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  4. ATM Recombinant Monoclonal Antibody

ATM Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA618770A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, PC3 whole cell lysate, HepG2 whole cell lysate
      All lanes: ATM antibody at 2.05μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 350 KDa
      Observed band size: 350 KDa
    • IHC image of CSB-RA618770A0HU diluted at 1:205 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA618770A0HU diluted at 1:205 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunoprecipitating ATM in PC3 whole cell lysate
      Lane 1: Rabbit control IgG instead of CSB-RA618770A0HU in PC3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-RA618770A0HU (3μg) + PC3 whole cell lysate (500μg)
      Lane 3: PC3 whole cell lysate (20μg)
  • 其他：

产品详情

  • Uniprot No.：
    Q13315
  • 基因名：
  • 别名：
    A-T mutated antibody; A-T mutated homolog antibody; AT mutated antibody; AT1 antibody; ATA antibody; Ataxia telangiectasia mutated antibody; Ataxia telangiectasia mutated gene antibody; Ataxia telangiectasia mutated homolog (human) antibody; Ataxia telangiectasia mutated homolog antibody; ATC antibody; ATD antibody; ATDC antibody; ATE antibody; ATM antibody; ATM serine/threonine kinase antibody; ATM_HUMAN antibody; DKFZp781A0353 antibody; MGC74674 antibody; OTTHUMP00000232981 antibody; Serine protein kinase ATM antibody; Serine-protein kinase ATM antibody; Serine/threonine-protein kinase ATM antibody; Tefu antibody; TEL1 antibody; TEL1; telomere maintenance 1; homolog antibody; TELO1 antibody; Telomere fusion protein antibody
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human ATM
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    4E11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IP 1:200-1:1000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.
  • 基因功能参考文献：
    1. In mitosis ATM forms a complex with the poly(ADP)ribose polymerase Tankyrase 1, the spindle pole protein NuMA1, and breast cancer protein BRCA1, another crucial DDR player. The complex is required for efficient poly(ADP)ribosylation of NuMA1. PMID: 24553124
    2. ATM was a direct target of miR-181a. PMID: 24531888
    3. A review discusses ATM function and the consequences of its loss during chronic lymphocytic leukemia pathogenesis. PMID: 23906020
    4. Data show that serine-922 of TAX1BP2 protein is the phosphorylation site of ataxia telangiectasia mutated (ATM) kinase. PMID: 24240686
    5. Data indicate that ataxia telangiectasia mutated (ATM) protein phosphorylates BRG1 protein at Ser-721. PMID: 24413084
    6. These results indicate that ATM regulates a sub-set of NF-kappaB dependent genes after a genotoxic stress by direct phosphorylation of p65. PMID: 22715377
    7. Data suggest that expression of cytomegalovirus UL76 up-regulates human interleukin-8 (IL8) expression/secretion in response to DNA damage; both UL76 and human ATM have roles in mechanism of IL8 induction during cytomegalovirus infection. PMID: 24068928
    8. Huntington's disease cells presented a delayed nucleo-shuttling of phosphorylated forms of the ATM kinase. PMID: 24277524
    9. ATM silencing induced partial reduction in levels of Skp2, a component of SCF(Skp2) ubiquitin ligase that controls Cdt1 degradation. PMID: 24280901
    10. Heterozygosity for the ATM rs373759 polymorphism may be a potential risk factor for papillary thyroid cancer. PMID: 23925578
    11. cAMP signaling inhibits radiation-induced ATM activation by PKA-dependent activation of PP2A, and this signaling mechanism augments radiation-induced apoptosis by reducing ATM-dependent activation of NF-kappaB in lung cancer cells. PMID: 24568192
    12. Study found that in human oncogene-transformed and cancer cells, ATM suppressed ARF protein levels and activity in a transcription-independent manner. PMID: 23851489
    13. Tumoral loss of ATM protein was detected more often in patients with a family history of pancreatic cancer than in those without PMID: 24486587
    14. An important role of ATM-mediated Mad1 Serine 214 phosphorylation in mitosis. PMID: 24728176
    15. OCT variants ( OCT1, OCT2 and ATM) were significantly associated with elevated baseline and glucose-induced C-peptide levels in polycystic ovary syndrome PMID: 24533710
    16. The findings implicate an important role of variants in the ATM- CHEK2- BRCA1 axis in modification of the genetic predisposition to papillary thyroid carcinoma and its clinical manifestations. PMID: 24599715
    17. Cuc B also triggers ATM-activated p53-14-3-3-sigma pathways. PMID: 24505404
    18. Data indicate that ATM-depletion can sensitize breast cancer cells to PARP inhibition, suggesting a potential in the treatment of breast cancers low in ATM protein expression/activity, such as those arising in mutant ATM heterozygous carriers. PMID: 24252502
    19. ATM levels were significantly down-regulated in oxaliplatin-resistant colorectal cancer cells. PMID: 24145123
    20. ATM-mediated Snail Serine 100 phosphorylation in response to ionizing irradiation plays an important part in the regulation of radiosensitivity. PMID: 23891091
    21. Our study indicates that the expression of Pim kinases is physiologically related to DNA-PKcs and ATM in ECs. PMID: 22282239
