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  4. BIRC5 Recombinant Monoclonal Antibody

BIRC5 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA209144A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA209144A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
  • 其他：

产品详情

  • 产品描述：

    BIRC5 is an immune-related gene that inhibits apoptosis and promotes cell proliferation. As a mitotic checkpoint gene, BIRC5 serves a pivotal role in the onset and development of various types of malignant tumors. BIRC5 is highly expressed in most tumors and leads to poor prognosis in cancer patients. Overexpression of BIRC5 in cancer may inhibit this apoptotic checkpoint and favor aberrant mitosis of transformed cells. BIRC5 can inhibit the migration and invasion of tumor cells, and regulate the expression of angiogenesis-associated factors.

    The production of this recombinant BIRC5 antibody was carried out in vitro. It began with immunization of animals so that the B cells could be obtained. The next step was selection of B cells. The positive cells would be screened out for the next step, single B cell antibody sequencing and cloning. Once the BIRC5 antibody sequence was obtained, it would be inserted into a plasmid, which could be transfected into mammalian cells for the expression of BIRC5 antibody.

  • Uniprot No.：
    O15392
  • 基因名：
  • 别名：
    Baculoviral IAP repeat-containing protein 5 (Apoptosis inhibitor 4) (Apoptosis inhibitor survivin), BIRC5, API4 IAP4
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human Survivin
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    6G11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Essential for the maintenance of mitochondrial integrity and function. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • 基因功能参考文献：
    1. NF-kappaB activation may infer resistance to apoptosis through the expression of anti-apoptotic genes such as Survivin, which showed progressive increase in expression throughout the oesophageal metaplasia-dysplasia-adenocarcinoma sequence. PMID: 29473241
    2. Study demonstrate that ZIC1 plays a tumor suppressive role in breast cancer, by targeting surviving, significantly downregulating its expression. PMID: 29956756
    3. Studying survivin expression in leukocytes of 144 female rheumatoid arthritis patients this study observed that smoking patients had higher survivin transcription and a remarkable spreading of survivin isoforms. PMID: 27915033
    4. Our findings identify survivin as a target of HO-1 and a mediator of adipocyte-induced survival in the metastatic niche. PMID: 29311669
    5. High BIRC5 expression is associated with gefitinib resistance in lung cancer. PMID: 30106446
    6. miR-203 expression also inhibited primary tumor growth in ovaries and metastatic tumors in multiple peritoneal organs including liver and spleen. miR-203 inhibits ovarian tumor metastasis by targeting BIRC5/survivin and attenuating the TGFbeta pathway. PMID: 30241553
    7. Survivin may be implicated in the bcl-2 and p53 pathways and therefore in the biology of PDAC. Its potential use as a survival predictor and therapeutic target represent a promising field. PMID: 30249893
    8. Study in hepatocellular carcinoma cell line elicited a new mechanism in which IGF-1 induced epithelial-mesenchymal transition through regulation of survivin and a downstream pathway. PMID: 29989646
    9. In the present study, liposomeplasmid DNA encoding mutant survivinT34A could inhibit tumor growth of cervical cancer. This inhibition may be associated with an increase in the apoptosis rate of tumor cells and a reduction in angiogenesis. PMID: 29767242
    10. simvastatin significantly inhibited the proliferation and invasion of SACC83 cells, induced apoptosis, and reduced the expression of survivin, which suggests that simvastatin may be a novel target for salivary gland adenoid cystic carcinoma therapy. PMID: 29956779
    11. Survivin is significantly up-regulated in hepatocellular carcinoma (HCC)tissues and associated with tumor growth and lymph node metastasis. Clinical detection of survivin level combined with MRI examination might be beneficial for clinical diagnosis and treatment of HCC PMID: 30010107
    12. Our study identified the STAT3 rs1053004 C/C as a high-risk genotype in MA with lower survivin and VEGF transcription levels in the peripheral blood. PMID: 30226700
    13. Overexpression of miR-485-5p suppresses breast cancer progression and enhances chemosensitivity. Further study demonstrated that miR-485-5p directly targeted the 3'-untranslated region of survivin and overexpression of survivin overcomes the miR-485-5p induced effects on breast cancer. PMID: 29678577
    14. Survivin expression in gastric cancer cells is regulated by DEC1. PMID: 29204860
    15. High serum survivin levels with GG genotype are associated with Brain Tumors. PMID: 30275230
    16. LNC473 could recruit deubiquitinase USP9X to inhibit the ubiquitination level of survivin and then increase survivin expression. PMID: 29605299
    17. Oct4 plays a vital role in the malignant progression of HCC cells through the survivin/STAT3 signaling pathway. PMID: 29901157
    18. Our study results may suggest that high serum survivin levels can show 4 times increased risk of cancer in a subject with a high suspicion of cancer. Furthermore, survivin level was not influenced with demographic characteristics of breast, gastric, colorectal, prostate, ovarian cancer, and glioblastome multiforme. PMID: 29893319
    19. These findings collectively suggest that the triple combination of survivin knockdown with ABT-263 and trametinib treatment, may be a potential strategy for the treatment of KRAS-mutant lung adenocarcinoma. Furthermore, our findings indicate that the welldifferentiated type of KRAS-mutant lung tumors depends, at least in part, on TTF1 for growth. PMID: 29658609
