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  4. STK3 Recombinant Monoclonal Antibody

STK3 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA980100A0HU
  • 规格：
    ￥1320
  • 图片：
    • Western Blot
      Positive WB detected in: U251 whole cell lysate, U87 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate
      All lanes: STK3 antibody at 1:2000
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 57, 60 kDa
      Observed band size: 50-70 kDa
  • 其他：

产品详情

  • Uniprot No.：
    Q13188
  • 基因名：
  • 别名：
    Serine/threonine-protein kinase 3 (EC 2.7.11.1) (Mammalian STE20-like protein kinase 2) (MST-2) (STE20-like kinase MST2) (Serine/threonine-protein kinase Krs-1) [Cleaved into: Serine/threonine-protein kinase 3 36kDa subunit (MST2/N), Serine/threonine-protein kinase 3 20kDa subunit (MST2/C)], STK3, KRS1 MST2
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human STK3
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    3C3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1. Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.
  • 基因功能参考文献：
    1. These results suggest Salvador enhances the effects of Hippo kinase activity at multiple points in the Hippo pathway. PMID: 29519817
    2. Integration analysis of esophageal squamous cell carcinoma (ESCC) identified five hyper-methylated CpG sites in STK3, ZNF418 and ZNF542 that can be used for effective methylation-based testing for ESCC diagnosis. PMID: 29270239
    3. High MST2 expression is associated with malignant melanoma. PMID: 28677804
    4. Here, the s discover SAV1-mediated inhibition of the PP2A complex STRIPAK(SLMAP) as a key mechanism of MST1/2 activation. PMID: 29063833
    5. Through a comprehensive set of biochemical, biophysical, mutational and structural studies, we quantitatively assess how phosphorylation of MOB1A regulates its interaction with both MST kinases and LATS/NDR family kinases in vitro. PMID: 28373297
    6. In this study, we investigated the mechanisms behind the recruitment of MST1 and MST2 kinases to MOB1, which facilitate signal transmission in the Hippo pathway by bringing the MST1 and MST2 kinases in close vicinity to their substrates, the LATS family kinases. PMID: 28373298
    7. These findings reveal a novel layer of regulation for MST2 in mitosis and its role in tumorigenesis. PMID: 27566175
    8. TNFAIP8 regulates Hippo (MST1/2) signaling through its interaction with LATS1. PMID: 28152516
    9. Data suggest that Hippo (MST1/2 protein kinases) - Yes associated protein 1 (YAP) pathway involved in carcinogenesis of pancreatic cancer and in the inhibition effect of stiehopus japonieus acidic mucopolysaccharide (SJAMP) to the proliferation of pancreatic cancer cell. PMID: 28099921
    10. H-ras, via an Erk-dependent mechanism, downregulates Mst1/Mst2 activity by inducing the formation of inactive Mst1/Mst2 heterodimers. PMID: 27238285
    11. Results show that STK3 is targeted by Casp6 and demonstrate that alterations in STK3 protein expression levels and post-translational modifications are detected in a cellular model of HD and caspase-mediated generation of STK3 fragments observed under conditions of stress in cells expressing mhtt. PMID: 26908611
    12. using an Mst2 mutation that disrupts homotypic dimerization, we showed that the monomeric Mst2-SARAH domain could form a stable complex of 1:1 stoichiometric ratio with WW45 refolded under acidic pH. PMID: 25814670
    13. The MST1/2-SAV1 complex, a core component of the Hippo pathway, promotes ciliogenesis. PMID: 25367221
    14. These results suggest that AIF downregulation is a common event in kidney tumor development. AIF loss may lead to decreased STK3 activity, defective apoptosis and malignant transformation PMID: 24992339
    15. Hippo and Yap regulate cardiomyocyte death and regeneration. PMID: 24881775
    16. results reveal a novel role of Mst2 in stress-dependent cardiac hypertrophy and remodeling in the adult mouse and likely human heart PMID: 25035424
    17. Results indicate that changes in phosphorylation orchestrate interactions between kinases and phosphatases in Hippo (MST1/2 protein kinases) signaling, providing a putative mechanism for pathway regulation. PMID: 24255178
    18. RASSF5 can act as an inhibitor or a potential positive regulator of Mst2, depending on whether it binds to Mst2 before or after activation-loop phosphorylation. PMID: 23972470
