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  4. CASP9 Recombinant Monoclonal Antibody

CASP9 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA004555A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: HL-60 whole cell lysate, LO2 whole cell lysate
      All lanes: CASP9 antibody at 1.8μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 47, 31, 18, 37 KDa
      Observed band size: 47 KDa
    • Immunofluorescence staining of HepG2 cells with CSB-RA004555A0HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
    • Overlay histogram showing K562 cells stained with CSB-RA004555A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    The CASP9 antibody is a recombinant monoclonal antibody matched isotype control by rabbit IgG. Its production process includes the cloning of the human CASP9 DNA gene into the vector and the transfection of the clones into the cell line for in vitro expression. This CASP9 antibody can recognize human CASP9 protein. It has been purified using affinity-chromatography and been tested for use in ELISA, WB, IF, and FC applications.

    CASP9 is an initiator caspase critical to the apoptotic pathway found in many tissues. Correct CASP9 function is required for apoptosis, leading to the normal development of the central nervous system (CNS). Dysfunction of CASP9 causes abnormal tissue development, thus leading to aberrant function, diseases and premature death.

  • Uniprot No.：
    P55211
  • 基因名：
  • 别名：
    APAF-3 antibody; Apoptotic protease Mch-6 antibody; Apoptotic protease-activating factor 3 antibody; CASP-9 antibody; CASP9 antibody; CASP9_HUMAN antibody; Caspase 9 antibody; Caspase-9 subunit p10 antibody; ICE-LAP6 antibody; ICE-like apoptotic protease 6 antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human CASP9
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    2D5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:20-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • 基因功能参考文献：
    1. CASP9 mutations cause recurrent folate-resistant neural tube defects. PMID: 29358613
    2. Study described rare mutations of the apoptosis gene CASP9 identified in neural tube defect cases and demonstrated that the p.Y251C variant impairs the protein's apoptotic function, suggesting it is a loss-of-function variation; also showed that the p.R191G variant inhibited apoptosis under folate-deficient condition, highlighting the effect of gene-environment interactions in this complex disease. PMID: 29365368
    3. these results revealed that caspase9 and activated caspase3 predominantly regulates cell apoptosis in human dental pulp stem cells from deciduous teeth. PMID: 29845240
    4. Low CASP9 expression is associated with Colorectal Cancer. PMID: 29801534
    5. the results of the present study suggest that miR-96-5p, which is frequently upregulated in hepatocellular carcinoma (HCC), inhibits apoptosis by targeting CASP9. Therefore, miR-96-5p may be a potential therapeutic target for HCC PMID: 29658604
    6. CASP9 germline mutations may have played a role at least in part to the susceptibility of development of gliomas in a Li-Fraumeni-like family lacking a TP53 germline mutation. PMID: 27935156
    7. The caspase 9 level was significantly lower and related with oxidant status in patients with polycystic ovary syndrome, while the circulating levels of caspases 3 and 7 were statistically similar in both PCOS and control groups. PMID: 27899026
    8. Results indicate that the apoptotic protease-activating factor 1 (Apaf-1) apoptosome activates caspase-9 in part through sequestration of the inhibitory caspase recruitment domains (CARDs) domain. PMID: 28143931
    9. DES1 plays a key role in palmitic acid-mediated caspase 9 and caspase 3 activation. PMID: 27364952
    10. CASP9 expression associated with inhibition of miR-182. PMID: 28298075
    11. CASP-9 polymorphism is associated with Primary Brain Tumors. PMID: 28870924
    12. High CASP9 expression is associated with Lung Tumorigenesis. PMID: 27197231
    13. knockdown of HMGI-C led to the significant induction of apoptosis via mitochondrial pathway by inducing miR34a and cell cycle arrest in MDA-MB-468 cells in vitro. PMID: 27245202
    14. Results suggest that the formation of the apoptosome accompanied by the activation of caspase-9 may occur in brains affected by multiple system atrophy (MSA), and that a mitochondria-dependent apoptotic pathway may be partially associated with the pathogenesis of MSA. PMID: 27345387
    15. Using recombinant proteins, this study investigated the influence of survivin on the inhibition of caspase-9 by XIAP in vitro. With a fluorescence-based assay for the apoptosome-stimulated activity of caspase-9, the study shows that survivin has no effect on the inhibition of caspase-9 by XIAP, neither in the presence nor in the absence of Smac. PMID: 27865841
    16. Data show that caspase 9 (CASP9) single nucleotide polymorphism (rs1052576) TT genotype was seemed to be associated with higher risk of pathological stage. PMID: 28358701
    17. The content of caspase 9 gene transcripts in peripheral blood leukocytes and plasma level of TNF-alpha were significantly higher in healthy subjects carrying C allele than in carriers of GG genotype. PMID: 28091912
    18. Results show that mRNA and protein levels of HAX-1 in prostate cancer cell lines were significantly higher and inhibits cell apoptosis through caspase-9 inactivation. PMID: 26323553
    19. Renal CASP9 expression is increased in diabetes and increased as diabetic nephropathy progressed. PMID: 27141571
