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PDE2A Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA150831A0HU
  • 规格：
    ￥1320
  • 图片：
    • Immunofluorescence staining of HepG2 cell with CSB-RA178866A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 569-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • 其他：

产品详情

  • Uniprot No.：
    O00408
  • 基因名：
  • 别名：
    cGMP-dependent 3',5'-cyclic phosphodiesterase (EC 3.1.4.17) (Cyclic GMP-stimulated phosphodiesterase) (CGS-PDE) (cGSPDE), PDE2A
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from human PDE2A
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    11C6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IF
  • 推荐稀释比：
    Application Recommended Dilution
    IF 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Plays an important role in growth and invasion of malignant melanoma cells (e.g. pseudomyxoma peritonei (PMP) cell line).; Regulates mitochondrial cAMP levels and respiration. Involved in the regulation of mitochondria morphology/dynamics and apoptotic cell death via local modulation of cAMP/PKA signaling in the mitochondrion, including the monitoring of local cAMP levels at the outer mitochondrial membrane and of PKA-dependent phosphorylation of DNM1L.
  • 基因功能参考文献：
    1. the well-known interaction between AIP and 2 different isoforms of phosphodiesterases (PDEs), PDE2A3 and PDE4A5, is of particular interest. While the interaction with over-expressed AIP does not seem to affect PDE2A3 function, the reported effect on PDE4A5 is, in contrast, reduced enzymatic activity. PMID: 28427099
    2. Lu AF33241 represents a novel PDE2A/PDE10A inhibitor tool which can penetrate the blood brain barrier. PMID: 25941078
    3. Epigenetic regulation of phosphodiesterases 2A and 3A underlies compromised beta-adrenergic Signaling in an induced pluripotent stem cell model of dilated cardiomyopathy. PMID: 26095046
    4. curcumin exerts its in vitro anti-angiogenic and in vivo anti-tumour properties through combined PDE2 and PDE4 inhibition PMID: 25230992
    5. PDE2A is involved in growth and invasion of human malignant melanoma PMP cells. PMID: 24705027
    6. This study reports the X-ray crystal structure of PDE2A in complex with a highly selective, nanomolar inhibitor (BAY60-7550) at 1.9 A resolution, and the structure of apo PDE2 at 2.0 A resolution. PMID: 23899287
    7. PDE2 overexpression in healthy cardiomyocytes reduces the rise in cyclic AMP levels and L-type calcium current amplitude, and abolishes the inotropic effect following acute beta-adrenergic receptor stimulation. PMID: 23810893
    8. Our results demonstrated that the PDE2A2 variant carrying point mutations is expressed in PMP cells and may affect cell cycle progression by modulating cyclin A expression. PMID: 23381931
    9. found in smooth muscle wall of blood vessels transversing the clitoral supepithelial and stromal space PMID: 21697861
    10. Active site similarity between human and Plasmodium falciparum phosphodiesterases: considerations for antimalarial drug design PMID: 21766240
    11. the phosphodiesterase 2A isoform localized to mitochondria regulates respiration PMID: 21724846
    12. Inhibition of PDE2 resulted in higher levels of intracellular cAMP than inhibition of PDE3A suggesting this PDE may be the more important regulator of cAMP in human platelets. PMID: 12038792
    13. TNF-alpha-mediated up-regulation of PDE2 may destabilize endothelial barrier function in sepsis PMID: 15650061
    14. Phosphodiesterase 2 mediates redox-sensitive endothelial cell proliferation and angiogenesis by thrombin via Rac1 and NADPH oxidase 2. PMID: 19390057
    15. report the X-ray crystal structures of a PDE protein that includes both catalytic and regulatory domains, namely, PDE2A containing the N-terminal GAF domains and the catalytic domain. PMID: 19828435

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  • 亚细胞定位：
    [Isoform PDE2A3]: Cell membrane; Lipid-anchor.; [Isoform PDE2A2]: Mitochondrion matrix. Mitochondrion inner membrane. Mitochondrion outer membrane.; [Isoform PDE2A1]: Cytoplasm.; [Isoform 5]: Mitochondrion.
  • 蛋白家族：
    Cyclic nucleotide phosphodiesterase family, PDE2 subfamily
  • 组织特异性：
    Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.
  • 数据库链接：

    HGNC: 8777

    OMIM: 602658

    KEGG: hsa:5138

    STRING: 9606.ENSP00000334910

    UniGene: Hs.503163