    22. At a low reactive oxygen species condition during genotoxic insult, the ATM/sumoylated-IKKgamma interaction induced NFkappaB activation that resisted JNK-mediated apoptosis. PMID: 24457965
    23. genetic association studies in a population of men in Germany: Data suggest that an SNP near ATM (ataxia telangiectasia mutated protein; rs11212617) is associated with coronary artery disease (but not blood glucose level) in the subjects studied. PMID: 24281401
    24. ATM and ATR each contribute to DNA damage response (DDR) activation caused by BKPyV infection. PMID: 22952448
    25. High ATM expression is associated with breast cancer. PMID: 23857602
    26. Reduced protein expression of ATM is associated with breast carcinoma. PMID: 23117476
    27. ATM protein expression is an independent prognostic marker in sporadic breast cancer. PMID: 24285016
    28. ATM mutation and ATM protein loss had characteristics of old age, distal location of tumor, large tumor size, and histologic intestinal type. PMID: 24324828
    29. ATM/ATR pathway plays an important role in tumor recognition. PMID: 24726882
    30. data confirm previous work showing that Lig3 is required to maintain mtDNA integrity and function, and highlight a new function of ATM in regulating DNA Lig3 stability and consequently mtDNA repair PMID: 24342190
    31. ATM mutations either alone or in combination with 11q deletion lead to demonstrable ATM dysfunction in patients with chronic lymphocytic leukemia. PMID: 23585524
    32. The findings of this study suggested that biallelic ATM-inactivating mutations may present as isolated, generalized dystonia. PMID: 23640770
    33. This study therefore sheds light on the mechanisms underlying AZA resistance, and will enable better understanding of AZA resistance in patients undergoing AZA treatment. PMID: 24680865
    34. NKX3.1 and ATM have a functional interaction leading to ATM activation and then NKX3.1 degradation in a tightly regulated DNA damage response specific to prostate epithelial cells. PMID: 23890999
    35. Activation of H2AX and ATM in varicella-zoster virus infected cells is associated with expression of VZV ORF61 and ORF63. PMID: 24606682
    36. IP7, formed by IP6K2, binds CK2 to enhance its phosphorylation of the Tti1/Tel2 complex, thereby stabilizing DNA-PKcs and ATM. This process stimulates p53 phosphorylation at serine 15 to activate the cell death program. PMID: 24657168
    37. ATM and MDC1 maintain genomic stability not only by controlling the DNA damage response, but also by regulating spindle assembly checkpoint activation, providing an important link between these two essential biological processes. PMID: 24509855
    38. we elucidated the prognostic implications of the expressions of ATM, Chk2, and p53, in gastric carcinoma PMID: 23969480
    39. The regulation of ATM by HDAC enzymes therefore suggests a vital role for HDAC1 and HDAC2 in the DNA damage response. PMID: 23939379
    40. Apoptotic progression is markedly attenuated by ATM gene knockdown through downregulation of caspase-8 and caspase-9. PMID: 24530529
    41. ATM and MAPKAP kinase 2 mediate radiation sensitivity in pancreatic cancer cells via phosphorylation of TRIM29. PMID: 24469230
    42. data provide strong evidences that Aurora-A and BRCA1/2 inversely control the sensitivity of cancer cells to radio- and chemotherapy through the ATM/Chk2-mediated DNA repair networks PMID: 24480460
    43. glioma stem cells were more resistant to radiation compared to glioma cells due to high expression of phosphorylated cell cycle checkpoint proteins, and inhibition of ATM could significantly reduce the radioresistance of glioma stem cells and glioma cells PMID: 23846672
    44. ATM-deficient mice show resistance to hepatocyte cell death. PMID: 23435430
    45. mimosine treatment, ATM blocks S phase entry in response to ROS, which prevents replication fork stalling-induced DNA damage. PMID: 24421316
    46. In Rho 0-Hep G2 cells, which lack mitochondrial DNA and functional mitochondria, ATM failed to respond to hydrogen peroxide, indicating that mitochondria are required for the oxidative activation of ATM. PMID: 24406161
    47. 12 pathogenic Atm mutations (1 missense, 4 nonsense, 5 frameshift, 1 splicing, and 1 large genomic deletion) were found in 8 Chinese patients from 5 families. All were novel. No homozygous mutation and founder-effect mutation were found. PMID: 23807571
    48. Heterozygous carriers of c.8851-1G>T (associated with absence of ATM kinase activity) had a stronger radiosensitive phenotype with this assay than heterozygous carriers of p.Asp2708Asn (associated with residual kinase activity). PMID: 23632773
    49. Significant association with PTC was found for rs1801516 (D1853N) in ATM and rs1867277 in the promoter region of FOXE1 (OR = 1.55, 95% CI 1.03, 2.34). PMID: 24105688
    50. Kaposi's sarcoma-associated herpesvirus induces the ATM and H2AX DNA damage response early during de novo infection of primary endothelial cells, which play roles in latency establishment. PMID: 24352470

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  • 相关疾病：
    Ataxia telangiectasia (AT)
  • 亚细胞定位：
    Nucleus. Cytoplasmic vesicle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    PI3/PI4-kinase family, ATM subfamily
  • 组织特异性：
    Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
  • 数据库链接：

    HGNC: 795

    OMIM: 208900

    KEGG: hsa:472

    STRING: 9606.ENSP00000278616

    UniGene: Hs.367437