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  4. LEF1 Recombinant Monoclonal Antibody

LEF1 Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA561630A0HU
  • 规格：
    ￥1320
  • 图片：
    • IHC image of CSB-RA561630A0HU diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.
    • IHC image of CSB-RA561630A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.52% DAB.
  • 其他：

产品详情

  • Uniprot No.：
    Q9UJU2
  • 基因名：
  • 别名：
    Lymphoid enhancer-binding factor 1 (LEF-1) (T cell-specific transcription factor 1-alpha) (TCF1-alpha), LEF1
  • 反应种属：
    Human
  • 免疫原：
    A synthesized peptide derived from Human LEF1
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Rabbit IgG
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    13B7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, IHC
  • 推荐稀释比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. May play a role in hair cell differentiation and follicle morphogenesis.; Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.; Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling.; Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
  • 基因功能参考文献：
    1. Experimental analysis showed the importance of LEF1, ETV4 and FABP6 as three co-regulated prognostic markers in patients with colorectal cancer metastasis. PMID: 30193961
    2. LEF-1 and CDX2 performed at least as well as beta-catenin, if not better, as a diagnostic marker for pilomatrical carcinomas PMID: 29369390
    3. study identifies a novel mechanism of hepatocellular carcinoma inhibition through beta-catenin-independent Wnt signalling, which is regulated by WT1-associated LEF1 repression. The study also highlights mangiferin as a promising Wnt inhibitor for HCC treatment. PMID: 29953980
    4. High LEF1 expression is associated with papillary thyroid carcinoma. PMID: 28677753
    5. LEF1 was consistently expressed in the tubal-peritoneal junctions and all tubal intraepithelial lesions, independent of p53 status. PMID: 28664938
    6. these data suggest that LEF1 rather has tumor suppressive functions and attenuates aggressiveness in a subset of RMS. PMID: 27965462
    7. Positive LEF-1 favors a benign neoplasm. PMID: 28972308
    8. Results show that high enhancer-binding factor-1 (LEF1) expression is associated with poor survival for chronic lymphocytic leukemia (CLL) patients. PMID: 26950276
    9. Knockdown of GATA6 completely eliminated the effect of TCF1, while forced expression of GATA6 induced hESC differentiation PMID: 28943339
    10. Lef1 and Tcf1 showed oncogenic effect in colonic carcinogenesis. Cellular context of miRNAs might play important roles in carcinogenesis by altering the expression pattern of Lef/Tcfs members. PMID: 27433921
    11. Data indicate that microRNA miR-27a directly targets GSK-3beta and increases expression of beta-catenin and LEF1 in all-trans-retinoic acid (ATRA)-induced Hep2 cells. PMID: 28122350
    12. and CTU1/2, partner enzymes in U34 methoxycarbonylmethyl-2-thio tRNA modification, are up-regulated in human breast cancers and sustain metastasis. PMID: 27811057
    13. MicroRNA-557 might work as a tumor suppressor by negatively regulating the expression of lymphocyte enhancement factor 1 in lung cancer cells. PMID: 28639890
    14. EVI1 transcription is directly regulated by LEF1/beta-catenin complex in myeloid blast crisis of chronic myeloid leukemia. Loss of p53 function as a key regulator for beta-catenin-EVI1 in myeloid blast crisis of chronic myeloid leukemia. PMID: 27908728
    15. LEF1 is a sensitive and specific marker for chronic lymphocytic B-cell leukemia PMID: 28395058
    16. a frequency of 4-9% expression of LEF1 bymantle cell lymphoma PMID: 28038713
    17. The results suggest a role for beta-catenin/LEF1-mediated transcription in both malignant transformation and metastasis of prostate cancer PMID: 27067790
    18. The results describe a novel mutation in LEF1 causing dysregulation of Wnt/B catenin signaling in eyelid sebaceous cancinoma PMID: 25639559
    19. LEF-1 and MITF regulate tyrosinase gene transcription in vitro via binding to its promoter. PMID: 26580798
    20. Methylprednisolone can suppress Wnt signaling pathway by down-regulating LEF-1 protein expression in chronic lymphocyte leukemia cells. PMID: 26339357
    21. Overexpression of LEF1 is a favorable prognostic factor in childhood ALL. PMID: 25955539
    22. s found that the N-terminus of delta-catenin bound to the middle region of LEF-1 unlike beta-catenin. Overexpressed delta-catenin entered the nucleus and inhibited LEF-1-mediated transcriptional activity in Bosc23 and DLD-1 cell lines. PMID: 25808920
    23. high LEF1 expression and mutation are associated with high-risk leukemia and our results also revealed that LEF1 high expression and/or gain-of-function mutations are involved in leukemogenesis of ALL. PMID: 25942645
