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  4. USP14 Recombinant Monoclonal Antibody

USP14 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA587609A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: PC3 whole cell lysate, Ntera-2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate
      All lanes: USP14 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 57, 53, 55 kDa
      Observed band size: 55-72 kDa
    • Immunofluorescence staining of PC-3 cell with CSB-RA587609A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 517-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • Uniprot No.：
    P54578
  • 基因名：
    USP14
  • 别名：
    Ubiquitin carboxyl-terminal hydrolase 14 (EC 3.4.19.12) (Deubiquitinating enzyme 14) (Ubiquitin thioesterase 14) (Ubiquitin-specific-processing protease 14), USP14, TGT
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human USP14
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    28C4
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation.
  • 基因功能参考文献：
    1. By displacing USP14, TRIM11 changes proteasome composition and suppresses both catalytic and non-catalytic effects of USP14 PMID: 29581427
    2. Usp14 and Ube3c cycle together on and off proteasomes, and the presence of ubiquitinated substrates promotes their association. This mechanism enables proteasome activity to adapt to the supply of substrates. PMID: 28396413
    3. Study detected elevated USP14 expression at both mRNA and protein levels, and was significantly associated with distant metastasis. PMID: 28509417
    4. These findings suggested that USP14 induces NF-kappaB activity and ERK1/2 phosphorylation triggered by microbial infection. PMID: 28364380
    5. Ubiquitin-specific protease 14 regulates cell proliferation and apoptosis in oral squamous cell carcinoma PMID: 27592452
    6. our report demonstrates significant value in targeting USP14/UCHL5 with VLX1570 in drug-resistant Waldenstrom macroglobulinemia (WM) and carries a high potential for clinical translation PMID: 27813535
    7. USP14 promotes prostate cancer progression. PMID: 28151478
    8. without a ubiquitinated substrate present, Usp14 suppresses multiple proteasomal activities, especially basal ATP consumption and degradation of non-ubiquitinated proteins. These allosteric effects thus reduce ATP hydrolysis by inactive proteasomes and nonspecific proteolysis and enhance proteasomal specificity for ubiquitinated proteins. PMID: 28416611
    9. USP14 regulates autophagy by negatively controlling K63 ubiquitination of Beclin 1 PMID: 27542828
    10. USP14 plays an important role in the progression and metastasis of esophageal squamous cell carcinoma. PMID: 27629392
    11. USP14 participates in CAM-DR of MM through acting as a bridge between Bcl-xl apoptotic pathway and Wnt-signaling pathways and may be represented as a good candidate for pursuing clinical trials in MM. PMID: 26710889
    12. Results show that USP14 mRNA is overexpressed in hepatocellular carcinoma (HCC) tissues and inversely correlates with miR-4782-3p level which binds to USP14 3'UTR to regulate its expression. PMID: 26782643
    13. Results found USP14 amplification and overexpression in many different cancers. Its overexpression in low-expression cell lines promoted cell proliferation and migration, whereas its downregulation suppressed tumor cell proliferation, increased apoptosis rate, and decreased cell migration and invasion suggesting an oncogenic role in various types of cancer. PMID: 26938858
    14. Increased USP14 expression in patients with breast cancer was associated with a poorer prognosis. PMID: 26712154
    15. Akt-mediated phosphorylation of USP14 at Ser432, which normally blocks its catalytic site in the inactive conformation, activates its deubiquitinating activity in vitro and in cells. PMID: 26523394
    16. our findings suggested that USP14 is involved in the progression of hepatocellular carcinoma(HCC) and may be a useful therapeutic target in HCC PMID: 26397990
    17. Cell surface ubiquitination precedes endocytosis, after which USP14 acts as an ubiquitin-binding protein that targets the ubiquitinated GABA B receptor to lysosomal degradation and promotes its deubiquitination. PMID: 26817839
    18. USP14 shows a marked preference for ubiquitin-cyclin B conjugates that carry more than one ubiquitin modification or chain PMID: 27074503
    19. our findings suggest that USP14 is involved in the progression of epithelial ovarian cancer PMID: 25429837
    20. Downregulation of USP14 expression arrested the cell cycle, which may be related to beta-catenin degradation. PMID: 23702845
    21. b-AP15 is an inhibitor of deubiquitylating enzyme USP14 and UCHL5 induces apoptosis in multiple myeloma and overcomes bortezomib resistance. PMID: 24319254
    22. overexpression of HA-USP14 increased the LPS-, TNFalpha-, or Escherichia coli-induced IL-8 release in human lung epithelial cells. PMID: 23615914
    23. the decrease in proteolysis of proteasomal substrates during aging is independent of the increased USP14 activity and that the reciprocal regulation of USP14 and proteasomal catalytic activity may be necessary to maintain cellular ubiquitin homeostasis. PMID: 23291607
    24. High Ubiquitin-specific protease 14 expression associated with intrahepatic cholangiocarcinoma cell differentiation. PMID: 21627382
    25. the catalytic cleft leading to the active site of USP14 is blocked by two surface loops. Binding by ubiquitin induces a significant conformational change thereby allowing access of the ubiquitin C-terminus to the active site. PMID: 16211010
    26. Results show the overall survival rate was worse in patients with a high USP14/TGT60 kD expression level and suggest that USP14/TGT60 kD also controls the fate of proteins that regulate tumor invasion and metastasis. PMID: 16465409
    27. Neuronal expression of full-length USP14 is able to restore the levels of monomeric ubiquitin in the brains of transgenic ataxia mice. PMID: 17079671
    28. USP14 were over-expressed in ovarian serous cystadenocarcinoma tissues, suggesting that the activity of ubiquitin-proteasome system is obviously enhanced in ovarian cancer. PMID: 17553343
    29. These findings suggest that USP14 is a novel player in the unfolded protein response by serving as a physiological inhibitor of ER-associated degradation under the non-stressed condition. PMID: 19135427
    30. Data from transgenic mice define a critical role for Usp14 at mammalian synapses and suggest a requirement for local ubiquitin recycling by the proteasome to control the development and function of neuromuscular junctions. PMID: 19726649

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Peptidase C19 family, USP14/UBP6 subfamily
  • 数据库链接：

    HGNC: 12612

    OMIM: 607274

    KEGG: hsa:9097

    STRING: 9606.ENSP00000261601

    UniGene: Hs.464416