    20. Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. PMID: 26290316
    21. Levels of caspase-9, caspase-10, MAVS, and pIRF7 in mononuclear cells and the disease activity index (SLEDAI) in the systemic lupus erythematosus patients were determined. PMID: 25370148
    22. Expression of mutant caspase-9 correlated with a downregulation of BAFFR (B-cell-activating factor belonging to the TNF family (BAFF) receptor) in B cells and ICOS (inducible T-cell costimulator) in T cells. PMID: 25569260
    23. caspase-9 mediates Puma activation to determine the threshold for overcoming chemoresistance in cancer cells. PMID: 25356864
    24. we suggest that phosphorylation of caspase-9 may be a useful tool to assess the state of gastrointestinal cancer and the effects of anti-cancer therapy. PMID: 25031754
    25. DNA fragmentation, DNA damage, caspase-9 activation and a large increase in the sub-G1 and S cell cycle phases confirmed the occurrence of apoptosis in a time-dependent manner. PMID: 24377517
    26. Data indicate a significant association of two single nucleotide polymorphism (SNPs) in aspase9 (CASP9) and two haplotypes of the four SNP combinations with acute myeloid leukemia (AML) susceptibility. PMID: 24879622
    27. silica and double-stranded RNA (dsRNA) synergistically induces caspase-9-dependent apoptosis, but not inflammasome activation, of bronchial epithelial cells PMID: 24661197
    28. that KAT5 RNAi may result in cleaved casp9 upregulation through p38MAPK activation in Gallbladder carcinoma cells PMID: 24427328
    29. overexpression of iASPP and the low expression of caspase-9 in esophageal cancer are closely correlated with tumor invasion and metastasis PMID: 24405603
    30. the Atg7.caspase-9 complex performs a dual function of linking caspase-9 to the autophagic process while keeping in check its apoptotic activity. PMID: 24362031
    31. The inhibitory effect of Ab42 on the apoptotic pathway is associated with its interaction with procaspase-9 and consequent inhibition of Apaf-1 apoptosome assembly PMID: 24424093
    32. The rs4645981 T allele and the rs4645981 T allele carrier might increase the risk of cancer, but the rs1052576 A allele, rs1052576 A carrier, rs2308941 T allele, and rs2308941 T carrier might be protective. PMID: 23479167
    33. Interactions of caspase-9 with the BIR3 (baculovirus inhibitory repeat 3) exosite are essential for high-affinity binding. PMID: 23203690
    34. In oligozoospermia, spermatogonia presented significant increased active caspase-9 PMID: 23359247
    35. The change of caspase-9 expression from colon mucosa, adenoma to cancer suggested it may be involved in the carcinogenesis of colon cancer. PMID: 24592539
    36. dCas9 effectors can exert positive or negative regulation on the expression of developmentally relevant genes, which can influence cell differentiation status when impinging on a key node in the regulatory network that governs the cell state. PMID: 24346702
    37. BIRC5-31CC and CASP9+83CT/TT genotypes were associated with an increased risk for renal cell carcinoma development in the female group of our southern European study population. PMID: 23645041
    38. OSU-03012 induces apoptosis in human esophageal carcinoma cells through a p53/Bax/cytochrome c/caspase-9-dependent pathway. PMID: 23652278
    39. EGCG, both alone and in combination with cisplatin, promoted the expression of the pro-apoptotic splice isoform of caspase 9 PMID: 23615977
    40. The results demonstrated that 50 microg/mL beta-glucan significantly repressed the expression of the ERCC5 gene, no change in CASP9 expression, and induction of the CYP1A1 gene. PMID: 23424205
    41. CASP-9 polymorphisms are associated with susceptibility to low back pain during military training in Chinese soldiers. PMID: 23725396
    42. Results found that polymorphisms of CASP9 and CASP10 genes may not contribute to CRC risk in Chinese population. PMID: 23303631
    43. Proteolytic processing of the caspase-9 zymogen is required for apoptosome-mediated activation of caspase-9 PMID: 23572523
    44. Polymorphism in Caspase 9 (-1263 A>G) was observed to be associated with susceptibility of papillary thyroid carcinoma (PTC). PMID: 22120515
    45. Induction of caspase-9b expression is due to activation of hnRNP L via phosphorylation to compete/inhibit hnRNP U association with exon 3 of Casp9 mRNA. PMID: 23396972
    46. These results clearly indicated that olive oil phenolic extract and gallic acid were able to inhibit the caspase 9 dependent apoptosis pathway in HeLa cells. PMID: 22086301
    47. The overexpression of c-Jun, p73 and Casp-9 in thymic epithelial tumors is closely related with the pathogenesis and biological behavior of the neoplasms. PMID: 22974165
    48. Data suggest that CD95L-triggered endogenous ceramide increase in Jurkat leukemia T cells is likely involved in the pro-apoptotic mitochondrial pathway leading to caspase-9 activation. PMID: 22306364
    49. caspase-9 is the most important regulator in DLD-1, HCT-116, and HeLa cells PMID: 23038270
    50. CASP9 promoter polymorphisms rs4645978 and rs4645981 are associated with breast cancer susceptibility; CASP9 transcriptional regulation is an important factor during breast cancer development PMID: 22981751

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  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • 数据库链接：

    HGNC: 1511

    OMIM: 602234

    KEGG: hsa:842

    STRING: 9606.ENSP00000330237

    UniGene: Hs.329502