    19. results of the present study revealed that, in addition to the phosphorylated YAP/TAZ, the Hippo pathway can suppress the Wnt/beta-catenin pathway directly through MST1/2 PMID: 24180524
    20. The ability of K-Ras to activate MST2 and MST2-dependent apoptosis is determined by the differential activation kinetics of mutant K-Ras and wild type K-Ras. PMID: 23459937
    21. The identification of the c-Abl tyrosine kinase as a novel upstream activator of MST2 suggests that the conserved c-Abl-MST signaling cascade plays an important role in oxidative stress-induced neuronal cell death. PMID: 22590567
    22. crystals of MST2 belonged to space group P2, with unit-cell parameters a = 62.0, b = 119.2, c = 62.0 A, alpha = 90.0, beta = 90.5, gamma = 90.0 degrees , or to space group P6(1)22, with unit-cell parameters a = 54.5, b = 54.5, c = 303.1 A PMID: 22102242
    23. The small number of tumors with co-expression of mutant K-Ras and MST2 has elevated apoptosis rates. PMID: 22195963
    24. The mammalian ste20-like kinase mediated phosphorylation of four residues within SAV1 may be important in the induction of cell death by the MST pathway. PMID: 21944251
    25. MST and hSAV act as novel co-regulators of ERalpha and may play an important role in breast cancer pathogenesis. PMID: 21104395
    26. Through binding to MST1/2, RASSF1A supports maintenance of MST1/2 phosphorylation, promoting an active state of the MST kinases and favoring induction of apoptosis. PMID: 21199877
    27. ste20-like kinase MST2 is regulated by the IGF1-Akt pathway PMID: 20231902
    28. Study reports that two Hippo pathway components, Mst2 and the scaffold protein hSav1, directly interact with Nek2A and regulate its ability to localize to centrosomes, and phosphorylate C-Nap1 and rootletin. PMID: 21076410
    29. studies reveal a more complex role for Mst2 than previously thought. The Mst2 --> LATS1/2 pathway, by maintaining PP2A-C levels, may, in some situations, positively affect mitogenic signaling PMID: 20212043
    30. hnRNP H blocks MST2-mediated apoptosis in cancer cells by regulating A-Raf transcription. PMID: 20145135
    31. Data show T117/T384 as Akt phosphorylation sites in MST2, and mutation of these sites inhibited MST2 binding to Raf-1 but enhanced binding to RASSF1A, accentuating downstream c-jun N-Terminal Kinase and p38 MAPK signaling and promoting apoptosis. PMID: 20086174
    32. Caspase-catalyzed cleavage and activation of Mst2 correlates with eosinophil but not neutrophil apoptosis. PMID: 11964314
    33. novel mechanism for MST2 regulation in apoptotic cells postulated; during apoptosis MST2 is cleaved by caspase-3 and undergoes irreversible autophosphorylation resulting in the accumulation of active MST2 PMID: 12554736
    34. proteomic analysis of Raf-1 signaling complexes was used to show that Raf-1 counteracts apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2) PMID: 15618521
    35. Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway. PMID: 15688006
    36. our results suggest that alteration of the Sav-RASSF1-Hpo tumor suppressor pathway may occur through hypermethylation of the CpG island promoter of MST1, MST2 and/or RASSF1A in human sarcomas. PMID: 17538946
    37. These results describe an MST2-dependent effector pathway for RASSF1A proapoptotic signaling and indicate that silencing of RASSF1A in tumors removes a proapoptotic signal emanating from p73. PMID: 17889669
    38. phosphorylation of MOB1 at Thr74 by MST2 is essential to make a complex of MOB1, MST2 and NDR1, and to fully activate NDR1 PMID: 18362890
    39. Overexpression of YAP2 in cells promoted apoptosis, whereas the Mst2/Lats1-induced phosphorylation of YAP partially rescued the cells from apoptotic death. PMID: 18640976
    40. Results suggest that MST2-, Fry-, and MOB2-mediated activation of NDR1 is crucial for the fidelity of mitotic chromosome alignment in mammalian cells. PMID: 19327996
    41. MST2 and RASSF2 form an active complex in vivo, in which RASSF2 is maintained in a phosphorylated state and protects MST2 from degradation and turnover PMID: 19525978
    42. Data suggest thta MST kinases 1/2 serve to monitor cytoskeletal integrity and couple the JNK1/SAPK1 pathway to the regulation of the cell cycle regulatory protein p21Waf1. PMID: 19822666

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
  • 组织特异性：
    Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
  • 数据库链接：

    HGNC: 11406

    OMIM: 605030

    KEGG: hsa:6788

    STRING: 9606.ENSP00000390500

    UniGene: Hs.492333