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  4. AGTR2 Recombinant Monoclonal Antibody

AGTR2 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA944053A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: 293T whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Mouse liver tissue, Mouse heart tissue
      All lanes: AGTR2 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42 kDa
      Observed band size: 42 kDa
  • 其他：

产品详情

  • 产品描述：

    The production of the recombinant AGTR2 antibody depended on Single B Cell technology. There are 3 main steps in the production: 1, Isolation of single B cells. High-throughput methods could be used to obtain the efficient identification and desired specificity of B cells. 10, Single B cell antibody sequencing and cloning. In this step, the antibody gene sequence of AGTR2 was obtained and introduced to plasmids, which then would be transferred to mammalian cells for in vitro expression of the AGTR2 antibody. 3, Screening of antibodies. The target antibody was obtained in this step. And it has been validated in ELISA, WB.

    AGTR2, also called AT2R, is a G-protein coupled receptor. AT2R expression rises in response to damage and pathological remodeling, implying that AT2R plays a role in tissue repair and regeneration. AT2R activation by AngII is thought to directly counter AT1R signaling, resulting in vasodilation and blood pressure reduction. Furthermore, AT2R activation by Ang(1-9) and, potentially by Ang(1-7), exerts protective, antifibrotic, and anti-inflammatory activities in a number of tissues, including the heart, lung, kidney, and brain, according to numerous studies. AT2R can regulate sympathoexcitation in animals with hypertension and heart failure.