    20. Targeting the Cripto-1/TAK-1/NF-kappaB/Survivin pathway may be an effective approach to combat apoptosis resistance in cancer. PMID: 29807222
    21. suggests SIRT1 may serve as a predictor of poor prognosis in esophageal squamous cell carcinoma, and its mediated tumor-promoting role might be associated with the overexpression of EGFR protein in esophageal squamous cell carcinoma PMID: 29625788
    22. CSN5 directly bound survivin and decreased its ubiquitination to enhance the protein stability of survivin. PMID: 29596838
    23. The survivin gene 3' UTR polymorphisms (rs17878624) show that GG genotype provides substantial protection from non-small-cell lung carcinoma. PMID: 29631694
    24. Concomitant high expression of survivin and VEGF-C is closely associated with LNM status of PTC patients, which suggests their cooperation in the metastatic process. PMID: 29578160
    25. In leukoplakia, the expression of survivin associated with that of ki-67 reinforces the assumption that all these lesions are potentially malignant. PMID: 28346726
    26. Study showed for the first time that the suppression of rheumatiod arthritis fibroblast-like synoviocyte was mediated by phosphatase and tensin homolog involving survivin silencing. PMID: 28337018
    27. ERCC1 expression may also inhibit esophageal squamous cell carcinoma cell apoptosis via regulating survivin expression, and ERCC1 and survivin overexpression are independent predictors of prognosis for ESCC patients who receive chemotherapy and/or radiotherapy PMID: 30075571
    28. let-7b targets PLK1 to inhibit hepatocellular carcinoma cell growth and induce their apoptosis by attenuating the PLK1-mediated Survivin phosphorylation PMID: 29913237
    29. Overexpression of Survivin in glioma cells induces chromosomal instability PMID: 29282022
    30. High BIRC5 expression is associated with ovarian cancer. PMID: 29795564
    31. In III-rd trimester of pregnancy parameters of Timp-1 and Survivin - anti-apoptotic substances concentration were similar in maternal and cord blood in both artery and vein. We found no increased activity of selected antiapoptotic factors. PMID: 28509321
    32. Results form study in non-small-cell lung cancer cells showed that SphK2 plays a critical role in doxorubicin-induced resistance by regulating key anti-apoptotic gene, survivin. PMID: 28950390
    33. Survivin overexpression plays a key role in the chemoresistance of ovarian CSCs. PMID: 30061219
    34. Survivin may play an important role in the occurrence and development of laryngeal carcinoma, and its high expression is related to the poor prognosis of patients with laryngeal cancer. (Meta-analysis) PMID: 29270761
    35. treatment of DLD1 cells with tamoxifen , betaestradiol, or a combination of these two drugs, inhibited cell viability and migration, promoted cell apoptosis, and reduced the mRNA and protein expression levels of survivin in a dose and timedependent manner. These results provide novel experimental basis for hormonal adjuvant therapy for the treatment of colorectal cancers PMID: 28849238
    36. Studies indicated that high survivin expression in renal cell carcinoma (RCC) was associated with poor overall survival [Review]. PMID: 27411378
    37. Nuclear accumulation of survivin is associated with proliferative phenotype and was shown to be a worse prognostic marker in breast ductal carcinoma. PMID: 29517199
    38. Knockdown of BIRC5, a member of the inhibitor of apoptosis protein family, using either lentiviral vector based CRISPR/Cas9 nickase gene editing or inhibition of survivin using the small-molecule inhibitor YM155, results in the suppression of epithelial to mesenchymal transition in retinal pigment epithelial cells. PMID: 29522718
    39. By downregulation of Sp1 and survivin at the late phase of treatment. PMID: 28713892
    40. The expressions of survivin and STAT2 are up-regulated in skin lesions of PV patients, and their mRNA expressions are positively correlated. PMID: 29089085
    41. Findings suggest that lysosome-associated transmembrane protein 4B (LAPTM4B), vascular endothelial growth factor (VEGF), and nuclear survivin expression are significantly correlated in breast cancer, which may be predictive of prognosis as well as effective therapeutic targets for anticancer therapies. PMID: 28476037
    42. HIF-2alpha dictates the resistance of human pancreatic cancer cells to TRAIL under normoxic and hypoxic conditions and transcriptionally regulates survivin expression. PMID: 28476028
    43. survivin may have a role in recurrence in rectal cancer patients treated with surgery and postoperative concurrent chemo-radiation therapy PMID: 27391438
    44. Results identified BIRC5 to be significantly upregulated in the lung squamous cell carcinoma tissues of smoking patients and may play an important role in diagnosis and prognosis. PMID: 28949095
    45. After cancer cell fusion, some fused cells avoid the apoptotic crisis partly owing to survivin, and continue to proliferate, a process that contributes to human cancer progression. PMID: 28193315
    46. High survivin expression is associated with lung cancer and colorectal cancer. PMID: 27602754
    47. Data suggest that Ki-67 index and survivin may be useful biomarkers for rectal cancer with preoperative chemoradiotherapy. PMID: 29491110
    48. inhibition of apoptosis targeting survivin might represent an effective strategy for both obesity and cancer therapy. PMID: 28518147
    49. FAT10 promotes tumor proliferation by directly stabilizing Survivin protein in breast cancer cells. PMID: 27806337
    50. nuclear survivin is a prognostic marker for the progression of oral squamous cell carcinomas. PMID: 28384094

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody.
  • 蛋白家族：
    IAP family
  • 组织特异性：
    Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. Ex
  • 数据库链接：

    HGNC: 593

    OMIM: 603352

    KEGG: hsa:332

    UniGene: Hs.744872