    24. Study found a negative correlation between miR-34a and LEF1 expression in prostate cancer cell lines and tumors and that miR-34a regulated epithelial-mesenchymal transition through direct binding to LEF1 mRNA 3' UTR region and silencing its translation. PMID: 25587085
    25. Our study suggests LEF1 expression in oral squamous cell carcinoma may play an important role in tumor progression and can be served as a predictor of poor prognosis for patients with oral squamous cell carcinoma. PMID: 24021930
    26. Elevated TCF-1 and LEF-1 expression is characteristic of malignant gliomas PMID: 24858819
    27. LEF-1 expression is associated with the presence of KRAS mutations and may have prognostic value as a trend of worse overall survival is seen in patients with LEF-1-positive colorectal carcinoma. PMID: 25394300
    28. These results further implicate the CTNNB1/LEF1 transcriptional complex in the development of solid-pseudopapillary neoplasms of the pancreas PMID: 24658583
    29. study identified TCF1 and LEF1 as Tax antagonistic factors in vivo, a fact which may critically influence the peripheral T-cell tropism of this virus PMID: 25646419
    30. miR26b may act as a potential therapeutic agent in reducing cancer cell proliferation through repressing LEF1 activation of c-Myc and cyclin D1 expression PMID: 24785257
    31. Tcf1 and Lef1 cooperate with Runx factors to achieve stable silencing of the Cd4 gene in CD8(+) T cells. PMID: 24847765
    32. LEF-1 expression in both basal cell adenomas and basal cell adenocarcinomas preferentially over other salivary gland tumors suggesting some utility as a diagnostic marker PMID: 25497834
    33. Proper sample processing ensured sufficient separation of positive LEF1 staining in T cells from negative staining in normal B and natural killer (NK) cells PMID: 25596247
    34. LEF1 expression is an independent prognostic factor in APL, and could be used in patients risk stratification. PMID: 24378360
    35. Selenite caused CYLD upregulation via LEF1 and cIAP downregulation, both of which contribute to the degradation of ubiquitin chains on RIP1 and subsequent caspase-8 activation and colorectal tumor cell apoptosis. PMID: 24577083
    36. Results indicate that lymphoid enhancer-binding factor 1 (LEF1) contributes to the pathophysiology of acute myeloid leukemia (AML) and could be a predictor of better treatment response. PMID: 23713453
    37. the expression of LEF1 is associated with the progression of human renal cell carcinoma and LEF1 maybe involved in the development of RCC PMID: 24897388
    38. By beta-catenin's association with LEF1 and BCL9-2/B9L. PMID: 24419084
    39. LEF1 protein was overexpressed in colon cancer tissues and knockdown of LEF1 expression inhibited colon cancer growth in vitro and in vivo. PMID: 24098538
    40. Studied the expression of TCF/LEF and SFRP family members (SFRP1 and SFRP3) to gain a better understanding of biological signaling pathways responsible for epidemiology and clinical parameters of clear cell RCC (cRCC). PMID: 23572277
    41. High LEF1 expression is associated with prostate cancer. PMID: 23913826
    42. DNA-dependent protein kinase catalytic subunit is a novel interaction partner of lymphocyte enhancer factor 1. PMID: 23325550
    43. This study supports a functionally important role for LEF1 and its target genes in Burkitt's lymphoma. PMID: 23375451
    44. this study demonstrated that miR-449a directly targets LEF-1, which in turn affects the expression of Sox 9, ultimately leading to the proper regulation of the differentiation and chondrogenesis PMID: 22769578
    45. Nuclear LEF1 correlate with cerebral metastasis of lung adenocarcinomas. PMID: 23224985
    46. LEF-1 might play an important role in colon carcinogenesis by acting as a regulator. PMID: 22639890
    47. Lef1 is overexpressed in human endometrial tumors, consistent with it playing a role in gland proliferation. PMID: 22792274
    48. An association between the maternal genotype and the occurrence of cleft lip or palate was observed at two polymorphic loci (rs10022956 and rs10025431), while a foetal-maternal effect modulating the risk of clefting was found at locus rs10025431. PMID: 21781440
    49. n-Butyl benzyl phthalate promotes breast cancer progression by inducing expression of lymphoid enhancer factor 1 PMID: 22905168
    50. interaction of HCLS1 with LEF-1 is essential for G-CSF-triggered myeloid differentiation PMID: 23001182

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TCF/LEF family
  • 组织特异性：
    Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several
  • 数据库链接：

    HGNC: 6551

    OMIM: 153245

    KEGG: hsa:51176

    STRING: 9606.ENSP00000265165

    UniGene: Hs.743478