  • Uniprot No.：
    P50052
  • 基因名：
  • 别名：
    Type-2 angiotensin II receptor (Angiotensin II type-2 receptor) (AT2), AGTR2
  • 反应种属：
    Human, Mouse
  • 免疫原：
    A synthesized peptide derived from human AGTR2
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    6C7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Receptor for angiotensin II. Cooperates with MTUS1 to inhibit ERK2 activation and cell proliferation.
  • 基因功能参考文献：
    1. Intracrine action of angiotensin II in mesangial cells: subcellular distribution of angiotensin II receptor subtypes AT1 and AT2 PMID: 29455433
    2. rs5193, rs1403543 and rs12710567 of AT2R gene might have no effect on pregnancy-induced hypertension risk among Chinese Han women. PMID: 29714512
    3. It was found that the AGTR2 A-allele is a candidate genetic marker for top-level power athletes. Carriers of the minor allele (A-allele in males and A/A genotype in females) may possess some molecular advantage in developing muscle strength and power traits. PMID: 29561708
    4. Low levels of AT2R is associated with angiogenesis in bladder cancer. PMID: 28599664
    5. angiotensin II type 2 receptor activation in inguinal adipocytes opposes norepinephrine-induced uncoupling protein-1 (UCP1) production and aspects of cellular respiration PMID: 27477281
    6. loss of AT2 R is associated with podocyte loss/dysfunction and is mediated, at least in part, via augmented ectopic hedgehog interacting protein expression in podocytes PMID: 28722118
    7. AGTR2, which encodes angiotensin II receptor type 2, plays a role in modulating uteroplacental circulation and harbors variants that may contribute to the risk of preeclampsia. It is unlikely that the association that we identified with AGTR2 indicates a risk of preeclampsia rather than of spontaneous preterm birth, because women with preeclampsia as a reason for their delivery were excluded from the Nordic studies. PMID: 28877031
    8. In this genomewide association study, we found that variants at the EBF1, EEFSEC, AGTR2, WNT4, ADCY5, and RAP2C loci were associated with gestational duration and variants at the EBF1, EEFSEC, and AGTR2 loci with preterm birth. PMID: 28877031
    9. crystal structures of human AT2R bound to an AT2R-selective ligand and to an AT1R/AT2R dual ligand, capturing the receptor in an active-like conformation PMID: 28379944
    10. Suggest a gender-specific association between the AT2R -1332 A/G polymorphism and the occurrence of carotid plaque and the history of cerebrovascular insult in advanced carotid atherosclerosis. PMID: 27062416
    11. Significant overrepresentation of AT2R-1332 AA genotype in female multiple sclerosis patients was found, compared to female controls. PMID: 27000216
    12. Ang II-induced upregulation of ATF3 and SUMO1 in vitro and in vivo was blocked by Ang II type I receptor antagonist olmesartan. Moreover, Ang II induced ATF3 SUMOylation at lysine 42, which is SUMO1 dependent PMID: 26850942
    13. The A1166C polymorphism of AT1R, A1675G and C3123A polymorphisms of AT2R were analyzed. The A1675G polymorphism of AT2R might be associated with preeclampsia PMID: 25816156
    14. AT2R downregulates the expression of TGF-betaRII in human proximal tubule cells PMID: 26867007
    15. Angiotensin II type 2 receptor is present in human semen and may be involved in the control of sperm motility and male fertility. PMID: 26616438
    16. ATGR2 activation enhances ACE2 expression and activity and prevents TNF-alpha-stimulated ICAM-1 expression . PMID: 26163449
    17. Our results support that the AGTR2 gene may contribute to the pathogenesis of UPJO and the genetic origin of CAKUT could vary according to phenotype expression PMID: 24995698
    18. in colorectal cancer, AT2R expression showed an inverse correlation with tumor stage and liver metastasis PMID: 25138435
    19. Suggest a key regulatory role of ATI and ATII in promoting cellular proliferation in infantile hemangioma, and establish a role for ACE and ATIIR2 in the proliferation of this tumour. PMID: 25713419
    20. The AGTR2 gene C allele is associated with an increased proportion of slow-twitch muscle fibres. PMID: 24887114
    21. Activation of intracellular angiotensin AT receptors induces rapid cell death in non-cycling uterine leiomyosarcoma cells. PMID: 25487516
    22. Type 2 angiotensin II receptor (ATR2) expression was 3.5-fold higher in bronchoalveolar cells 1-6 h after lung with cardiopulmonary bypass transplantation. PMID: 24796630
    23. Loss of angiotensin II type 2 receptor is associated with hepatocellular carcinoma. PMID: 24391821
    24. Ang II induces corneal myofibroblasts resistance to apoptosis via activating NF-kappaB signaling pathway, and thus should be further investigated as a possible target for therapy of corneal fibrosis. PMID: 24917146
    25. These data indicate that the role of AT2R in human diseased arteries is divergent although the AT2R-mediated vasorelaxation prevails. PMID: 24674681
    26. G allele of AT2R - 1332 G:A polymorphism is associated with an increased risk of preeclampsia. PMID: 24440243
    27. Angiotensin II type 2 receptor antagonists could be particularly useful in the treatment of chronic pain and hypersensitivity associated with abnormal nerve sprouting PMID: 23255326
    28. In light of knowledge that AT2 R expression is regulated by oestrogen and that the prevalence of hypertension and cardiovascular risk is greater in women after menopause, AT2 R agonist therapy may represent an approach to treat hypertension. PMID: 23406015
    29. AGTR2 C4599A in mothers, fathers and babies was associated with preeclampsia and this association was only apparent in pregnancies in which the women had a BMI >/= 25 kg/m(2), suggesting a gene-environment interaction. PMID: 23122839
    30. angiotensin II type 2 receptor (AT2R) gene adenine/cytosine (A/C)-3123 polymorphism may be a marker associated with HDL metabolism by hypertension PMID: 22869520
    31. The polymorphism at AT (2) R gene was associated with the risk for aldosterone-producing adenoma. PMID: 21671168
    32. The expression of AT2R in aldosterone-producing adenoma of the adrenal gland was lower than that in normal adrenal gland tissues. AT2R expression was negatively related to plasma aldosterone concentration but positively related with plasma renin activity. PMID: 20714875
    33. Dopamine and angiotensin type 2 receptors cooperatively inhibit sodium transport in human renal proximal tubule cells. PMID: 22710646
    34. In the present study, we showed that the 3123 C/A polymorphism of AGT2R gene is not a significant factor for hypertension in a sample of Tunisian population PMID: 22987376
    35. angiotensin II receptor type 2 receptors are indispensable during islet -like cell clusters maturation in vivo. PMID: 22162314
    36. The data suggested that diameter of the retinal arterioles might be associated with the angiotensin II Type 2 Receptor 1675G/A polymorphism. PMID: 21850060
    37. This study therefore confirms a hypertrophy-modulating effect for AT(2)R also in hypertrophic cardiomyopathy. PMID: 21163866
    38. The present findings suggest that the homozygous form of the ATR gene may make improving systolic blood pressure and some obesity-related metabolic parameters through a dietary intervention difficult among obese women. PMID: 20371166
    39. A1166C genetic variation of the angiotensin II type I receptor gene may not have a major role in susceptibility to coronary heart disease [meta-analysis] PMID: 20732682
    40. ACE DD and AT2R GG genotypes are associated with chronic kidney disease and scarring in PUV PMID: 20149750
    41. Angiotensin II type 2 receptor signaling significantly attenuates growth of murine pancreatic carcinoma grafts in syngeneic mice PMID: 20181281
    42. Variations in AGTR1 and AGTR2 are associated with sudden cardiac arrest risk in a population-based case-control study. PMID: 19716087
    43. The aim of this study was to test the hypothesis that ACE I/D and AT2R 1675G>A are more frequent in patients suffering from the most severe episodes of hypoglycaemia requiring medical emergency treatment. PMID: 19820429
    44. The highest levels of expression of AT2R were found in early and mid TOP placentae compared to term pregnancy. PMID: 20304486
    45. The constitutive and ligand-mediated activity as well as the signal transduction of the AT(2) receptor, focusing on adapter proteins which directly bind to this receptor, is discussed. PMID: 19759061
    46. The loss of AT(2) receptor signalling is associated with increased renal injury and mortality in chronic kidney disease. PMID: 19861345
    47. This review focuses on the role of the AT(2) receptor in the kidney. PMID: 19861347
    48. The role of the AT(2) receptor on cardiovascular structure and disease, and the signalling pathways induced by its activation, is discussed. PMID: 19861349
    49. This review focuses primarily on the common polymorphism -1332G/A of angiotensin II type 2 (AT(2)) receptor but also briefly mentions 3123C/A (located in the 3'-UTR). PMID: 19861351
    50. Novel data from human oesophagus and jejunum suggest that the AT(1) receptor mediates muscular contractions and that the AT(2) receptor regulates epithelial functions. PMID: 19861352

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    In adult, highly expressed in myometrium with lower levels in adrenal gland and fallopian tube. Expressed in the cerebellum. Very highly expressed in fetal kidney and intestine.
  • 数据库链接：

    HGNC: 338

    OMIM: 300034

    KEGG: hsa:186

    STRING: 9606.ENSP00000360973

    UniGene: Hs.405348