only 25% sequence identity with CCL21, the amino terminal hexapeptide of CCL19 is capable of performing an in vitro role similar to that of CCL21, resulting in G protein activation of the CCR7 receptor. PMID: 16904643
    41. CXCL13 and CCL19 cooperatively induce significant resistance to TNF-alpha-mediated apoptosis in acute and chronic B cell leukemia CD23+CD5+ B cells, but not in the cells from cord blood. PMID: 17082584
    42. Expression of the CCL19 gene is regulated by a combined action of several members of the NF-kappaB, IRF, and STAT family transcription factors. PMID: 17182562
    43. The abundance of CD83+ plasmacytoid dendritic cells (DCs) in perivascular areas and the overexpression of CCL19 and CCL21 in perivascular cellular foci suggest plasmacytoid DCs are central to the muscle inflammation in juvenile dermatomyositis. PMID: 17469160
    44. These findings suggest hitherto unknown roles of SMAD transcription factors and of CCL19 in the elicitation phase of allergic contact dermatitis. PMID: 17597826
    45. CCL19 plus CXCL13 regulate interaction between B-ALL CD23+CD5+ B cells and CD8+ T cells by inducing activation of PEG10, in turn, resulting in IL-10 overexpression and impairment of tumor-specific cytotoxicity in syngeneic CD8+ T cells. PMID: 17709502
    46. This study suggests that CCL19 plays a role in both the physiological immunosurveillance of the healthy CNS and the pathological maintenance of immune cells in the CNS of MS patients. PMID: 17825430
    47. after HIV-1 infection of CCL19- or CCL21-treated CD4(+) T-cells, low-level HIV-1 production but high concentrations of integrated HIV-1 DNA, approaching that seen in mitogen-stimulated T-cell blasts, was observed PMID: 17881634
    48. CCL19, CCL20 and CCL22 factors could play an additive role in the pathogenesis of the inflammatory process leading to bronchiolar fibro-obliteration in lung transplant patients PMID: 18047937
    49. experiments performed to investigate the survival, adhesion, and metalloproteases secretion indicate that CXCL13 combined with CCL19 and CCL21 mainly affects the chemotaxis of Sezary syndrome cells PMID: 18757429
    50. Complement C1q chemoattracts human dendritic cells and enhances migration of mature dendritic cells to CCL19 via activation of AKT and MAPK pathways.( PMID: 18838169

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 组织特异性：
    Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach.
  • 数据库链接：

    HGNC: 10617

    OMIM: 602227

    KEGG: hsa:6363

    STRING: 9606.ENSP00000308815

    UniGene: Hs.50002